Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system

Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system

An integrative vector system has been developed from the site-specific recombination elements of temperate phage 16-3. The system can be used for highly efficient stable introduction of genetic material into the chromosome of the symbiotic nitrogen-fixing organism, Rhizobium meliloti 41 ( Rm41) at t...

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Veröffentlicht in:Gene 1992-09, Vol.119 (1), p.9-15
Hauptverfasser: Hermesz, Edit, Olasz, Ferenc, Dorgai, László, Orosz, László
Format: Artikel
Sprache:eng
Schlagworte:
attachment sites
Bacteriophages
Chromosomes, Bacterial
Cloning, Molecular
Genetic Vectors
Genetics & Heredity
Int and Xis functions
Life Sciences & Biomedicine
molecular cloning
Plasmids
Recombinant DNA
Restriction Mapping
Rhizobium meliloti
Science & Technology
Sinorhizobium meliloti - genetics
site-specific recombination
temperate phage 16-3
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Beschreibung
Zusammenfassung:An integrative vector system has been developed from the site-specific recombination elements of temperate phage 16-3. The system can be used for highly efficient stable introduction of genetic material into the chromosome of the symbiotic nitrogen-fixing organism, Rhizobium meliloti 41 ( Rm41) at the attB site. Vectors carrying the phage-bome attachment site were constructed, and helper phages providing the site-specific recombination functions in trans were isolated. Other possible applications of the system are discussed.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(92)90061-S