State of the art of 2D DIGE
Difference gel electrophoresis enables the accurate quantification of changes in the proteome including combinations of PTMs and protein isoform expression. Here, we review recent advances in study design, image acquisition, and statistical analysis. We also compare DIGE to established and emerging...
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| Veröffentlicht in: | Proteomics. Clinical applications 2015-04, Vol.9 (3-4), p.277-288 |
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| container_title | Proteomics. Clinical applications |
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| creator | Arentz, Georgia Weiland, Florian Oehler, Martin K. Hoffmann, Peter |
| description | Difference gel electrophoresis enables the accurate quantification of changes in the proteome including combinations of PTMs and protein isoform expression. Here, we review recent advances in study design, image acquisition, and statistical analysis. We also compare DIGE to established and emerging mass spectrometric analysis technologies. Despite these recent advances in MS and the still unsolved limitations of 2DE to map hydrophobic, high molecular weight proteins with extreme pIs, DIGE remains the most comprehensive top‐down method to study changes in abundance of intact proteins. |
| doi_str_mv | 10.1002/prca.201400119 |
| format | Article |
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| ispartof | Proteomics. Clinical applications, 2015-04, Vol.9 (3-4), p.277-288 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 2DE DIGE Gel electrophoresis Mass Spectrometry Proteomics - methods Two-Dimensional Difference Gel Electrophoresis - methods |
| title | State of the art of 2D DIGE |
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