Development of microscopic time-domain dual lifetime referencing luminescence detection for pH monitoring in microfluidic free-flow isoelectric focusing

A lifetime‐based ratiometric microscale pH sensor system and a fluorescence microscopic setup was developed for the in‐line observation of pH in free‐flow isoelectric focusing based on the principle of time‐domain dual lifetime referencing (t‐DLR). The t‐DLR method has been developed for pH monitori...

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Veröffentlicht in:	Engineering in life sciences 2015-04, Vol.15 (3), p.276-285
Hauptverfasser:	Poehler, Elisabeth, Herzog, Christin, Suendermann, Madeleine, Pfeiffer, Simon A., Nagl, Stefan
Format:	Artikel
Sprache:	eng
Schlagworte:	Free-flow isoelectric focusing Luminescent pH sensor Microfluidic analytical systems Protein isoelectric point Time-domain dual lifetime referencing
Online-Zugang:	Volltext
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