Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model
The effects of estrogen deficiency on bone characteristics are site-dependent, with the most commonly studied sites being appendicular long bones (proximal femur and tibia) and axial bones (vertebra). The effect on the maxillary and mandibular bones is still inconsistent and requires further investi...
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| Veröffentlicht in: | Tissue engineering. Part C, Methods Methods, 2015-05, Vol.21 (5), p.458-466 |
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| creator | Du, Zhibin Steck, Roland Doan, Nghiem Woodruff, Maria Ann Ivanovski, Saso Xiao, Yin |
| description | The effects of estrogen deficiency on bone characteristics are site-dependent, with the most commonly studied sites being appendicular long bones (proximal femur and tibia) and axial bones (vertebra). The effect on the maxillary and mandibular bones is still inconsistent and requires further investigation. This study was designed to evaluate bone quality in the posterior maxilla of ovariectomized rats to validate this site as an appropriate model to study the effect of osteoporotic changes. Forty-eight 3-month-old female Sprague-Dawley rats were randomly divided into two groups: an ovariectomized (OVX) group (
n
=24) and Sham-operated (SHAM) group (
n
=24). Six rats were randomly sacrificed from both groups at time points 8, 12, 16, and 20 weeks. The samples from tibia and maxilla were collected for micro computed tomography (μCT) and histological analysis. For the maxilla, the volume of interest area focused on the furcation areas of the first and second molar. Trabecular bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.), and connectivity density (Conn.Dens) were analyzed after Micro CT scanning. At 8 weeks the indices BV/TV, Tb.Sp., Tb.N., and Conn.Dens showed significant differences (
p |
| doi_str_mv | 10.1089/ten.tec.2014.0268 |
| format | Article |
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n
=24) and Sham-operated (SHAM) group (
n
=24). Six rats were randomly sacrificed from both groups at time points 8, 12, 16, and 20 weeks. The samples from tibia and maxilla were collected for micro computed tomography (μCT) and histological analysis. For the maxilla, the volume of interest area focused on the furcation areas of the first and second molar. Trabecular bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.), and connectivity density (Conn.Dens) were analyzed after Micro CT scanning. At 8 weeks the indices BV/TV, Tb.Sp., Tb.N., and Conn.Dens showed significant differences (
p
<0.05) between the OVX and SHAM groups in the tibia. Compared with the tibia, the maxilla developed osteoporosis at a later stage, with significant changes in maxillary bone density only occurring after 12 weeks. Compared with the SHAM group, both the first and second molars of the OVX group showed significantly decreased BV/TV values from 12 weeks, and these changes were sustained through 16 and 20 weeks. For Tb.Sp., there were significant increases in bone values for the OVX group compared with the SHAM group at 12, 16, and 20 weeks. Histological changes were highly consistent with Micro CT results. This study established a method to quantify the changes of intra-radicular alveolar bone in the posterior maxilla in an accepted rat osteoporosis model. The degree of the osteoporotic changes to trabecular bone architecture is site-dependent and at least 3 months are required for the osteoporotic effects to be apparent in the posterior maxilla following rat OVX.</description><identifier>ISSN: 1937-3384</identifier><identifier>EISSN: 1937-3392</identifier><identifier>DOI: 10.1089/ten.tec.2014.0268</identifier><identifier>PMID: 25315176</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Body Weight ; Bone density ; Bone Density - drug effects ; Disease Models, Animal ; Estrogens ; Estrogens - deficiency ; Estrogens - physiology ; Female ; Maxilla - diagnostic imaging ; Maxilla - pathology ; Osteoporosis ; Osteoporosis - diagnostic imaging ; Osteoporosis - pathology ; Ovariectomy ; Rats ; Rats, Sprague-Dawley ; Rodents ; Tibia - diagnostic imaging ; Tibia - pathology ; Time Factors ; X-Ray Microtomography</subject><ispartof>Tissue engineering. Part C, Methods, 2015-05, Vol.21 (5), p.458-466</ispartof><rights>2015, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2015, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-f49063392fb3d6370b3ba2881e107fd2cf5a9ffa5eb3254ed7e7e899ef4939063</citedby><cites>FETCH-LOGICAL-c519t-f49063392fb3d6370b3ba2881e107fd2cf5a9ffa5eb3254ed7e7e899ef4939063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25315176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Zhibin</creatorcontrib><creatorcontrib>Steck, Roland</creatorcontrib><creatorcontrib>Doan, Nghiem</creatorcontrib><creatorcontrib>Woodruff, Maria Ann</creatorcontrib><creatorcontrib>Ivanovski, Saso</creatorcontrib><creatorcontrib>Xiao, Yin</creatorcontrib><title>Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model</title><title>Tissue engineering. Part C, Methods</title><addtitle>Tissue Eng Part C Methods</addtitle><description>The effects of estrogen deficiency on bone characteristics are site-dependent, with the most commonly studied sites being appendicular long bones (proximal femur and tibia) and axial bones (vertebra). The effect on the maxillary and mandibular bones is still inconsistent and requires further investigation. This study was designed to evaluate bone quality in the posterior maxilla of ovariectomized rats to validate this site as an appropriate model to study the effect of osteoporotic changes. Forty-eight 3-month-old female Sprague-Dawley rats were randomly divided into two groups: an ovariectomized (OVX) group (
n
=24) and Sham-operated (SHAM) group (
n
=24). Six rats were randomly sacrificed from both groups at time points 8, 12, 16, and 20 weeks. The samples from tibia and maxilla were collected for micro computed tomography (μCT) and histological analysis. For the maxilla, the volume of interest area focused on the furcation areas of the first and second molar. Trabecular bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.), and connectivity density (Conn.Dens) were analyzed after Micro CT scanning. At 8 weeks the indices BV/TV, Tb.Sp., Tb.N., and Conn.Dens showed significant differences (
p
<0.05) between the OVX and SHAM groups in the tibia. Compared with the tibia, the maxilla developed osteoporosis at a later stage, with significant changes in maxillary bone density only occurring after 12 weeks. Compared with the SHAM group, both the first and second molars of the OVX group showed significantly decreased BV/TV values from 12 weeks, and these changes were sustained through 16 and 20 weeks. For Tb.Sp., there were significant increases in bone values for the OVX group compared with the SHAM group at 12, 16, and 20 weeks. Histological changes were highly consistent with Micro CT results. This study established a method to quantify the changes of intra-radicular alveolar bone in the posterior maxilla in an accepted rat osteoporosis model. The degree of the osteoporotic changes to trabecular bone architecture is site-dependent and at least 3 months are required for the osteoporotic effects to be apparent in the posterior maxilla following rat OVX.</description><subject>Animals</subject><subject>Body Weight</subject><subject>Bone density</subject><subject>Bone Density - drug effects</subject><subject>Disease Models, Animal</subject><subject>Estrogens</subject><subject>Estrogens - deficiency</subject><subject>Estrogens - physiology</subject><subject>Female</subject><subject>Maxilla - diagnostic imaging</subject><subject>Maxilla - pathology</subject><subject>Osteoporosis</subject><subject>Osteoporosis - diagnostic imaging</subject><subject>Osteoporosis - pathology</subject><subject>Ovariectomy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Tibia - diagnostic imaging</subject><subject>Tibia - pathology</subject><subject>Time Factors</subject><subject>X-Ray Microtomography</subject><issn>1937-3384</issn><issn>1937-3392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks9uEzEQxlcIREvLA3BBlrhw2eA_u_aaWwiFVkoUFcF55fWOE1cbO9jeivBmvB3epu2hpx4sj0a_b8Yz_oriHcEzghv5KYGbJdAzikk1w5Q3L4pTIpkoGZP05WPcVCfFmxhvMOaYC_m6OKE1IzUR_LT4dxFT8Btw6CsYqy04fSjnMXptVYIeffEO0NLHiKxDaQtopf7YYVCf0RytIG197we_OaDk0fWoXLLmcIcttspt4KkqHNCVS0GVQfVWjzmB5sMt-Cm465Rx5dD6VgULOvmd_Zvf8EMltI4J_N4HH21EK9_DcF68MmqI8Pb-Pit-fbv4ubgsl-vvV4v5stQ1kak0lcR82ofpWM-ZwB3rFG0aAgQL01NtaiWNUTV0jNYV9AIENFJCFrJJelZ8PNbdB_97hJjanY0a8jQO_BhbwhtcVZhS-gxUcFZhUeGMfniC3vgxuDzIRNVUNDWXmSJHSufBYwDT7oPd5TW2BLeTBdpsgXx0O1mgnSyQNe_vK4_dDvpHxcOfZ0AcgSmtnBssdBDSM0r_B8lewto</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Du, Zhibin</creator><creator>Steck, Roland</creator><creator>Doan, Nghiem</creator><creator>Woodruff, Maria Ann</creator><creator>Ivanovski, Saso</creator><creator>Xiao, Yin</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20150501</creationdate><title>Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model</title><author>Du, Zhibin ; Steck, Roland ; Doan, Nghiem ; Woodruff, Maria Ann ; Ivanovski, Saso ; Xiao, Yin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-f49063392fb3d6370b3ba2881e107fd2cf5a9ffa5eb3254ed7e7e899ef4939063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Body Weight</topic><topic>Bone density</topic><topic>Bone Density - drug effects</topic><topic>Disease Models, Animal</topic><topic>Estrogens</topic><topic>Estrogens - deficiency</topic><topic>Estrogens - physiology</topic><topic>Female</topic><topic>Maxilla - diagnostic imaging</topic><topic>Maxilla - pathology</topic><topic>Osteoporosis</topic><topic>Osteoporosis - diagnostic imaging</topic><topic>Osteoporosis - pathology</topic><topic>Ovariectomy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Tibia - diagnostic imaging</topic><topic>Tibia - pathology</topic><topic>Time Factors</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Zhibin</creatorcontrib><creatorcontrib>Steck, Roland</creatorcontrib><creatorcontrib>Doan, Nghiem</creatorcontrib><creatorcontrib>Woodruff, Maria Ann</creatorcontrib><creatorcontrib>Ivanovski, Saso</creatorcontrib><creatorcontrib>Xiao, Yin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Tissue engineering. Part C, Methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Zhibin</au><au>Steck, Roland</au><au>Doan, Nghiem</au><au>Woodruff, Maria Ann</au><au>Ivanovski, Saso</au><au>Xiao, Yin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model</atitle><jtitle>Tissue engineering. Part C, Methods</jtitle><addtitle>Tissue Eng Part C Methods</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>21</volume><issue>5</issue><spage>458</spage><epage>466</epage><pages>458-466</pages><issn>1937-3384</issn><eissn>1937-3392</eissn><abstract>The effects of estrogen deficiency on bone characteristics are site-dependent, with the most commonly studied sites being appendicular long bones (proximal femur and tibia) and axial bones (vertebra). The effect on the maxillary and mandibular bones is still inconsistent and requires further investigation. This study was designed to evaluate bone quality in the posterior maxilla of ovariectomized rats to validate this site as an appropriate model to study the effect of osteoporotic changes. Forty-eight 3-month-old female Sprague-Dawley rats were randomly divided into two groups: an ovariectomized (OVX) group (
n
=24) and Sham-operated (SHAM) group (
n
=24). Six rats were randomly sacrificed from both groups at time points 8, 12, 16, and 20 weeks. The samples from tibia and maxilla were collected for micro computed tomography (μCT) and histological analysis. For the maxilla, the volume of interest area focused on the furcation areas of the first and second molar. Trabecular bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.), and connectivity density (Conn.Dens) were analyzed after Micro CT scanning. At 8 weeks the indices BV/TV, Tb.Sp., Tb.N., and Conn.Dens showed significant differences (
p
<0.05) between the OVX and SHAM groups in the tibia. Compared with the tibia, the maxilla developed osteoporosis at a later stage, with significant changes in maxillary bone density only occurring after 12 weeks. Compared with the SHAM group, both the first and second molars of the OVX group showed significantly decreased BV/TV values from 12 weeks, and these changes were sustained through 16 and 20 weeks. For Tb.Sp., there were significant increases in bone values for the OVX group compared with the SHAM group at 12, 16, and 20 weeks. Histological changes were highly consistent with Micro CT results. This study established a method to quantify the changes of intra-radicular alveolar bone in the posterior maxilla in an accepted rat osteoporosis model. The degree of the osteoporotic changes to trabecular bone architecture is site-dependent and at least 3 months are required for the osteoporotic effects to be apparent in the posterior maxilla following rat OVX.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>25315176</pmid><doi>10.1089/ten.tec.2014.0268</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3384 |
| ispartof | Tissue engineering. Part C, Methods, 2015-05, Vol.21 (5), p.458-466 |
| issn | 1937-3384 1937-3392 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1680440222 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Body Weight Bone density Bone Density - drug effects Disease Models, Animal Estrogens Estrogens - deficiency Estrogens - physiology Female Maxilla - diagnostic imaging Maxilla - pathology Osteoporosis Osteoporosis - diagnostic imaging Osteoporosis - pathology Ovariectomy Rats Rats, Sprague-Dawley Rodents Tibia - diagnostic imaging Tibia - pathology Time Factors X-Ray Microtomography |
| title | Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model |
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