Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing
KEY MESSAGE : Simultaneous gene silencing of both FAD2 genes in high linoleic acid flax leads to high level of oleic acid, which is stable across multiple generations. High oleic oil is one of the preferred traits in oil crop engineering due to its stability and multiple applications as an industria...
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| Veröffentlicht in: | Plant cell reports 2015-04, Vol.34 (4), p.643-653 |
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| creator | Chen, Yurong Zhou, Xue-Rong Zhang, Zhi-Jun Dribnenki, Paul Singh, Surinder Green, Allan |
| description | KEY MESSAGE : Simultaneous gene silencing of both FAD2 genes in high linoleic acid flax leads to high level of oleic acid, which is stable across multiple generations. High oleic oil is one of the preferred traits in oil crop engineering due to its stability and multiple applications as an industrial feedstock. Flax possesses two isoforms of FAD2 enzymes that desaturate monounsaturated oleic acid to polyunsaturated linoleic acid. These two enzymes are encoded by two FAD2 genes. By simultaneous gene silencing both FAD2 genes in high linoleic acid flax, Linola, high level of oleic acid up to 80 % was achieved in 69 silencing lines. The high oleic trait was stable across multiple generations with oleic acid reaching up to 77 % in homozygote T3 progeny. The RNAi-mediated gene-silencing approach generated high oleic linseed oil, as well as a high oleic platform that can be exploited for further fatty acid engineering. |
| doi_str_mv | 10.1007/s00299-015-1737-5 |
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High oleic oil is one of the preferred traits in oil crop engineering due to its stability and multiple applications as an industrial feedstock. Flax possesses two isoforms of FAD2 enzymes that desaturate monounsaturated oleic acid to polyunsaturated linoleic acid. These two enzymes are encoded by two FAD2 genes. By simultaneous gene silencing both FAD2 genes in high linoleic acid flax, Linola, high level of oleic acid up to 80 % was achieved in 69 silencing lines. The high oleic trait was stable across multiple generations with oleic acid reaching up to 77 % in homozygote T3 progeny. The RNAi-mediated gene-silencing approach generated high oleic linseed oil, as well as a high oleic platform that can be exploited for further fatty acid engineering.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-015-1737-5</identifier><identifier>PMID: 25604988</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Base Sequence ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Chromosome Segregation ; Crops, Agricultural - genetics ; enzymes ; Fatty Acid Desaturases - genetics ; feedstocks ; flax ; Flax - enzymology ; Flax - genetics ; Gene Expression Regulation, Plant ; gene silencing ; genes ; Genes, Plant ; Inheritance Patterns - genetics ; Life Sciences ; linoleic acid ; linseed oil ; Molecular Sequence Data ; oleic acid ; Oleic Acid - metabolism ; Original Paper ; Plant Biochemistry ; Plant Sciences ; Plants, Genetically Modified ; progeny ; Quantitative Trait, Heritable ; RNA Interference ; Seeds - genetics ; Sequence Homology, Nucleic Acid ; Transformation, Genetic</subject><ispartof>Plant cell reports, 2015-04, Vol.34 (4), p.643-653</ispartof><rights>Crown Copyright 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-16820c6276ee2464d65f1c6a3075f04208d80e32cd9e6cdafb674bd7be0613183</citedby><cites>FETCH-LOGICAL-c565t-16820c6276ee2464d65f1c6a3075f04208d80e32cd9e6cdafb674bd7be0613183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-015-1737-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-015-1737-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25604988$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yurong</creatorcontrib><creatorcontrib>Zhou, Xue-Rong</creatorcontrib><creatorcontrib>Zhang, Zhi-Jun</creatorcontrib><creatorcontrib>Dribnenki, Paul</creatorcontrib><creatorcontrib>Singh, Surinder</creatorcontrib><creatorcontrib>Green, Allan</creatorcontrib><title>Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>KEY MESSAGE : Simultaneous gene silencing of both FAD2 genes in high linoleic acid flax leads to high level of oleic acid, which is stable across multiple generations. High oleic oil is one of the preferred traits in oil crop engineering due to its stability and multiple applications as an industrial feedstock. Flax possesses two isoforms of FAD2 enzymes that desaturate monounsaturated oleic acid to polyunsaturated linoleic acid. These two enzymes are encoded by two FAD2 genes. By simultaneous gene silencing both FAD2 genes in high linoleic acid flax, Linola, high level of oleic acid up to 80 % was achieved in 69 silencing lines. The high oleic trait was stable across multiple generations with oleic acid reaching up to 77 % in homozygote T3 progeny. The RNAi-mediated gene-silencing approach generated high oleic linseed oil, as well as a high oleic platform that can be exploited for further fatty acid engineering.</description><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Chromosome Segregation</subject><subject>Crops, Agricultural - genetics</subject><subject>enzymes</subject><subject>Fatty Acid Desaturases - genetics</subject><subject>feedstocks</subject><subject>flax</subject><subject>Flax - enzymology</subject><subject>Flax - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene silencing</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Inheritance Patterns - genetics</subject><subject>Life Sciences</subject><subject>linoleic acid</subject><subject>linseed oil</subject><subject>Molecular Sequence Data</subject><subject>oleic acid</subject><subject>Oleic Acid - metabolism</subject><subject>Original Paper</subject><subject>Plant Biochemistry</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified</subject><subject>progeny</subject><subject>Quantitative Trait, Heritable</subject><subject>RNA Interference</subject><subject>Seeds - 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genetics</topic><topic>enzymes</topic><topic>Fatty Acid Desaturases - genetics</topic><topic>feedstocks</topic><topic>flax</topic><topic>Flax - enzymology</topic><topic>Flax - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene silencing</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Inheritance Patterns - genetics</topic><topic>Life Sciences</topic><topic>linoleic acid</topic><topic>linseed oil</topic><topic>Molecular Sequence Data</topic><topic>oleic acid</topic><topic>Oleic Acid - metabolism</topic><topic>Original Paper</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified</topic><topic>progeny</topic><topic>Quantitative Trait, Heritable</topic><topic>RNA Interference</topic><topic>Seeds - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yurong</creatorcontrib><creatorcontrib>Zhou, Xue-Rong</creatorcontrib><creatorcontrib>Zhang, Zhi-Jun</creatorcontrib><creatorcontrib>Dribnenki, Paul</creatorcontrib><creatorcontrib>Singh, Surinder</creatorcontrib><creatorcontrib>Green, Allan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yurong</au><au>Zhou, Xue-Rong</au><au>Zhang, Zhi-Jun</au><au>Dribnenki, Paul</au><au>Singh, Surinder</au><au>Green, Allan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>34</volume><issue>4</issue><spage>643</spage><epage>653</epage><pages>643-653</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>KEY MESSAGE : Simultaneous gene silencing of both FAD2 genes in high linoleic acid flax leads to high level of oleic acid, which is stable across multiple generations. High oleic oil is one of the preferred traits in oil crop engineering due to its stability and multiple applications as an industrial feedstock. Flax possesses two isoforms of FAD2 enzymes that desaturate monounsaturated oleic acid to polyunsaturated linoleic acid. These two enzymes are encoded by two FAD2 genes. By simultaneous gene silencing both FAD2 genes in high linoleic acid flax, Linola, high level of oleic acid up to 80 % was achieved in 69 silencing lines. The high oleic trait was stable across multiple generations with oleic acid reaching up to 77 % in homozygote T3 progeny. The RNAi-mediated gene-silencing approach generated high oleic linseed oil, as well as a high oleic platform that can be exploited for further fatty acid engineering.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>25604988</pmid><doi>10.1007/s00299-015-1737-5</doi><tpages>11</tpages></addata></record> |
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| subjects | Base Sequence Biomedical and Life Sciences Biotechnology Cell Biology Chromosome Segregation Crops, Agricultural - genetics enzymes Fatty Acid Desaturases - genetics feedstocks flax Flax - enzymology Flax - genetics Gene Expression Regulation, Plant gene silencing genes Genes, Plant Inheritance Patterns - genetics Life Sciences linoleic acid linseed oil Molecular Sequence Data oleic acid Oleic Acid - metabolism Original Paper Plant Biochemistry Plant Sciences Plants, Genetically Modified progeny Quantitative Trait, Heritable RNA Interference Seeds - genetics Sequence Homology, Nucleic Acid Transformation, Genetic |
| title | Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing |
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