Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections
The objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. To achieve this objective, a joint experimental strategy was established among the different involved partner collections of...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2015-04, Vol.99 (8), p.3559-3571 |
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| creator | Peiren, Jindrich Buyse, Joke De Vos, Paul Lang, Elke Clermont, Dominique Hamon, Sylviane Bégaud, Evelyne Bizet, Chantal Pascual, Javier Ruvira, María A Macián, M. Carmen Arahal, David R |
| description | The objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. To achieve this objective, a joint experimental strategy was established among the different involved partner collections of the EMbaRC project (www.embarc.eu). Five bacterial strains considered as recalcitrant to freeze-drying were subjected to a standardized freeze-drying protocol and to seven agreed protocol variants. Viability of these strains was determined before and after freeze-drying (within 1 week, after 6 and 12 months, and after accelerated storage) for each of the protocols. Furthermore, strains were exchanged between partners to perform experiments with different freeze-dryer-dependent parameters. Of all tested variables, choice of the lyoprotectant had the biggest impact on viability after freeze-drying and during storage. For nearly all tested strains, skim milk as lyoprotectant resulted in lowest viability after freeze-drying and storage. On the other hand, best freeze-drying and storage conditions were strain and device dependent. For Aeromonas salmonicida CECT 894ᵀ, best survival was obtained when horse serum supplemented with trehalose was used as lyoprotectant, while Aliivibrio fischeri LMG 4414ᵀshould be freeze-dried in skim milk supplemented with marine broth in a 1:1 ratio. Freeze-drying Campylobacter fetus CIP 53.96ᵀusing skim milk supplemented with trehalose as lyoprotectant resulted in best recovery. Xanthomonas fragariae DSM 3587ᵀexpressed high viability after freeze-drying and storage for all tested lyoprotectants and could not be considered as recalcitrant. In contrary, Flavobacterium columnare LMG 10406ᵀdid not survive the freeze-drying process under all tested conditions. |
| doi_str_mv | 10.1007/s00253-015-6476-6 |
| format | Article |
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Carmen ; Arahal, David R</creator><creatorcontrib>Peiren, Jindrich ; Buyse, Joke ; De Vos, Paul ; Lang, Elke ; Clermont, Dominique ; Hamon, Sylviane ; Bégaud, Evelyne ; Bizet, Chantal ; Pascual, Javier ; Ruvira, María A ; Macián, M. Carmen ; Arahal, David R</creatorcontrib><description>The objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. To achieve this objective, a joint experimental strategy was established among the different involved partner collections of the EMbaRC project (www.embarc.eu). Five bacterial strains considered as recalcitrant to freeze-drying were subjected to a standardized freeze-drying protocol and to seven agreed protocol variants. Viability of these strains was determined before and after freeze-drying (within 1 week, after 6 and 12 months, and after accelerated storage) for each of the protocols. Furthermore, strains were exchanged between partners to perform experiments with different freeze-dryer-dependent parameters. Of all tested variables, choice of the lyoprotectant had the biggest impact on viability after freeze-drying and during storage. For nearly all tested strains, skim milk as lyoprotectant resulted in lowest viability after freeze-drying and storage. On the other hand, best freeze-drying and storage conditions were strain and device dependent. For Aeromonas salmonicida CECT 894ᵀ, best survival was obtained when horse serum supplemented with trehalose was used as lyoprotectant, while Aliivibrio fischeri LMG 4414ᵀshould be freeze-dried in skim milk supplemented with marine broth in a 1:1 ratio. Freeze-drying Campylobacter fetus CIP 53.96ᵀusing skim milk supplemented with trehalose as lyoprotectant resulted in best recovery. Xanthomonas fragariae DSM 3587ᵀexpressed high viability after freeze-drying and storage for all tested lyoprotectants and could not be considered as recalcitrant. In contrary, Flavobacterium columnare LMG 10406ᵀdid not survive the freeze-drying process under all tested conditions.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-015-6476-6</identifier><identifier>PMID: 25773973</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Aeromonas salmonicida ; Analysis ; Bacteria ; Bacterial Physiological Phenomena - radiation effects ; Biological Specimen Banks ; Biomedical and Life Sciences ; Biotechnology ; blood serum ; Campylobacter ; Campylobacter fetus ; Collections ; Cryopreservation ; Cryopreservation of organs, tissues, etc ; European culture ; Flavobacterium columnare ; Freeze drying ; Freeze Drying - methods ; Freezing ; horses ; Influence ; Life Sciences ; Methods and Protocols ; Microbial Genetics and Genomics ; Microbial Viability - radiation effects ; Microbiology ; Milk ; Moisture content ; Physiological aspects ; Physiology ; Preservation, Biological - methods ; skim milk ; storage conditions ; storage quality ; Studies ; Temperature ; trehalose ; Variables ; viability ; Vibrio fischeri ; Xanthomonas fragariae</subject><ispartof>Applied microbiology and biotechnology, 2015-04, Vol.99 (8), p.3559-3571</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>COPYRIGHT 2015 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c637t-cc48f3b9a3e2adf299705d21d4f1875c9f86322c07c8dd14a389a0d193ddea7c3</citedby><cites>FETCH-LOGICAL-c637t-cc48f3b9a3e2adf299705d21d4f1875c9f86322c07c8dd14a389a0d193ddea7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-015-6476-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-015-6476-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25773973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peiren, Jindrich</creatorcontrib><creatorcontrib>Buyse, Joke</creatorcontrib><creatorcontrib>De Vos, Paul</creatorcontrib><creatorcontrib>Lang, Elke</creatorcontrib><creatorcontrib>Clermont, Dominique</creatorcontrib><creatorcontrib>Hamon, Sylviane</creatorcontrib><creatorcontrib>Bégaud, Evelyne</creatorcontrib><creatorcontrib>Bizet, Chantal</creatorcontrib><creatorcontrib>Pascual, Javier</creatorcontrib><creatorcontrib>Ruvira, María A</creatorcontrib><creatorcontrib>Macián, M. Carmen</creatorcontrib><creatorcontrib>Arahal, David R</creatorcontrib><title>Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. To achieve this objective, a joint experimental strategy was established among the different involved partner collections of the EMbaRC project (www.embarc.eu). Five bacterial strains considered as recalcitrant to freeze-drying were subjected to a standardized freeze-drying protocol and to seven agreed protocol variants. Viability of these strains was determined before and after freeze-drying (within 1 week, after 6 and 12 months, and after accelerated storage) for each of the protocols. Furthermore, strains were exchanged between partners to perform experiments with different freeze-dryer-dependent parameters. Of all tested variables, choice of the lyoprotectant had the biggest impact on viability after freeze-drying and during storage. For nearly all tested strains, skim milk as lyoprotectant resulted in lowest viability after freeze-drying and storage. On the other hand, best freeze-drying and storage conditions were strain and device dependent. For Aeromonas salmonicida CECT 894ᵀ, best survival was obtained when horse serum supplemented with trehalose was used as lyoprotectant, while Aliivibrio fischeri LMG 4414ᵀshould be freeze-dried in skim milk supplemented with marine broth in a 1:1 ratio. Freeze-drying Campylobacter fetus CIP 53.96ᵀusing skim milk supplemented with trehalose as lyoprotectant resulted in best recovery. Xanthomonas fragariae DSM 3587ᵀexpressed high viability after freeze-drying and storage for all tested lyoprotectants and could not be considered as recalcitrant. In contrary, Flavobacterium columnare LMG 10406ᵀdid not survive the freeze-drying process under all tested conditions.</description><subject>Aeromonas salmonicida</subject><subject>Analysis</subject><subject>Bacteria</subject><subject>Bacterial Physiological Phenomena - radiation effects</subject><subject>Biological Specimen Banks</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>blood serum</subject><subject>Campylobacter</subject><subject>Campylobacter fetus</subject><subject>Collections</subject><subject>Cryopreservation</subject><subject>Cryopreservation of organs, tissues, etc</subject><subject>European culture</subject><subject>Flavobacterium columnare</subject><subject>Freeze drying</subject><subject>Freeze Drying - methods</subject><subject>Freezing</subject><subject>horses</subject><subject>Influence</subject><subject>Life Sciences</subject><subject>Methods and Protocols</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbial Viability - radiation effects</subject><subject>Microbiology</subject><subject>Milk</subject><subject>Moisture content</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Preservation, Biological - methods</subject><subject>skim milk</subject><subject>storage conditions</subject><subject>storage quality</subject><subject>Studies</subject><subject>Temperature</subject><subject>trehalose</subject><subject>Variables</subject><subject>viability</subject><subject>Vibrio fischeri</subject><subject>Xanthomonas fragariae</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl9rFDEUxQdR7Fr9AL5owBd9mJo_M8mMb6VUXSgI1j6Hu8nNkDI7WZPM4voF_Npm2SpdEZE8XEh-58C5OVX1nNEzRql6myjlragpa2vZKFnLB9WCNYLXVLLmYbWgTLW1avvupHqS0i2ljHdSPq5OeKuU6JVYVD-W600MWz8NJM1x67cwEpgsSTlEGLBMWPnR5x0JjqzAZIweSEQDo_E5wpRJDsRFxO9Y27grRu8IEBNCtH6CjHur2e7Iakcu5xg2CBMx85jniIUaRzTZhyk9rR45GBM-u5un1c37yy8XH-urTx-WF-dXtZFC5dqYpnNi1YNADtbxvle0tZzZxrFOtaZ3nRScG6pMZy1rQHQ9UMt6YS2CMuK0en3wLam_zpiyXvtkcBxhwjAnzWRHG9Hyhv8HKpVoes5pQV_9gd6GOU4lyJ7qWHFV96gBRtR-cqEs0OxN9XnDe0k5E22hzv5ClWNx7U2Y0PlyfyR4cyQoTMZveYA5Jb28_nzMsgNrYkgpotOb6NcQd5pRve-UPnRKl07pfae0LJoXd-Hm1Rrtb8WvEhWAH4BUnqYB4730_3B9eRA5CBqG6JO-ueYFKC0tX9314ie5kN90</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Peiren, Jindrich</creator><creator>Buyse, Joke</creator><creator>De Vos, Paul</creator><creator>Lang, Elke</creator><creator>Clermont, Dominique</creator><creator>Hamon, Sylviane</creator><creator>Bégaud, Evelyne</creator><creator>Bizet, Chantal</creator><creator>Pascual, Javier</creator><creator>Ruvira, María A</creator><creator>Macián, M. 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Carmen</au><au>Arahal, David R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>99</volume><issue>8</issue><spage>3559</spage><epage>3571</epage><pages>3559-3571</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>The objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. To achieve this objective, a joint experimental strategy was established among the different involved partner collections of the EMbaRC project (www.embarc.eu). Five bacterial strains considered as recalcitrant to freeze-drying were subjected to a standardized freeze-drying protocol and to seven agreed protocol variants. Viability of these strains was determined before and after freeze-drying (within 1 week, after 6 and 12 months, and after accelerated storage) for each of the protocols. Furthermore, strains were exchanged between partners to perform experiments with different freeze-dryer-dependent parameters. Of all tested variables, choice of the lyoprotectant had the biggest impact on viability after freeze-drying and during storage. For nearly all tested strains, skim milk as lyoprotectant resulted in lowest viability after freeze-drying and storage. On the other hand, best freeze-drying and storage conditions were strain and device dependent. For Aeromonas salmonicida CECT 894ᵀ, best survival was obtained when horse serum supplemented with trehalose was used as lyoprotectant, while Aliivibrio fischeri LMG 4414ᵀshould be freeze-dried in skim milk supplemented with marine broth in a 1:1 ratio. Freeze-drying Campylobacter fetus CIP 53.96ᵀusing skim milk supplemented with trehalose as lyoprotectant resulted in best recovery. Xanthomonas fragariae DSM 3587ᵀexpressed high viability after freeze-drying and storage for all tested lyoprotectants and could not be considered as recalcitrant. In contrary, Flavobacterium columnare LMG 10406ᵀdid not survive the freeze-drying process under all tested conditions.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>25773973</pmid><doi>10.1007/s00253-015-6476-6</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2015-04, Vol.99 (8), p.3559-3571 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1680435242 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Aeromonas salmonicida Analysis Bacteria Bacterial Physiological Phenomena - radiation effects Biological Specimen Banks Biomedical and Life Sciences Biotechnology blood serum Campylobacter Campylobacter fetus Collections Cryopreservation Cryopreservation of organs, tissues, etc European culture Flavobacterium columnare Freeze drying Freeze Drying - methods Freezing horses Influence Life Sciences Methods and Protocols Microbial Genetics and Genomics Microbial Viability - radiation effects Microbiology Milk Moisture content Physiological aspects Physiology Preservation, Biological - methods skim milk storage conditions storage quality Studies Temperature trehalose Variables viability Vibrio fischeri Xanthomonas fragariae |
| title | Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T17%3A04%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improving%20survival%20and%20storage%20stability%20of%20bacteria%20recalcitrant%20to%20freeze-drying:%20a%20coordinated%20study%20by%20European%20culture%20collections&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Peiren,%20Jindrich&rft.date=2015-04-01&rft.volume=99&rft.issue=8&rft.spage=3559&rft.epage=3571&rft.pages=3559-3571&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-015-6476-6&rft_dat=%3Cgale_proqu%3EA429602135%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1668116870&rft_id=info:pmid/25773973&rft_galeid=A429602135&rfr_iscdi=true |