Regulation of interleukin-8 expression in porcine alveolar macrophages by bacterial lipopolysaccharide
Interleukin (IL)-8 is a macrophage-derived neutrophil chemotactic factor that plays an important role in the recruitment of neutrophils to inflammatory loci. Hence, expression of IL-8 by alveolar macrophages may be a significant factor in host defense in the lung and in the pathogenesis of pneumonia...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-01, Vol.269 (1), p.77-85 |
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| creator | Lin, G Pearson, A E Scamurra, R W Zhou, Y Baarsch, M J Weiss, D J Murtaugh, M P |
| description | Interleukin (IL)-8 is a macrophage-derived neutrophil chemotactic factor that plays an important role in the recruitment of
neutrophils to inflammatory loci. Hence, expression of IL-8 by alveolar macrophages may be a significant factor in host defense
in the lung and in the pathogenesis of pneumonia in swine. To initiate molecular studies of IL-8 regulation in pigs, we cloned
IL-8 cDNA and examined the regulation of its mRNA in alveolar macrophages. The porcine IL-8 cDNA consists of 1491 base pairs
including a coding region of 309 base pairs. The deduced amino acid sequence was 75 and 81% similar to human and rabbit IL-8,
respectively. Resting macrophages contained low levels of IL-8 mRNA, which increased markedly after exposure to bacterial
lipopolysaccharide (LPS). LPS induction of IL-8 was direct, not mediated through elevation of tumor necrosis factor or interleukin-1.
The effect of LPS on IL-8 expression was dose dependent, and induction was observed at a concentration of 10 pg/ml. IL-8 mRNA
expression was detectable within 0.5 h after stimulation with LPS, peaked at 3-6 h at about 30-fold higher levels than in
resting cells, and was maintained for 24 h. Secreted IL-8, measured by neutrophil chemotaxis, was induced within 4 h by LPS,
and accumulated in the media throughout the 24-h period. The mechanism of induction of IL-8 mRNA appeared to involve transcription
and RNA processing. Nuclear run-on analysis showed that the IL-8 gene was actively transcribed in noninduced cells; upon stimulation
with LPS, the rate of IL-8 transcription was increased about 4-fold. A single mature mRNA species was detected by primer extension
analysis. The half-life of IL-8 mRNA transcripts in aveolar macrophages was approximately 2 h and did not change after LPS
stimulation. The ability of LPS to induce IL-8 expression was suppressed by recombinant human IL-4 and dexamethasone in a
concentration-dependent manner. These observations indicate that the expression of IL-8 is an early event in the sequelae
to bacterial infection in the lung. |
| doi_str_mv | 10.1016/s0021-9258(17)42316-7 |
| format | Article |
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neutrophils to inflammatory loci. Hence, expression of IL-8 by alveolar macrophages may be a significant factor in host defense
in the lung and in the pathogenesis of pneumonia in swine. To initiate molecular studies of IL-8 regulation in pigs, we cloned
IL-8 cDNA and examined the regulation of its mRNA in alveolar macrophages. The porcine IL-8 cDNA consists of 1491 base pairs
including a coding region of 309 base pairs. The deduced amino acid sequence was 75 and 81% similar to human and rabbit IL-8,
respectively. Resting macrophages contained low levels of IL-8 mRNA, which increased markedly after exposure to bacterial
lipopolysaccharide (LPS). LPS induction of IL-8 was direct, not mediated through elevation of tumor necrosis factor or interleukin-1.
The effect of LPS on IL-8 expression was dose dependent, and induction was observed at a concentration of 10 pg/ml. IL-8 mRNA
expression was detectable within 0.5 h after stimulation with LPS, peaked at 3-6 h at about 30-fold higher levels than in
resting cells, and was maintained for 24 h. Secreted IL-8, measured by neutrophil chemotaxis, was induced within 4 h by LPS,
and accumulated in the media throughout the 24-h period. The mechanism of induction of IL-8 mRNA appeared to involve transcription
and RNA processing. Nuclear run-on analysis showed that the IL-8 gene was actively transcribed in noninduced cells; upon stimulation
with LPS, the rate of IL-8 transcription was increased about 4-fold. A single mature mRNA species was detected by primer extension
analysis. The half-life of IL-8 mRNA transcripts in aveolar macrophages was approximately 2 h and did not change after LPS
stimulation. The ability of LPS to induce IL-8 expression was suppressed by recombinant human IL-4 and dexamethasone in a
concentration-dependent manner. These observations indicate that the expression of IL-8 is an early event in the sequelae
to bacterial infection in the lung.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(17)42316-7</identifier><identifier>PMID: 8276881</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Analysis of the immune response. Humoral and cellular immunity ; Animals ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Dexamethasone - pharmacology ; DNA, Complementary ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Expression Regulation - drug effects ; Humans ; Immunobiology ; Interleukin-4 - pharmacology ; Interleukin-8 - biosynthesis ; Interleukin-8 - genetics ; Lipopolysaccharides - pharmacology ; Lymphokines, interleukins ( function, expression) ; Macrophages, Alveolar - metabolism ; Molecular Sequence Data ; Regulatory factors and their cellular receptors ; Sequence Homology, Amino Acid ; Swine ; Transcription, Genetic</subject><ispartof>The Journal of biological chemistry, 1994-01, Vol.269 (1), p.77-85</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-45905fd239af11e698dcbb98d1fe0d287c13c2f162304812bcd6890512548ba13</citedby><cites>FETCH-LOGICAL-c502t-45905fd239af11e698dcbb98d1fe0d287c13c2f162304812bcd6890512548ba13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3962860$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8276881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, G</creatorcontrib><creatorcontrib>Pearson, A E</creatorcontrib><creatorcontrib>Scamurra, R W</creatorcontrib><creatorcontrib>Zhou, Y</creatorcontrib><creatorcontrib>Baarsch, M J</creatorcontrib><creatorcontrib>Weiss, D J</creatorcontrib><creatorcontrib>Murtaugh, M P</creatorcontrib><title>Regulation of interleukin-8 expression in porcine alveolar macrophages by bacterial lipopolysaccharide</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Interleukin (IL)-8 is a macrophage-derived neutrophil chemotactic factor that plays an important role in the recruitment of
neutrophils to inflammatory loci. Hence, expression of IL-8 by alveolar macrophages may be a significant factor in host defense
in the lung and in the pathogenesis of pneumonia in swine. To initiate molecular studies of IL-8 regulation in pigs, we cloned
IL-8 cDNA and examined the regulation of its mRNA in alveolar macrophages. The porcine IL-8 cDNA consists of 1491 base pairs
including a coding region of 309 base pairs. The deduced amino acid sequence was 75 and 81% similar to human and rabbit IL-8,
respectively. Resting macrophages contained low levels of IL-8 mRNA, which increased markedly after exposure to bacterial
lipopolysaccharide (LPS). LPS induction of IL-8 was direct, not mediated through elevation of tumor necrosis factor or interleukin-1.
The effect of LPS on IL-8 expression was dose dependent, and induction was observed at a concentration of 10 pg/ml. IL-8 mRNA
expression was detectable within 0.5 h after stimulation with LPS, peaked at 3-6 h at about 30-fold higher levels than in
resting cells, and was maintained for 24 h. Secreted IL-8, measured by neutrophil chemotaxis, was induced within 4 h by LPS,
and accumulated in the media throughout the 24-h period. The mechanism of induction of IL-8 mRNA appeared to involve transcription
and RNA processing. Nuclear run-on analysis showed that the IL-8 gene was actively transcribed in noninduced cells; upon stimulation
with LPS, the rate of IL-8 transcription was increased about 4-fold. A single mature mRNA species was detected by primer extension
analysis. The half-life of IL-8 mRNA transcripts in aveolar macrophages was approximately 2 h and did not change after LPS
stimulation. The ability of LPS to induce IL-8 expression was suppressed by recombinant human IL-4 and dexamethasone in a
concentration-dependent manner. These observations indicate that the expression of IL-8 is an early event in the sequelae
to bacterial infection in the lung.</description><subject>Amino Acid Sequence</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Dexamethasone - pharmacology</subject><subject>DNA, Complementary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Interleukin-4 - pharmacology</subject><subject>Interleukin-8 - biosynthesis</subject><subject>Interleukin-8 - genetics</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Macrophages, Alveolar - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Sequence Homology, Amino Acid</subject><subject>Swine</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1u1DAURi1UVKaFR6jkBaroIuBrJ46zrKrSIlVC4kdiZznO9cTgxKk9AebtSZjReHG9-M53LR9CroC9BwbyQ2aMQ9HwSr2D-qbkAmRRvyAbYEoUooIfZ2RzQl6Ri5x_suWUDZyTc8VrqRRsiPuC2zmYnY8jjY76cYcp4PzLj4Wi-HdKmPOa-ZFOMVk_IjXhN8ZgEh2MTXHqzRYzbfe0NXYpexNo8FOcYthnY21vku_wNXnpTMj45nhfku8f77_dPRZPnx8-3d0-FbZifFeUVcMq13HRGAeAslGdbdtlgkPWcVVbEJY7kFywUgFvbSfVUgFelao1IC7J9WHvlOLzjHmnB58thmBGjHPWIBXjFdQLWB3A5Qs5J3R6Sn4waa-B6dWv_rrK06s8DbX-71evvavjA3M7YHdqHYUu-dtjbrI1wSUzWp9PmGgkV5ItGD1gvd_2f3xC3fpoexw0l40GXdfiHzz7j0Y</recordid><startdate>19940107</startdate><enddate>19940107</enddate><creator>Lin, G</creator><creator>Pearson, A E</creator><creator>Scamurra, R W</creator><creator>Zhou, Y</creator><creator>Baarsch, M J</creator><creator>Weiss, D J</creator><creator>Murtaugh, M P</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19940107</creationdate><title>Regulation of interleukin-8 expression in porcine alveolar macrophages by bacterial lipopolysaccharide</title><author>Lin, G ; Pearson, A E ; Scamurra, R W ; Zhou, Y ; Baarsch, M J ; Weiss, D J ; Murtaugh, M P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-45905fd239af11e698dcbb98d1fe0d287c13c2f162304812bcd6890512548ba13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Dexamethasone - pharmacology</topic><topic>DNA, Complementary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Interleukin-4 - pharmacology</topic><topic>Interleukin-8 - biosynthesis</topic><topic>Interleukin-8 - genetics</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Macrophages, Alveolar - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Sequence Homology, Amino Acid</topic><topic>Swine</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, G</creatorcontrib><creatorcontrib>Pearson, A E</creatorcontrib><creatorcontrib>Scamurra, R W</creatorcontrib><creatorcontrib>Zhou, Y</creatorcontrib><creatorcontrib>Baarsch, M J</creatorcontrib><creatorcontrib>Weiss, D J</creatorcontrib><creatorcontrib>Murtaugh, M P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, G</au><au>Pearson, A E</au><au>Scamurra, R W</au><au>Zhou, Y</au><au>Baarsch, M J</au><au>Weiss, D J</au><au>Murtaugh, M P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of interleukin-8 expression in porcine alveolar macrophages by bacterial lipopolysaccharide</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-01-07</date><risdate>1994</risdate><volume>269</volume><issue>1</issue><spage>77</spage><epage>85</epage><pages>77-85</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Interleukin (IL)-8 is a macrophage-derived neutrophil chemotactic factor that plays an important role in the recruitment of
neutrophils to inflammatory loci. Hence, expression of IL-8 by alveolar macrophages may be a significant factor in host defense
in the lung and in the pathogenesis of pneumonia in swine. To initiate molecular studies of IL-8 regulation in pigs, we cloned
IL-8 cDNA and examined the regulation of its mRNA in alveolar macrophages. The porcine IL-8 cDNA consists of 1491 base pairs
including a coding region of 309 base pairs. The deduced amino acid sequence was 75 and 81% similar to human and rabbit IL-8,
respectively. Resting macrophages contained low levels of IL-8 mRNA, which increased markedly after exposure to bacterial
lipopolysaccharide (LPS). LPS induction of IL-8 was direct, not mediated through elevation of tumor necrosis factor or interleukin-1.
The effect of LPS on IL-8 expression was dose dependent, and induction was observed at a concentration of 10 pg/ml. IL-8 mRNA
expression was detectable within 0.5 h after stimulation with LPS, peaked at 3-6 h at about 30-fold higher levels than in
resting cells, and was maintained for 24 h. Secreted IL-8, measured by neutrophil chemotaxis, was induced within 4 h by LPS,
and accumulated in the media throughout the 24-h period. The mechanism of induction of IL-8 mRNA appeared to involve transcription
and RNA processing. Nuclear run-on analysis showed that the IL-8 gene was actively transcribed in noninduced cells; upon stimulation
with LPS, the rate of IL-8 transcription was increased about 4-fold. A single mature mRNA species was detected by primer extension
analysis. The half-life of IL-8 mRNA transcripts in aveolar macrophages was approximately 2 h and did not change after LPS
stimulation. The ability of LPS to induce IL-8 expression was suppressed by recombinant human IL-4 and dexamethasone in a
concentration-dependent manner. These observations indicate that the expression of IL-8 is an early event in the sequelae
to bacterial infection in the lung.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8276881</pmid><doi>10.1016/s0021-9258(17)42316-7</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1994-01, Vol.269 (1), p.77-85 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16802517 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Analysis of the immune response. Humoral and cellular immunity Animals Base Sequence Biological and medical sciences Cloning, Molecular Dexamethasone - pharmacology DNA, Complementary Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Expression Regulation - drug effects Humans Immunobiology Interleukin-4 - pharmacology Interleukin-8 - biosynthesis Interleukin-8 - genetics Lipopolysaccharides - pharmacology Lymphokines, interleukins ( function, expression) Macrophages, Alveolar - metabolism Molecular Sequence Data Regulatory factors and their cellular receptors Sequence Homology, Amino Acid Swine Transcription, Genetic |
| title | Regulation of interleukin-8 expression in porcine alveolar macrophages by bacterial lipopolysaccharide |
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