Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene
The recA gene from the bacterium Xanthomonas oryzae pv. oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of Escherichia coli recA − mutants. The Xoo recA was localized to a 1.3-kb Sau3AI- XhoI fragment and, when cloned into pBR322, specifies increased m...
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| Veröffentlicht in: | Gene 1993-09, Vol.132 (1), p.113-118 |
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| creator | Rabibhadana, Siritida Chamnongpol, Sangpen Trempy, Janine E. Ambulos, Nicholas P. Mongkolsuk, Skorn |
| description | The
recA gene from the bacterium
Xanthomonas oryzae pv.
oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of
Escherichia coli recA
− mutants. The
Xoo recA was localized to a 1.3-kb
Sau3AI-
XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to
E. coli recA mutants and allows λ
red
−
gam
− to plaque on an
E. coli recA
− host. An
E. coli recA
− strain harboring a plasmid containing the
Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-
E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several
Xanthomonas pathovars using an anti-
E. coli RecA antibody. Furthermore, the cloned
Xoo recA was shown to hybridize to genomic DNA from various
Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the
Xoo recA gene. |
| doi_str_mv | 10.1016/0378-1119(93)90522-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16802493</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0378111993905225</els_id><sourcerecordid>16802493</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-60576b8510731ce88dea8df7316b4456bb517ef2a848fbae677949071dc9954e3</originalsourceid><addsrcrecordid>eNp9kE2LFDEQhoMo6-zqP1DMQUQPrUnno5OLsCyrLix4WBe8hep09U60JxmTHnH99Zt2hjlal6Kop16Kh5AXnL3njOsPTHSm4Zzbt1a8s0y1baMekRU3nW0YE-YxWR2Rp-S0lB-sllLtCTkxkmkmxIrcXJU0wRxSpBAHin-2GUtZxhDpZfFrzMGvA1CfpkDTSIF-hziv0yZFKDTl-7-ANKM_b6bwE-kdRnxGnowwFXx-6Gfk9tPlt4svzfXXz1cX59eNl5LPjWaq071RnHWCezRmQDDDWAfdS6l03yve4diCkWbsAXXXWWlZxwdvrZIozsibfe42p187LLPbhOJxmiBi2hXHtWGttKKCcg_6nErJOLptDhvI944zt7h0iyi3iHJWuH8unapnLw_5u36Dw_HoIK_uXx_2UDxMY4boQzliolPMiCXm1R4bITm4yxW5vWkZF4xLw7VqK_FxT2C19TtgdsUHjB6HUM3Obkjh_58-AEtbl9g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16802493</pqid></control><display><type>article</type><title>Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Rabibhadana, Siritida ; Chamnongpol, Sangpen ; Trempy, Janine E. ; Ambulos, Nicholas P. ; Mongkolsuk, Skorn</creator><creatorcontrib>Rabibhadana, Siritida ; Chamnongpol, Sangpen ; Trempy, Janine E. ; Ambulos, Nicholas P. ; Mongkolsuk, Skorn</creatorcontrib><description>The
recA gene from the bacterium
Xanthomonas oryzae pv.
oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of
Escherichia coli recA
− mutants. The
Xoo recA was localized to a 1.3-kb
Sau3AI-
XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to
E. coli recA mutants and allows λ
red
−
gam
− to plaque on an
E. coli recA
− host. An
E. coli recA
− strain harboring a plasmid containing the
Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-
E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several
Xanthomonas pathovars using an anti-
E. coli RecA antibody. Furthermore, the cloned
Xoo recA was shown to hybridize to genomic DNA from various
Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the
Xoo recA gene.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(93)90522-5</identifier><identifier>PMID: 8406033</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacteriophage lambda - physiology ; Biological and medical sciences ; Blotting, Southern ; Blotting, Western ; cloning ; Cloning, Molecular ; DNA repair ; DNA-binding proteins ; Escherichia coli ; Escherichia coli - drug effects ; Fundamental and applied biological sciences. Psychology ; Gene expression ; genetic complementation ; Genetic Complementation Test ; genetic transformation ; Methyl Methanesulfonate - pharmacology ; Mitomycin - pharmacology ; Molecular and cellular biology ; Molecular genetics ; mutagen resistance ; mutants ; Rec A Recombinases - genetics ; Rec A Recombinases - isolation & purification ; Rec A Recombinases - metabolism ; RecA ; RecA antigenically related protein ; reca gene ; Recombinant DNA ; rice pathogen ; Species Specificity ; structural genes ; Viral Plaque Assay ; Xanthomonas ; Xanthomonas - genetics ; Xanthomonas oryzae ; Xanthomonas oryzae pv. oryzae</subject><ispartof>Gene, 1993-09, Vol.132 (1), p.113-118</ispartof><rights>1993</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-60576b8510731ce88dea8df7316b4456bb517ef2a848fbae677949071dc9954e3</citedby><cites>FETCH-LOGICAL-c441t-60576b8510731ce88dea8df7316b4456bb517ef2a848fbae677949071dc9954e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(93)90522-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3750835$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8406033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rabibhadana, Siritida</creatorcontrib><creatorcontrib>Chamnongpol, Sangpen</creatorcontrib><creatorcontrib>Trempy, Janine E.</creatorcontrib><creatorcontrib>Ambulos, Nicholas P.</creatorcontrib><creatorcontrib>Mongkolsuk, Skorn</creatorcontrib><title>Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene</title><title>Gene</title><addtitle>Gene</addtitle><description>The
recA gene from the bacterium
Xanthomonas oryzae pv.
oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of
Escherichia coli recA
− mutants. The
Xoo recA was localized to a 1.3-kb
Sau3AI-
XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to
E. coli recA mutants and allows λ
red
−
gam
− to plaque on an
E. coli recA
− host. An
E. coli recA
− strain harboring a plasmid containing the
Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-
E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several
Xanthomonas pathovars using an anti-
E. coli RecA antibody. Furthermore, the cloned
Xoo recA was shown to hybridize to genomic DNA from various
Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the
Xoo recA gene.</description><subject>Bacteriophage lambda - physiology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>DNA repair</subject><subject>DNA-binding proteins</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>genetic complementation</subject><subject>Genetic Complementation Test</subject><subject>genetic transformation</subject><subject>Methyl Methanesulfonate - pharmacology</subject><subject>Mitomycin - pharmacology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>mutagen resistance</subject><subject>mutants</subject><subject>Rec A Recombinases - genetics</subject><subject>Rec A Recombinases - isolation & purification</subject><subject>Rec A Recombinases - metabolism</subject><subject>RecA</subject><subject>RecA antigenically related protein</subject><subject>reca gene</subject><subject>Recombinant DNA</subject><subject>rice pathogen</subject><subject>Species Specificity</subject><subject>structural genes</subject><subject>Viral Plaque Assay</subject><subject>Xanthomonas</subject><subject>Xanthomonas - genetics</subject><subject>Xanthomonas oryzae</subject><subject>Xanthomonas oryzae pv. oryzae</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo6-zqP1DMQUQPrUnno5OLsCyrLix4WBe8hep09U60JxmTHnH99Zt2hjlal6Kop16Kh5AXnL3njOsPTHSm4Zzbt1a8s0y1baMekRU3nW0YE-YxWR2Rp-S0lB-sllLtCTkxkmkmxIrcXJU0wRxSpBAHin-2GUtZxhDpZfFrzMGvA1CfpkDTSIF-hziv0yZFKDTl-7-ANKM_b6bwE-kdRnxGnowwFXx-6Gfk9tPlt4svzfXXz1cX59eNl5LPjWaq071RnHWCezRmQDDDWAfdS6l03yve4diCkWbsAXXXWWlZxwdvrZIozsibfe42p187LLPbhOJxmiBi2hXHtWGttKKCcg_6nErJOLptDhvI944zt7h0iyi3iHJWuH8unapnLw_5u36Dw_HoIK_uXx_2UDxMY4boQzliolPMiCXm1R4bITm4yxW5vWkZF4xLw7VqK_FxT2C19TtgdsUHjB6HUM3Obkjh_58-AEtbl9g</recordid><startdate>19930930</startdate><enddate>19930930</enddate><creator>Rabibhadana, Siritida</creator><creator>Chamnongpol, Sangpen</creator><creator>Trempy, Janine E.</creator><creator>Ambulos, Nicholas P.</creator><creator>Mongkolsuk, Skorn</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19930930</creationdate><title>Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene</title><author>Rabibhadana, Siritida ; Chamnongpol, Sangpen ; Trempy, Janine E. ; Ambulos, Nicholas P. ; Mongkolsuk, Skorn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-60576b8510731ce88dea8df7316b4456bb517ef2a848fbae677949071dc9954e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteriophage lambda - physiology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>DNA repair</topic><topic>DNA-binding proteins</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>genetic complementation</topic><topic>Genetic Complementation Test</topic><topic>genetic transformation</topic><topic>Methyl Methanesulfonate - pharmacology</topic><topic>Mitomycin - pharmacology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>mutagen resistance</topic><topic>mutants</topic><topic>Rec A Recombinases - genetics</topic><topic>Rec A Recombinases - isolation & purification</topic><topic>Rec A Recombinases - metabolism</topic><topic>RecA</topic><topic>RecA antigenically related protein</topic><topic>reca gene</topic><topic>Recombinant DNA</topic><topic>rice pathogen</topic><topic>Species Specificity</topic><topic>structural genes</topic><topic>Viral Plaque Assay</topic><topic>Xanthomonas</topic><topic>Xanthomonas - genetics</topic><topic>Xanthomonas oryzae</topic><topic>Xanthomonas oryzae pv. oryzae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rabibhadana, Siritida</creatorcontrib><creatorcontrib>Chamnongpol, Sangpen</creatorcontrib><creatorcontrib>Trempy, Janine E.</creatorcontrib><creatorcontrib>Ambulos, Nicholas P.</creatorcontrib><creatorcontrib>Mongkolsuk, Skorn</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rabibhadana, Siritida</au><au>Chamnongpol, Sangpen</au><au>Trempy, Janine E.</au><au>Ambulos, Nicholas P.</au><au>Mongkolsuk, Skorn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1993-09-30</date><risdate>1993</risdate><volume>132</volume><issue>1</issue><spage>113</spage><epage>118</epage><pages>113-118</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The
recA gene from the bacterium
Xanthomonas oryzae pv.
oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of
Escherichia coli recA
− mutants. The
Xoo recA was localized to a 1.3-kb
Sau3AI-
XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to
E. coli recA mutants and allows λ
red
−
gam
− to plaque on an
E. coli recA
− host. An
E. coli recA
− strain harboring a plasmid containing the
Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-
E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several
Xanthomonas pathovars using an anti-
E. coli RecA antibody. Furthermore, the cloned
Xoo recA was shown to hybridize to genomic DNA from various
Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the
Xoo recA gene.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8406033</pmid><doi>10.1016/0378-1119(93)90522-5</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1993-09, Vol.132 (1), p.113-118 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16802493 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacteriophage lambda - physiology Biological and medical sciences Blotting, Southern Blotting, Western cloning Cloning, Molecular DNA repair DNA-binding proteins Escherichia coli Escherichia coli - drug effects Fundamental and applied biological sciences. Psychology Gene expression genetic complementation Genetic Complementation Test genetic transformation Methyl Methanesulfonate - pharmacology Mitomycin - pharmacology Molecular and cellular biology Molecular genetics mutagen resistance mutants Rec A Recombinases - genetics Rec A Recombinases - isolation & purification Rec A Recombinases - metabolism RecA RecA antigenically related protein reca gene Recombinant DNA rice pathogen Species Specificity structural genes Viral Plaque Assay Xanthomonas Xanthomonas - genetics Xanthomonas oryzae Xanthomonas oryzae pv. oryzae |
| title | Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene |
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