Quantifying vitamin K-dependent holoprotein compaction caused by differential γ-carboxylation using high-pressure size exclusion chromatography

This study uses high-pressure size exclusion chromatography (HPSEC) to quantify divalent metal ion (X2+)-induced compaction found in vitamin K-dependent (VKD) proteins. Multiple X2+ binding sites formed by the presence of up to 12 γ-carboxyglutamic acid (Gla) residues are present in plasma-derived F...

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Veröffentlicht in:	Analytical biochemistry 2015-06, Vol.479, p.6-14
Hauptverfasser:	Vanderslice, Nicholas C., Messer, Amanda S., Vadivel, Kanagasabai, Bajaj, S. Paul, Phillips, Martin, Fatemi, Mostafa, Xu, Weijie, Velander, William H.
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Carboxyglutamic Acid - chemistry Binding Sites Calcium - metabolism Cations, Divalent - metabolism Chromatography, Gel - methods Factor IX Factor IX - chemistry Factor IX - metabolism High-pressure size exclusion chromatography Humans Magnesium - metabolism Models, Molecular Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - metabolism Vitamin K - metabolism Vitamin K-dependent protein γ-Carboxylation
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