Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates w...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Folia parasitologica 2015-01, Vol.62, p.1
Hauptverfasser: Vieira, Patricia Manuela, Mederle, Narcisa, Lobo, Maria Luisa, Imre, Kalman, Mederle, Ovidiu, Xiao, Lihua, Darabus, Gheorghe, Matos, Olga
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 1
container_title Folia parasitologica
container_volume 62
creator Vieira, Patricia Manuela
Mederle, Narcisa
Lobo, Maria Luisa
Imre, Kalman
Mederle, Ovidiu
Xiao, Lihua
Darabus, Gheorghe
Matos, Olga
description To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed.
doi_str_mv 10.14411/fp.2015.002
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1680208948</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3783235881</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-7828196b7c685cf3f81e9f0cb7c2aa738cd7fbaa57c07551b87bf314922f84f13</originalsourceid><addsrcrecordid>eNpdkEtLxDAUhYMozji6cy0FNwp2vGnz6lIGXzAiiC4lpGmCGdqmJi04_96OM7pwdeHwncPlQ-gUwxwTgvG17eYZYDoHyPbQFAvIU0YY3UdTGOOUMpFP0FGMKwBCOcAhmmS0YEAJm6L3J18bPdQqJPpDBaV7E1xUvfNt4m2yCOuu97HzwVVuaJKLm85p33S1-VKXiWvHkqurYNpEtVWiVd_XZhO_-Ea1Th2jA6vqaE52d4be7m5fFw_p8vn-cXGzTHVOeZ9ykQlcsJJrJqi2uRXYFBb0GGRK8VzoittSKco1cEpxKXhpc0yKLLOCWJzP0MV2twv-czCxl42L2tS1ao0fosRMQAaiIGJEz_-hKz-EdvxOYg6UEkpFMVJXW0oHH2MwVnbBNSqsJQb5o13aTm60y1H7iJ_tRoeyMdUf_Os5_waaqX0c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1705545589</pqid></control><display><type>article</type><title>Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania</title><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Vieira, Patricia Manuela ; Mederle, Narcisa ; Lobo, Maria Luisa ; Imre, Kalman ; Mederle, Ovidiu ; Xiao, Lihua ; Darabus, Gheorghe ; Matos, Olga</creator><creatorcontrib>Vieira, Patricia Manuela ; Mederle, Narcisa ; Lobo, Maria Luisa ; Imre, Kalman ; Mederle, Ovidiu ; Xiao, Lihua ; Darabus, Gheorghe ; Matos, Olga</creatorcontrib><description>To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed.</description><identifier>ISSN: 0015-5683</identifier><identifier>EISSN: 1803-6465</identifier><identifier>DOI: 10.14411/fp.2015.002</identifier><identifier>PMID: 25960546</identifier><language>eng</language><publisher>Czech Republic: Parazitologický ústav, Akademie ved Ceské republiky</publisher><ispartof>Folia parasitologica, 2015-01, Vol.62, p.1</ispartof><rights>Copyright Parazitologický ústav, Akademie ved Ceské republiky 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-7828196b7c685cf3f81e9f0cb7c2aa738cd7fbaa57c07551b87bf314922f84f13</citedby><cites>FETCH-LOGICAL-c357t-7828196b7c685cf3f81e9f0cb7c2aa738cd7fbaa57c07551b87bf314922f84f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25960546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vieira, Patricia Manuela</creatorcontrib><creatorcontrib>Mederle, Narcisa</creatorcontrib><creatorcontrib>Lobo, Maria Luisa</creatorcontrib><creatorcontrib>Imre, Kalman</creatorcontrib><creatorcontrib>Mederle, Ovidiu</creatorcontrib><creatorcontrib>Xiao, Lihua</creatorcontrib><creatorcontrib>Darabus, Gheorghe</creatorcontrib><creatorcontrib>Matos, Olga</creatorcontrib><title>Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania</title><title>Folia parasitologica</title><addtitle>Folia Parasitol (Praha)</addtitle><description>To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed.</description><issn>0015-5683</issn><issn>1803-6465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkEtLxDAUhYMozji6cy0FNwp2vGnz6lIGXzAiiC4lpGmCGdqmJi04_96OM7pwdeHwncPlQ-gUwxwTgvG17eYZYDoHyPbQFAvIU0YY3UdTGOOUMpFP0FGMKwBCOcAhmmS0YEAJm6L3J18bPdQqJPpDBaV7E1xUvfNt4m2yCOuu97HzwVVuaJKLm85p33S1-VKXiWvHkqurYNpEtVWiVd_XZhO_-Ea1Th2jA6vqaE52d4be7m5fFw_p8vn-cXGzTHVOeZ9ykQlcsJJrJqi2uRXYFBb0GGRK8VzoittSKco1cEpxKXhpc0yKLLOCWJzP0MV2twv-czCxl42L2tS1ao0fosRMQAaiIGJEz_-hKz-EdvxOYg6UEkpFMVJXW0oHH2MwVnbBNSqsJQb5o13aTm60y1H7iJ_tRoeyMdUf_Os5_waaqX0c</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Vieira, Patricia Manuela</creator><creator>Mederle, Narcisa</creator><creator>Lobo, Maria Luisa</creator><creator>Imre, Kalman</creator><creator>Mederle, Ovidiu</creator><creator>Xiao, Lihua</creator><creator>Darabus, Gheorghe</creator><creator>Matos, Olga</creator><general>Parazitologický ústav, Akademie ved Ceské republiky</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20150101</creationdate><title>Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania</title><author>Vieira, Patricia Manuela ; Mederle, Narcisa ; Lobo, Maria Luisa ; Imre, Kalman ; Mederle, Ovidiu ; Xiao, Lihua ; Darabus, Gheorghe ; Matos, Olga</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-7828196b7c685cf3f81e9f0cb7c2aa738cd7fbaa57c07551b87bf314922f84f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vieira, Patricia Manuela</creatorcontrib><creatorcontrib>Mederle, Narcisa</creatorcontrib><creatorcontrib>Lobo, Maria Luisa</creatorcontrib><creatorcontrib>Imre, Kalman</creatorcontrib><creatorcontrib>Mederle, Ovidiu</creatorcontrib><creatorcontrib>Xiao, Lihua</creatorcontrib><creatorcontrib>Darabus, Gheorghe</creatorcontrib><creatorcontrib>Matos, Olga</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Folia parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vieira, Patricia Manuela</au><au>Mederle, Narcisa</au><au>Lobo, Maria Luisa</au><au>Imre, Kalman</au><au>Mederle, Ovidiu</au><au>Xiao, Lihua</au><au>Darabus, Gheorghe</au><au>Matos, Olga</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania</atitle><jtitle>Folia parasitologica</jtitle><addtitle>Folia Parasitol (Praha)</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>62</volume><spage>1</spage><pages>1-</pages><issn>0015-5683</issn><eissn>1803-6465</eissn><abstract>To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed.</abstract><cop>Czech Republic</cop><pub>Parazitologický ústav, Akademie ved Ceské republiky</pub><pmid>25960546</pmid><doi>10.14411/fp.2015.002</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0015-5683
ispartof Folia parasitologica, 2015-01, Vol.62, p.1
issn 0015-5683
1803-6465
language eng
recordid cdi_proquest_miscellaneous_1680208948
source EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
title Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T14%3A22%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20characterisation%20of%20Cryptosporidium%20(Apicomplexa)%20in%20children%20and%20cattle%20in%20Romania&rft.jtitle=Folia%20parasitologica&rft.au=Vieira,%20Patricia%20Manuela&rft.date=2015-01-01&rft.volume=62&rft.spage=1&rft.pages=1-&rft.issn=0015-5683&rft.eissn=1803-6465&rft_id=info:doi/10.14411/fp.2015.002&rft_dat=%3Cproquest_cross%3E3783235881%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1705545589&rft_id=info:pmid/25960546&rfr_iscdi=true