Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation
An Agrobacterium -mediated plant transformation system was developed for the generation of transgenic banana ( Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium . Two or more cycles of...
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Veröffentlicht in: | Bio/Technology 1995-05, Vol.13 (5), p.486-492 |
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creator | May, G.D. (Texas AandM University, Houston, TX.) Afza, R Mason, H.S Wiecko, A Novak, F.J Arntzen, C.J |
description | An
Agrobacterium
-mediated plant transformation system was developed for the generation of transgenic banana (
Musa
spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with
Agrobacterium
. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations. |
doi_str_mv | 10.1038/nbt0595-486 |
format | Article |
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Agrobacterium
-mediated plant transformation system was developed for the generation of transgenic banana (
Musa
spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with
Agrobacterium
. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.</description><identifier>ISSN: 0733-222X</identifier><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0595-486</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; AGROBACTERIUM ; BETA-GLUCURONIDASE ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; DRUGS ; FOSFOTRASFERASA ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE TRANSFER ; GENES ; Genetic engineering ; Genetic technics ; GENETIC TRANSFORMATION ; GLICOSIDASAS ; GLYCOSIDASE ; GLYCOSIDASES ; gusa gene ; HISTOENZYMOLOGY ; IN VITRO REGENERATION ; KANAMICINA ; KANAMYCIN ; KANAMYCINE ; Life Sciences ; MEDICAMENT ; MEDICAMENTOS ; Methods. Procedures. Technologies ; MICROPROPAGACION ; MICROPROPAGATION ; Musa acuminata ; neomycin phosphotransferase ii ; nptii gene ; PHOSPHOTRANSFERASE ; PHOSPHOTRANSFERASES ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; PLASMID VECTORS ; REGENERACION IN VITRO ; REGENERATION IN VITRO ; REPORTER GENES ; RESISTANCE AUX PRODUITS CHIMIQUES ; RESISTANCE TO CHEMICALS ; RESISTENCIA A PRODUCTOS QUIMICOS ; SHOOTS ; STEMS ; TALLO ; TIGE ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Transgenic animals and transgenic plants ; TRANSGENIC PLANTS</subject><ispartof>Bio/Technology, 1995-05, Vol.13 (5), p.486-492</ispartof><rights>Nature Publishing Company 1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-caa6b14169daeb56e088da9fff58c3228f8571506a7f7fad3fc1d3d630c80ded3</citedby><cites>FETCH-LOGICAL-c409t-caa6b14169daeb56e088da9fff58c3228f8571506a7f7fad3fc1d3d630c80ded3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3511330$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>May, G.D. (Texas AandM University, Houston, TX.)</creatorcontrib><creatorcontrib>Afza, R</creatorcontrib><creatorcontrib>Mason, H.S</creatorcontrib><creatorcontrib>Wiecko, A</creatorcontrib><creatorcontrib>Novak, F.J</creatorcontrib><creatorcontrib>Arntzen, C.J</creatorcontrib><title>Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><description>An
Agrobacterium
-mediated plant transformation system was developed for the generation of transgenic banana (
Musa
spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with
Agrobacterium
. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.</description><subject>Agriculture</subject><subject>AGROBACTERIUM</subject><subject>BETA-GLUCURONIDASE</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>DRUGS</subject><subject>FOSFOTRASFERASA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE TRANSFER</subject><subject>GENES</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>GENETIC TRANSFORMATION</subject><subject>GLICOSIDASAS</subject><subject>GLYCOSIDASE</subject><subject>GLYCOSIDASES</subject><subject>gusa gene</subject><subject>HISTOENZYMOLOGY</subject><subject>IN VITRO REGENERATION</subject><subject>KANAMICINA</subject><subject>KANAMYCIN</subject><subject>KANAMYCINE</subject><subject>Life Sciences</subject><subject>MEDICAMENT</subject><subject>MEDICAMENTOS</subject><subject>Methods. Procedures. Technologies</subject><subject>MICROPROPAGACION</subject><subject>MICROPROPAGATION</subject><subject>Musa acuminata</subject><subject>neomycin phosphotransferase ii</subject><subject>nptii gene</subject><subject>PHOSPHOTRANSFERASE</subject><subject>PHOSPHOTRANSFERASES</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>PLASMID VECTORS</subject><subject>REGENERACION IN VITRO</subject><subject>REGENERATION IN VITRO</subject><subject>REPORTER GENES</subject><subject>RESISTANCE AUX PRODUITS CHIMIQUES</subject><subject>RESISTANCE TO CHEMICALS</subject><subject>RESISTENCIA A PRODUCTOS QUIMICOS</subject><subject>SHOOTS</subject><subject>STEMS</subject><subject>TALLO</subject><subject>TIGE</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Transgenic animals and transgenic plants</subject><subject>TRANSGENIC PLANTS</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp1kc9LHDEUx4ModLt66s3TFIq06NRkMslkjiKtCpYerNBbeJMfS5adZJtkBP97U2dZvEgO75DP--S9bxD6RPB3gqm49EPGrGd1K_gBWjSUkppy0R6iBe4orZum-fsBfUxpjXHb8aZdILgx3kTILvgq2CpH8GllvFPVAL6c6uuvKUEFahqdhwzfqu0GfE7Vk4PqahXDACqb6KaxHo12kI2eJTbE8VV7jI4sbJI52dUlevz548_1bX3_--bu-uq-Vi3uc60A-EBawnsNZmDcYCE09NZaJhRtGmEF6wjDHDrbWdDUKqKp5hQrgbXRdInOZu82hn-TSVmOLimzKeOaMCVJuMCY8b6A5zOoYkgpGiu30Y0QnyXB8n-MchejLDEW-stOC0nBxpbdlEv7FsoIoRQX7GLGUrnxKxPlOkzRl4XfsX6e8ZLpFM1e9-YDC3M6MxaChFUsrz4-9Kztmq6jL16dlqE</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>May, G.D. (Texas AandM University, Houston, TX.)</creator><creator>Afza, R</creator><creator>Mason, H.S</creator><creator>Wiecko, A</creator><creator>Novak, F.J</creator><creator>Arntzen, C.J</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19950501</creationdate><title>Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation</title><author>May, G.D. (Texas AandM University, Houston, TX.) ; Afza, R ; Mason, H.S ; Wiecko, A ; Novak, F.J ; Arntzen, C.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-caa6b14169daeb56e088da9fff58c3228f8571506a7f7fad3fc1d3d630c80ded3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Agriculture</topic><topic>AGROBACTERIUM</topic><topic>BETA-GLUCURONIDASE</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>DRUGS</topic><topic>FOSFOTRASFERASA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE TRANSFER</topic><topic>GENES</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>GENETIC TRANSFORMATION</topic><topic>GLICOSIDASAS</topic><topic>GLYCOSIDASE</topic><topic>GLYCOSIDASES</topic><topic>gusa gene</topic><topic>HISTOENZYMOLOGY</topic><topic>IN VITRO REGENERATION</topic><topic>KANAMICINA</topic><topic>KANAMYCIN</topic><topic>KANAMYCINE</topic><topic>Life Sciences</topic><topic>MEDICAMENT</topic><topic>MEDICAMENTOS</topic><topic>Methods. Procedures. Technologies</topic><topic>MICROPROPAGACION</topic><topic>MICROPROPAGATION</topic><topic>Musa acuminata</topic><topic>neomycin phosphotransferase ii</topic><topic>nptii gene</topic><topic>PHOSPHOTRANSFERASE</topic><topic>PHOSPHOTRANSFERASES</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>PLASMID VECTORS</topic><topic>REGENERACION IN VITRO</topic><topic>REGENERATION IN VITRO</topic><topic>REPORTER GENES</topic><topic>RESISTANCE AUX PRODUITS CHIMIQUES</topic><topic>RESISTANCE TO CHEMICALS</topic><topic>RESISTENCIA A PRODUCTOS QUIMICOS</topic><topic>SHOOTS</topic><topic>STEMS</topic><topic>TALLO</topic><topic>TIGE</topic><topic>TRANSFERENCIA DE GENES</topic><topic>TRANSFERT DE GENE</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Transgenic animals and transgenic plants</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>May, G.D. (Texas AandM University, Houston, TX.)</creatorcontrib><creatorcontrib>Afza, R</creatorcontrib><creatorcontrib>Mason, H.S</creatorcontrib><creatorcontrib>Wiecko, A</creatorcontrib><creatorcontrib>Novak, F.J</creatorcontrib><creatorcontrib>Arntzen, C.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>May, G.D. (Texas AandM University, Houston, TX.)</au><au>Afza, R</au><au>Mason, H.S</au><au>Wiecko, A</au><au>Novak, F.J</au><au>Arntzen, C.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><date>1995-05-01</date><risdate>1995</risdate><volume>13</volume><issue>5</issue><spage>486</spage><epage>492</epage><pages>486-492</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>An
Agrobacterium
-mediated plant transformation system was developed for the generation of transgenic banana (
Musa
spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with
Agrobacterium
. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/nbt0595-486</doi><tpages>7</tpages></addata></record> |
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ispartof | Bio/Technology, 1995-05, Vol.13 (5), p.486-492 |
issn | 0733-222X 1087-0156 2331-3684 1546-1696 |
language | eng |
recordid | cdi_proquest_miscellaneous_16800569 |
source | Nature; Alma/SFX Local Collection |
subjects | Agriculture AGROBACTERIUM BETA-GLUCURONIDASE Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology DRUGS FOSFOTRASFERASA Fundamental and applied biological sciences. Psychology GENE GENE TRANSFER GENES Genetic engineering Genetic technics GENETIC TRANSFORMATION GLICOSIDASAS GLYCOSIDASE GLYCOSIDASES gusa gene HISTOENZYMOLOGY IN VITRO REGENERATION KANAMICINA KANAMYCIN KANAMYCINE Life Sciences MEDICAMENT MEDICAMENTOS Methods. Procedures. Technologies MICROPROPAGACION MICROPROPAGATION Musa acuminata neomycin phosphotransferase ii nptii gene PHOSPHOTRANSFERASE PHOSPHOTRANSFERASES PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE PLASMID VECTORS REGENERACION IN VITRO REGENERATION IN VITRO REPORTER GENES RESISTANCE AUX PRODUITS CHIMIQUES RESISTANCE TO CHEMICALS RESISTENCIA A PRODUCTOS QUIMICOS SHOOTS STEMS TALLO TIGE TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transgenic animals and transgenic plants TRANSGENIC PLANTS |
title | Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation |
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