Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation

An Agrobacterium -mediated plant transformation system was developed for the generation of transgenic banana ( Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium . Two or more cycles of...

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Veröffentlicht in:Bio/Technology 1995-05, Vol.13 (5), p.486-492
Hauptverfasser: May, G.D. (Texas AandM University, Houston, TX.), Afza, R, Mason, H.S, Wiecko, A, Novak, F.J, Arntzen, C.J
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container_end_page 492
container_issue 5
container_start_page 486
container_title Bio/Technology
container_volume 13
creator May, G.D. (Texas AandM University, Houston, TX.)
Afza, R
Mason, H.S
Wiecko, A
Novak, F.J
Arntzen, C.J
description An Agrobacterium -mediated plant transformation system was developed for the generation of transgenic banana ( Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium . Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.
doi_str_mv 10.1038/nbt0595-486
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(Texas AandM University, Houston, TX.) ; Afza, R ; Mason, H.S ; Wiecko, A ; Novak, F.J ; Arntzen, C.J</creator><creatorcontrib>May, G.D. (Texas AandM University, Houston, TX.) ; Afza, R ; Mason, H.S ; Wiecko, A ; Novak, F.J ; Arntzen, C.J</creatorcontrib><description>An Agrobacterium -mediated plant transformation system was developed for the generation of transgenic banana ( Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium . Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. 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fulltext fulltext
identifier ISSN: 0733-222X
ispartof Bio/Technology, 1995-05, Vol.13 (5), p.486-492
issn 0733-222X
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2331-3684
1546-1696
language eng
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source Nature; Alma/SFX Local Collection
subjects Agriculture
AGROBACTERIUM
BETA-GLUCURONIDASE
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
DRUGS
FOSFOTRASFERASA
Fundamental and applied biological sciences. Psychology
GENE
GENE TRANSFER
GENES
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
GLICOSIDASAS
GLYCOSIDASE
GLYCOSIDASES
gusa gene
HISTOENZYMOLOGY
IN VITRO REGENERATION
KANAMICINA
KANAMYCIN
KANAMYCINE
Life Sciences
MEDICAMENT
MEDICAMENTOS
Methods. Procedures. Technologies
MICROPROPAGACION
MICROPROPAGATION
Musa acuminata
neomycin phosphotransferase ii
nptii gene
PHOSPHOTRANSFERASE
PHOSPHOTRANSFERASES
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
PLASMID VECTORS
REGENERACION IN VITRO
REGENERATION IN VITRO
REPORTER GENES
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTANCE TO CHEMICALS
RESISTENCIA A PRODUCTOS QUIMICOS
SHOOTS
STEMS
TALLO
TIGE
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transgenic animals and transgenic plants
TRANSGENIC PLANTS
title Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation
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