INITIATION AND TERMINATION OF DNA REPLICATION OF PLASMID pKYM VIA A ROLLING-CIRCLE MECHANISM

The replication origin of plasmid pKYM, which replicates via a rolling-circle mechanism, is composed of three regions with the following characteristics. 1) The core origin, which is essential for initiation of DNA replication, consists of 39-base pairs. The purified Rep protein of pKYM (RepK) binds...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of general and applied microbiology 1994, Vol.40(5), pp.377-388
Hauptverfasser:	YASUKAWA, HIROO, OZAKI, EIJIRO, MORITO, SATOSHI, MASAMUNE, YUKITO
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Fundamental and applied biological sciences. Psychology Genetics Microbiology Mission oriented research
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	388
container_issue	5
container_start_page	377
container_title	Journal of general and applied microbiology
container_volume	40
creator	YASUKAWA, HIROO OZAKI, EIJIRO MORITO, SATOSHI MASAMUNE, YUKITO
description	The replication origin of plasmid pKYM, which replicates via a rolling-circle mechanism, is composed of three regions with the following characteristics. 1) The core origin, which is essential for initiation of DNA replication, consists of 39-base pairs. The purified Rep protein of pKYM (RepK) binds specifically to this 39-base-pair region. Within the 39 base-pair origin, there is a sequence of 22 base pairs that is sufficient for termination. 2) The initiation-enhancing region consists of a 98-base-pair-region downstream of the core origin. A host protein(s) binds to this region in the presence of RepK. 3) A 34-base-pair sequence upstream of the core origin is essential for the multiplication of pKYM. Multimers of the plasmid DNA were produced in the absence of this region. The region may enhance correct processing of the unit length plasmid DNA. A host protein binds to this region.
doi_str_mv	10.2323/jgam.40.377
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16795872</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16795872</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-5954c229550127e3deec23416b2b05cf3449cd07a6b92e0dfcd96f0be7d565af3</originalsourceid><addsrcrecordid>eNo9kF1PwjAUhhujiYhe-Qd2Ybwxw9OPrexyGQMat0EATUxMmtJ1CBkfrnDhv7cI4aYn7XnOk9MXoUcMHUIJfV0t1LrDoEM5v0ItTFnkd4Hya9QCIMTHJIRbdGftCoCGpMta6EsUYibimRgVXlz0vFk6yUVxuo_6Xq-IvUk6zkRyeRpn8TQXPW_39pl7HyL2HDHKMlEM_ERMkiz18jQZxoWY5vfoplK1NQ_n2kbv_XSWDP1sNHDGzNeMw94PooBpQqIgAEy4oaUxmlCGwzmZQ6ArylikS-AqnEfEQFnpMgormBteBmGgKtpGzyfvrtn-HIzdy_XSalPXamO2BytxyKOgy4kDX06gbrbWNqaSu2a5Vs2vxCCPCcpjgpKBdAk6-umsVVarumrURi_tZcSthaEbOqx3wlZ2rxbm0lfNfqlr86_E7nNH7flw9ktbf6tGmg39AzB8gKo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16795872</pqid></control><display><type>article</type><title>INITIATION AND TERMINATION OF DNA REPLICATION OF PLASMID pKYM VIA A ROLLING-CIRCLE MECHANISM</title><source>J-STAGE Free</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>YASUKAWA, HIROO ; OZAKI, EIJIRO ; MORITO, SATOSHI ; MASAMUNE, YUKITO</creator><creatorcontrib>YASUKAWA, HIROO ; OZAKI, EIJIRO ; MORITO, SATOSHI ; MASAMUNE, YUKITO</creatorcontrib><description>The replication origin of plasmid pKYM, which replicates via a rolling-circle mechanism, is composed of three regions with the following characteristics. 1) The core origin, which is essential for initiation of DNA replication, consists of 39-base pairs. The purified Rep protein of pKYM (RepK) binds specifically to this 39-base-pair region. Within the 39 base-pair origin, there is a sequence of 22 base pairs that is sufficient for termination. 2) The initiation-enhancing region consists of a 98-base-pair-region downstream of the core origin. A host protein(s) binds to this region in the presence of RepK. 3) A 34-base-pair sequence upstream of the core origin is essential for the multiplication of pKYM. Multimers of the plasmid DNA were produced in the absence of this region. The region may enhance correct processing of the unit length plasmid DNA. A host protein binds to this region.</description><identifier>ISSN: 0022-1260</identifier><identifier>EISSN: 1349-8037</identifier><identifier>DOI: 10.2323/jgam.40.377</identifier><identifier>CODEN: JGAMA9</identifier><language>eng</language><publisher>Tokyo: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</publisher><subject>Bacteriology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Genetics ; Microbiology ; Mission oriented research</subject><ispartof>The Journal of General and Applied Microbiology, 1994, Vol.40(5), pp.377-388</ispartof><rights>The Microbiology Research Foundation</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1877,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3441086$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>YASUKAWA, HIROO</creatorcontrib><creatorcontrib>OZAKI, EIJIRO</creatorcontrib><creatorcontrib>MORITO, SATOSHI</creatorcontrib><creatorcontrib>MASAMUNE, YUKITO</creatorcontrib><title>INITIATION AND TERMINATION OF DNA REPLICATION OF PLASMID pKYM VIA A ROLLING-CIRCLE MECHANISM</title><title>Journal of general and applied microbiology</title><addtitle>J. Gen. Appl. Microbiol.</addtitle><description>The replication origin of plasmid pKYM, which replicates via a rolling-circle mechanism, is composed of three regions with the following characteristics. 1) The core origin, which is essential for initiation of DNA replication, consists of 39-base pairs. The purified Rep protein of pKYM (RepK) binds specifically to this 39-base-pair region. Within the 39 base-pair origin, there is a sequence of 22 base pairs that is sufficient for termination. 2) The initiation-enhancing region consists of a 98-base-pair-region downstream of the core origin. A host protein(s) binds to this region in the presence of RepK. 3) A 34-base-pair sequence upstream of the core origin is essential for the multiplication of pKYM. Multimers of the plasmid DNA were produced in the absence of this region. The region may enhance correct processing of the unit length plasmid DNA. A host protein binds to this region.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><issn>0022-1260</issn><issn>1349-8037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNo9kF1PwjAUhhujiYhe-Qd2Ybwxw9OPrexyGQMat0EATUxMmtJ1CBkfrnDhv7cI4aYn7XnOk9MXoUcMHUIJfV0t1LrDoEM5v0ItTFnkd4Hya9QCIMTHJIRbdGftCoCGpMta6EsUYibimRgVXlz0vFk6yUVxuo_6Xq-IvUk6zkRyeRpn8TQXPW_39pl7HyL2HDHKMlEM_ERMkiz18jQZxoWY5vfoplK1NQ_n2kbv_XSWDP1sNHDGzNeMw94PooBpQqIgAEy4oaUxmlCGwzmZQ6ArylikS-AqnEfEQFnpMgormBteBmGgKtpGzyfvrtn-HIzdy_XSalPXamO2BytxyKOgy4kDX06gbrbWNqaSu2a5Vs2vxCCPCcpjgpKBdAk6-umsVVarumrURi_tZcSthaEbOqx3wlZ2rxbm0lfNfqlr86_E7nNH7flw9ktbf6tGmg39AzB8gKo</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>YASUKAWA, HIROO</creator><creator>OZAKI, EIJIRO</creator><creator>MORITO, SATOSHI</creator><creator>MASAMUNE, YUKITO</creator><general>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</general><general>Microbiology Research Foundation</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>1994</creationdate><title>INITIATION AND TERMINATION OF DNA REPLICATION OF PLASMID pKYM VIA A ROLLING-CIRCLE MECHANISM</title><author>YASUKAWA, HIROO ; OZAKI, EIJIRO ; MORITO, SATOSHI ; MASAMUNE, YUKITO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-5954c229550127e3deec23416b2b05cf3449cd07a6b92e0dfcd96f0be7d565af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YASUKAWA, HIROO</creatorcontrib><creatorcontrib>OZAKI, EIJIRO</creatorcontrib><creatorcontrib>MORITO, SATOSHI</creatorcontrib><creatorcontrib>MASAMUNE, YUKITO</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of general and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YASUKAWA, HIROO</au><au>OZAKI, EIJIRO</au><au>MORITO, SATOSHI</au><au>MASAMUNE, YUKITO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>INITIATION AND TERMINATION OF DNA REPLICATION OF PLASMID pKYM VIA A ROLLING-CIRCLE MECHANISM</atitle><jtitle>Journal of general and applied microbiology</jtitle><addtitle>J. Gen. Appl. Microbiol.</addtitle><date>1994</date><risdate>1994</risdate><volume>40</volume><issue>5</issue><spage>377</spage><epage>388</epage><pages>377-388</pages><issn>0022-1260</issn><eissn>1349-8037</eissn><coden>JGAMA9</coden><abstract>The replication origin of plasmid pKYM, which replicates via a rolling-circle mechanism, is composed of three regions with the following characteristics. 1) The core origin, which is essential for initiation of DNA replication, consists of 39-base pairs. The purified Rep protein of pKYM (RepK) binds specifically to this 39-base-pair region. Within the 39 base-pair origin, there is a sequence of 22 base pairs that is sufficient for termination. 2) The initiation-enhancing region consists of a 98-base-pair-region downstream of the core origin. A host protein(s) binds to this region in the presence of RepK. 3) A 34-base-pair sequence upstream of the core origin is essential for the multiplication of pKYM. Multimers of the plasmid DNA were produced in the absence of this region. The region may enhance correct processing of the unit length plasmid DNA. A host protein binds to this region.</abstract><cop>Tokyo</cop><pub>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</pub><doi>10.2323/jgam.40.377</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	ISSN: 0022-1260
ispartof	The Journal of General and Applied Microbiology, 1994, Vol.40(5), pp.377-388
issn	0022-1260 1349-8037
language	eng
recordid	cdi_proquest_miscellaneous_16795872
source	J-STAGE Free; EZB-FREE-00999 freely available EZB journals
subjects	Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Fundamental and applied biological sciences. Psychology Genetics Microbiology Mission oriented research
title	INITIATION AND TERMINATION OF DNA REPLICATION OF PLASMID pKYM VIA A ROLLING-CIRCLE MECHANISM
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T01%3A12%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=INITIATION%20AND%20TERMINATION%20OF%20DNA%20REPLICATION%20OF%20PLASMID%20pKYM%20VIA%20A%20ROLLING-CIRCLE%20MECHANISM&rft.jtitle=Journal%20of%20general%20and%20applied%20microbiology&rft.au=YASUKAWA,%20HIROO&rft.date=1994&rft.volume=40&rft.issue=5&rft.spage=377&rft.epage=388&rft.pages=377-388&rft.issn=0022-1260&rft.eissn=1349-8037&rft.coden=JGAMA9&rft_id=info:doi/10.2323/jgam.40.377&rft_dat=%3Cproquest_cross%3E16795872%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16795872&rft_id=info:pmid/&rfr_iscdi=true