Localization of chromosome scaffold by transmitted- and incident-light fluorescence microscopy
In order to demonstrate the chromosome scaffold the spermatocytes of a grasshopper were fixed in acetomethanol, treated with alkaline 2XSSC and stained with AgNO3 solution. The air-dried preparations were fluorochromed with acridine orange (AO) and excited with either transmitted or incident beam of...
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| Veröffentlicht in: | Current science (Bangalore) 1994-07, Vol.67 (2), p.109-112 |
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| container_title | Current science (Bangalore) |
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| creator | De, Deepesh N. Ghosh, Rekha |
| description | In order to demonstrate the chromosome scaffold the spermatocytes of a grasshopper were fixed in acetomethanol, treated with alkaline 2XSSC and stained with AgNO3 solution. The air-dried preparations were fluorochromed with acridine orange (AO) and excited with either transmitted or incident beam of UV for fluorescence microscopic study. Under transmitted-light the scaffold is very clearly visible as non-fluorescent dark silver deposition along the middle of the chromosomes. However, under incident-illumination the scaffold is invisible and only the fluorescence of the epichromatin is observed. This indicates that the scaffold is situated longitudinally in the centre of a solid cylinder of epichromatin made of DNA-histone. |
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The air-dried preparations were fluorochromed with acridine orange (AO) and excited with either transmitted or incident beam of UV for fluorescence microscopic study. Under transmitted-light the scaffold is very clearly visible as non-fluorescent dark silver deposition along the middle of the chromosomes. However, under incident-illumination the scaffold is invisible and only the fluorescence of the epichromatin is observed. This indicates that the scaffold is situated longitudinally in the centre of a solid cylinder of epichromatin made of DNA-histone.</description><identifier>ISSN: 0011-3891</identifier><language>eng</language><publisher>Current Science Association</publisher><subject>Acrididae ; Acridines ; Anaphase ; Chromatin ; Chromosomes ; Condensation ; DNA ; Fluorescence ; Microscopy ; Orthoptera ; RESEARCH COMMUNICATIONS ; Scaffolds ; Silver</subject><ispartof>Current science (Bangalore), 1994-07, Vol.67 (2), p.109-112</ispartof><rights>Copyright © 1994 Indian Academy of Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24096074$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24096074$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,782,786,805,58024,58257</link.rule.ids></links><search><creatorcontrib>De, Deepesh N.</creatorcontrib><creatorcontrib>Ghosh, Rekha</creatorcontrib><title>Localization of chromosome scaffold by transmitted- and incident-light fluorescence microscopy</title><title>Current science (Bangalore)</title><description>In order to demonstrate the chromosome scaffold the spermatocytes of a grasshopper were fixed in acetomethanol, treated with alkaline 2XSSC and stained with AgNO3 solution. The air-dried preparations were fluorochromed with acridine orange (AO) and excited with either transmitted or incident beam of UV for fluorescence microscopic study. Under transmitted-light the scaffold is very clearly visible as non-fluorescent dark silver deposition along the middle of the chromosomes. However, under incident-illumination the scaffold is invisible and only the fluorescence of the epichromatin is observed. This indicates that the scaffold is situated longitudinally in the centre of a solid cylinder of epichromatin made of DNA-histone.</description><subject>Acrididae</subject><subject>Acridines</subject><subject>Anaphase</subject><subject>Chromatin</subject><subject>Chromosomes</subject><subject>Condensation</subject><subject>DNA</subject><subject>Fluorescence</subject><subject>Microscopy</subject><subject>Orthoptera</subject><subject>RESEARCH COMMUNICATIONS</subject><subject>Scaffolds</subject><subject>Silver</subject><issn>0011-3891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNotjrtqwzAUQD200PTxCQVN3QyyZVn2WEJfEOiSueb66qpRkHVTSRnSr28gnc5yOJyraiVl09RqGJub6jbnvZStauW4qr42jBD8LxTPUbATuEu8cOaFREZwjoMV80mUBDEvvhSytYBohY_oLcVSB_-9K8KFIyfKSBFJLB4TZ-TD6b66dhAyPfzzrtq-vmzX7_Xm8-1j_byp91qZ2qBDMK3SioYOJEjZWwuttjQrGJwiNeOsYQYYSWNHSHLoW2OJepRjP6i76umSPST-OVIu0-LPLyFAJD7mqenN0GttzuLjRdznwmk6JL9AOk1td85I06k_LUBcsw</recordid><startdate>19940725</startdate><enddate>19940725</enddate><creator>De, Deepesh N.</creator><creator>Ghosh, Rekha</creator><general>Current Science Association</general><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19940725</creationdate><title>Localization of chromosome scaffold by transmitted- and incident-light fluorescence microscopy</title><author>De, Deepesh N. ; Ghosh, Rekha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j537-7cfca72353e84a0a006dda25deb3a8f3e3bcb5abaa9e5c4ece08627dee6c09683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acrididae</topic><topic>Acridines</topic><topic>Anaphase</topic><topic>Chromatin</topic><topic>Chromosomes</topic><topic>Condensation</topic><topic>DNA</topic><topic>Fluorescence</topic><topic>Microscopy</topic><topic>Orthoptera</topic><topic>RESEARCH COMMUNICATIONS</topic><topic>Scaffolds</topic><topic>Silver</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De, Deepesh N.</creatorcontrib><creatorcontrib>Ghosh, Rekha</creatorcontrib><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Current science (Bangalore)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De, Deepesh N.</au><au>Ghosh, Rekha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of chromosome scaffold by transmitted- and incident-light fluorescence microscopy</atitle><jtitle>Current science (Bangalore)</jtitle><date>1994-07-25</date><risdate>1994</risdate><volume>67</volume><issue>2</issue><spage>109</spage><epage>112</epage><pages>109-112</pages><issn>0011-3891</issn><abstract>In order to demonstrate the chromosome scaffold the spermatocytes of a grasshopper were fixed in acetomethanol, treated with alkaline 2XSSC and stained with AgNO3 solution. The air-dried preparations were fluorochromed with acridine orange (AO) and excited with either transmitted or incident beam of UV for fluorescence microscopic study. Under transmitted-light the scaffold is very clearly visible as non-fluorescent dark silver deposition along the middle of the chromosomes. However, under incident-illumination the scaffold is invisible and only the fluorescence of the epichromatin is observed. This indicates that the scaffold is situated longitudinally in the centre of a solid cylinder of epichromatin made of DNA-histone.</abstract><pub>Current Science Association</pub><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-3891 |
| ispartof | Current science (Bangalore), 1994-07, Vol.67 (2), p.109-112 |
| issn | 0011-3891 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16786557 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing |
| subjects | Acrididae Acridines Anaphase Chromatin Chromosomes Condensation DNA Fluorescence Microscopy Orthoptera RESEARCH COMMUNICATIONS Scaffolds Silver |
| title | Localization of chromosome scaffold by transmitted- and incident-light fluorescence microscopy |
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