Targeted metabolomics in cultured cells and tissues by mass spectrometry: Method development and validation

[Display omitted] •This study introduce a valid bioanalytical method for metabolic profiling.•The method validation and stability were studied carefully based on USFDA guidelines.•The targeted metabolomics method was tested on couple of biological systems cell lines and mouse tissue. Metabolomics is the identification and quantitation of small bio-molecules (metabolites) in biological samples under various environmental and genetic conditions. Mass spectrometry provides the unique opportunity for targeted identification and quantification of known metabolites by selective reaction monitoring (SRM). However, reproducibility of this approach depends on careful consideration of sample preparation, chemical classes, and stability of metabolites to be evaluated. Herein, we introduce and validate a targeted metabolite profiling workflow for cultured cells and tissues by liquid chromatography–triple quadrupole tandem mass spectrometry. The method requires a one-step extraction of water-soluble metabolites and targeted analysis of central metabolites that include glycolysis, amino acids, nucleotides, citric acid cycle, and the hexosamine biosynthetic pathway. The sensitivity, reproducibility and molecular stability of each targeted metabolite were assessed under experimental conditions. Quantitation of metabolites by peak area ratio was linear with a dilution over a 4 fold dynamic range with minimal deviation R2=0.98. Inter- and intra-day precision with cells and tissues had an average coefficient of variation