Asymmetric synthesis of a fluoxetine precursor with an artificial fusion protein of a ketoreductase and a formate dehydrogenase

•A fusion protein consisting of a formate dehydrogenase and a ketoreductase was created.•A proteolytic decay during purification of the fusion protein was observed.•After elucidation of the cleavage site a proteolytically stable fusion protein was obtained.•The fusion protein performed equal or even...

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Veröffentlicht in:	Process biochemistry (1991) 2014-09, Vol.49 (9), p.1527-1532
Hauptverfasser:	Sührer, Ilka, Haslbeck, Martin, Castiglione, Kathrin
Format:	Artikel
Sprache:	eng
Schlagworte:	Asymmetric reduction Asymmetry Biochemistry Chiral alcohol Cleavage Formate dehydrogenase Fusion protein Ketoreductase Mycobacterium vaccae Precursors Proteins Proteolytic degradation Purification Spreads Synechococcus
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