Bovine serum albumin-confined silver nanoclusters as fluorometric probe for detection of biothiols
ABSTRACT Fluorescent bovine serum albumin‐confined silver nanoclusters (BSA–AgNCs) were demonstrated to be a novel and environmentally friendly probe for the rapid detection of biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The sensing was ascribed to the strong affinity...
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| Veröffentlicht in: | Luminescence (Chichester, England) England), 2014-11, Vol.29 (7), p.722-727 |
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| creator | Chen, Zhen Lu, Dongtao Cai, Zongwei Dong, Chuan Shuang, Shaomin |
| description | ABSTRACT
Fluorescent bovine serum albumin‐confined silver nanoclusters (BSA–AgNCs) were demonstrated to be a novel and environmentally friendly probe for the rapid detection of biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The sensing was ascribed to the strong affinity between the mercapto group of the biothiols and the silver nanoclusters. The fluorescence intensity of BSA–AgNCs was quenched efficiently on increasing the concentration of biothiol, corresponding with a red‐shift in emission wavelength. However, the fluorescence of the silver nanoclusters was almost unchanged in the presence of other α‐amino acids at 10‐fold higher concentrations. By virtue of this specific response, a new, simple and rapid fluorescent method for detecting biothiols has been developed. The linear ranges for Cys, Hcy and GSH were 2.0 × 10‐6 to 9.0 × 10‐5 M (R2 = 0.994), 2.0 × 10‐6 to 1.2 × 10‐4 M (R2 = 0.996) and 1.0 × 10‐5 to 8.0 × 10‐5 M (R2 = 0.980), respectively. The detection limits were 8.1 × 10‐7 M for Cys, 1.0 × 10‐6 M for Hcy and 1.1 × 10‐6 M for GSH. Our proposed method was successfully applied to the determination of thiols in human plasma and the recovery was 94.83–105.24%. It is potentially applicable to protein‐stabilized silver nanoclusters in a chemical or biochemical sensing system. Copyright © 2014 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bio.2613 |
| format | Article |
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Fluorescent bovine serum albumin‐confined silver nanoclusters (BSA–AgNCs) were demonstrated to be a novel and environmentally friendly probe for the rapid detection of biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The sensing was ascribed to the strong affinity between the mercapto group of the biothiols and the silver nanoclusters. The fluorescence intensity of BSA–AgNCs was quenched efficiently on increasing the concentration of biothiol, corresponding with a red‐shift in emission wavelength. However, the fluorescence of the silver nanoclusters was almost unchanged in the presence of other α‐amino acids at 10‐fold higher concentrations. By virtue of this specific response, a new, simple and rapid fluorescent method for detecting biothiols has been developed. The linear ranges for Cys, Hcy and GSH were 2.0 × 10‐6 to 9.0 × 10‐5 M (R2 = 0.994), 2.0 × 10‐6 to 1.2 × 10‐4 M (R2 = 0.996) and 1.0 × 10‐5 to 8.0 × 10‐5 M (R2 = 0.980), respectively. The detection limits were 8.1 × 10‐7 M for Cys, 1.0 × 10‐6 M for Hcy and 1.1 × 10‐6 M for GSH. Our proposed method was successfully applied to the determination of thiols in human plasma and the recovery was 94.83–105.24%. It is potentially applicable to protein‐stabilized silver nanoclusters in a chemical or biochemical sensing system. Copyright © 2014 John Wiley & Sons, Ltd.</description><identifier>ISSN: 1522-7235</identifier><identifier>EISSN: 1522-7243</identifier><identifier>DOI: 10.1002/bio.2613</identifier><identifier>PMID: 24403131</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animals ; Biochemistry ; biothiols ; bovine serum albumin ; Cattle ; Detection ; Fluorescence ; Fluorescent Dyes - analysis ; Fluorescent Dyes - chemistry ; Fluorometers ; Humans ; Luminescence ; Metal Nanoparticles - chemistry ; Nanostructure ; Particle Size ; Serum Albumin, Bovine - chemistry ; Serums ; Silver ; Silver - chemistry ; silver nanoclusters ; Sulfhydryl Compounds - analysis ; Surface Properties</subject><ispartof>Luminescence (Chichester, England), 2014-11, Vol.29 (7), p.722-727</ispartof><rights>Copyright © 2014 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5893-bce5bb49f0c2702002fe9d32d962e5656559ef064a5f960e2f2ec1c7457fff6a3</citedby><cites>FETCH-LOGICAL-c5893-bce5bb49f0c2702002fe9d32d962e5656559ef064a5f960e2f2ec1c7457fff6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbio.2613$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbio.2613$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24403131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Zhen</creatorcontrib><creatorcontrib>Lu, Dongtao</creatorcontrib><creatorcontrib>Cai, Zongwei</creatorcontrib><creatorcontrib>Dong, Chuan</creatorcontrib><creatorcontrib>Shuang, Shaomin</creatorcontrib><title>Bovine serum albumin-confined silver nanoclusters as fluorometric probe for detection of biothiols</title><title>Luminescence (Chichester, England)</title><addtitle>Luminescence</addtitle><description>ABSTRACT
Fluorescent bovine serum albumin‐confined silver nanoclusters (BSA–AgNCs) were demonstrated to be a novel and environmentally friendly probe for the rapid detection of biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The sensing was ascribed to the strong affinity between the mercapto group of the biothiols and the silver nanoclusters. The fluorescence intensity of BSA–AgNCs was quenched efficiently on increasing the concentration of biothiol, corresponding with a red‐shift in emission wavelength. However, the fluorescence of the silver nanoclusters was almost unchanged in the presence of other α‐amino acids at 10‐fold higher concentrations. By virtue of this specific response, a new, simple and rapid fluorescent method for detecting biothiols has been developed. The linear ranges for Cys, Hcy and GSH were 2.0 × 10‐6 to 9.0 × 10‐5 M (R2 = 0.994), 2.0 × 10‐6 to 1.2 × 10‐4 M (R2 = 0.996) and 1.0 × 10‐5 to 8.0 × 10‐5 M (R2 = 0.980), respectively. The detection limits were 8.1 × 10‐7 M for Cys, 1.0 × 10‐6 M for Hcy and 1.1 × 10‐6 M for GSH. Our proposed method was successfully applied to the determination of thiols in human plasma and the recovery was 94.83–105.24%. It is potentially applicable to protein‐stabilized silver nanoclusters in a chemical or biochemical sensing system. Copyright © 2014 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>biothiols</subject><subject>bovine serum albumin</subject><subject>Cattle</subject><subject>Detection</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - analysis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorometers</subject><subject>Humans</subject><subject>Luminescence</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanostructure</subject><subject>Particle Size</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serums</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>silver nanoclusters</subject><subject>Sulfhydryl Compounds - analysis</subject><subject>Surface Properties</subject><issn>1522-7235</issn><issn>1522-7243</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U9rFDEYBvAgFlur4CeQgBcv0-bPJJkc3aWuldIiKD2GTOYNpmYmNZmp9tubpesiQqHkkBB-POTNg9AbSk4oIey0D-mEScqfoSMqGGsUa_nz_ZmLQ_SylBtCiJRSv0CHrG0Jp5weoX6V7sIEuEBeRmxjv4xhalyafL0dcAnxDjKe7JRcXMoMuWBbsI9LymmEOQeHb3PqAfuU8QAzuDmkCSeP65vm7yHF8godeBsLvN7tx-jbx7Ov60_NxdXmfP3honGi07zpHYi-b7UnjinC6lge9MDZoCUDIesSGjyRrRVeSwLMM3DUqVYo7720_Bi9f8itD_q5QJnNGIqDGO0EaSmGSqW07qigT6BM87YVunsCJZpTogmr9N1_9CYteaozbxXRXSfVP4Eup1IyeHObw2jzvaHEbNs09efMts1K3-4Cl36EYQ__1ldB8wB-hQj3jwaZ1fnVLnDnQ63y997b_MNIxZUw15cbo7-srj9vurVZ8z9D5bck</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Chen, Zhen</creator><creator>Lu, Dongtao</creator><creator>Cai, Zongwei</creator><creator>Dong, Chuan</creator><creator>Shuang, Shaomin</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H95</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L.G</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201411</creationdate><title>Bovine serum albumin-confined silver nanoclusters as fluorometric probe for detection of biothiols</title><author>Chen, Zhen ; Lu, Dongtao ; Cai, Zongwei ; Dong, Chuan ; Shuang, Shaomin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5893-bce5bb49f0c2702002fe9d32d962e5656559ef064a5f960e2f2ec1c7457fff6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>biothiols</topic><topic>bovine serum albumin</topic><topic>Cattle</topic><topic>Detection</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - analysis</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorometers</topic><topic>Humans</topic><topic>Luminescence</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanostructure</topic><topic>Particle Size</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serums</topic><topic>Silver</topic><topic>Silver - chemistry</topic><topic>silver nanoclusters</topic><topic>Sulfhydryl Compounds - analysis</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Zhen</creatorcontrib><creatorcontrib>Lu, Dongtao</creatorcontrib><creatorcontrib>Cai, Zongwei</creatorcontrib><creatorcontrib>Dong, Chuan</creatorcontrib><creatorcontrib>Shuang, Shaomin</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Luminescence (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Zhen</au><au>Lu, Dongtao</au><au>Cai, Zongwei</au><au>Dong, Chuan</au><au>Shuang, Shaomin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine serum albumin-confined silver nanoclusters as fluorometric probe for detection of biothiols</atitle><jtitle>Luminescence (Chichester, England)</jtitle><addtitle>Luminescence</addtitle><date>2014-11</date><risdate>2014</risdate><volume>29</volume><issue>7</issue><spage>722</spage><epage>727</epage><pages>722-727</pages><issn>1522-7235</issn><eissn>1522-7243</eissn><abstract>ABSTRACT
Fluorescent bovine serum albumin‐confined silver nanoclusters (BSA–AgNCs) were demonstrated to be a novel and environmentally friendly probe for the rapid detection of biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The sensing was ascribed to the strong affinity between the mercapto group of the biothiols and the silver nanoclusters. The fluorescence intensity of BSA–AgNCs was quenched efficiently on increasing the concentration of biothiol, corresponding with a red‐shift in emission wavelength. However, the fluorescence of the silver nanoclusters was almost unchanged in the presence of other α‐amino acids at 10‐fold higher concentrations. By virtue of this specific response, a new, simple and rapid fluorescent method for detecting biothiols has been developed. The linear ranges for Cys, Hcy and GSH were 2.0 × 10‐6 to 9.0 × 10‐5 M (R2 = 0.994), 2.0 × 10‐6 to 1.2 × 10‐4 M (R2 = 0.996) and 1.0 × 10‐5 to 8.0 × 10‐5 M (R2 = 0.980), respectively. The detection limits were 8.1 × 10‐7 M for Cys, 1.0 × 10‐6 M for Hcy and 1.1 × 10‐6 M for GSH. Our proposed method was successfully applied to the determination of thiols in human plasma and the recovery was 94.83–105.24%. It is potentially applicable to protein‐stabilized silver nanoclusters in a chemical or biochemical sensing system. Copyright © 2014 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24403131</pmid><doi>10.1002/bio.2613</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Biochemistry biothiols bovine serum albumin Cattle Detection Fluorescence Fluorescent Dyes - analysis Fluorescent Dyes - chemistry Fluorometers Humans Luminescence Metal Nanoparticles - chemistry Nanostructure Particle Size Serum Albumin, Bovine - chemistry Serums Silver Silver - chemistry silver nanoclusters Sulfhydryl Compounds - analysis Surface Properties |
| title | Bovine serum albumin-confined silver nanoclusters as fluorometric probe for detection of biothiols |
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