Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential Oil
Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct...
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description | Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. |
doi_str_mv | 10.4028/www.scientific.net/KEM.594-595.370 |
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It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. 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It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria.</description><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacillus</subject><subject>Bacillus subtilis</subject><subject>Bacteria</subject><subject>Biological</subject><subject>Cymbopogon flexuosus</subject><subject>Electrophoresis</subject><subject>Low concentrations</subject><subject>Proteins</subject><issn>1013-9826</issn><issn>1662-9795</issn><issn>1662-9795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqVkU1v1DAQhiMEEqXwH3xESEk9TuzYxzYsH6KoHMrZSpwxdeWNl4yjZf89rhaJKxxGM4dnXo3mqap3wJuOC311PB4bcgGXHHxwzYL56svuayNNV0sjm7bnz6oLUErUpjfyeZk5tLXRQr2sXhE9ct6CBnlR4bc1ZQwLK33eHM5sOrGb0YUYN2K0TTnEQOz6fhgEtK1gBc0PWHAkXByy5Nlw2k_pkH6khfmIv7ZEZXVH9HTdGNldiK-rF36MhG_-9Mvq-4fd_fCpvr37-Hm4vq1dxyHXigvZA3qtu66dnSjnKMc7PfejV8aANsaBFjBPWunWgemFFGKchJdg5tm3l9Xbc-5hTT83pGz3gRzGOC6YNrKg-t7oDoT-B1RwbnoFqqA3Z9StiWhFbw9r2I_ryQK3Tzps0WH_6rBFhy06bNFRStqio4S8P4fkdVwoo3uwj2lbl_KO_4n5DQZJnOU</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Muhamad, Hairul Shahril</creator><creator>Mat Radzi, Salina</creator><creator>Ismail, Ismatul Nurul Asyikin</creator><creator>Rehan, Maryam Mohamed</creator><creator>Mohamad, Rosfarizan</creator><creator>Mohd Noor, Hanina</creator><creator>Kader, Abdul Jalil Abdul</creator><general>Trans Tech Publications Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20131201</creationdate><title>Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential Oil</title><author>Muhamad, Hairul Shahril ; Mat Radzi, Salina ; Ismail, Ismatul Nurul Asyikin ; Rehan, Maryam Mohamed ; Mohamad, Rosfarizan ; Mohd Noor, Hanina ; Kader, Abdul Jalil Abdul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-602571ef88443dc2ced6c048d7af6991899c1821db8683c1972522ab2f519ddf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Bacillus</topic><topic>Bacillus subtilis</topic><topic>Bacteria</topic><topic>Biological</topic><topic>Cymbopogon flexuosus</topic><topic>Electrophoresis</topic><topic>Low concentrations</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muhamad, Hairul Shahril</creatorcontrib><creatorcontrib>Mat Radzi, Salina</creatorcontrib><creatorcontrib>Ismail, Ismatul Nurul Asyikin</creatorcontrib><creatorcontrib>Rehan, Maryam Mohamed</creatorcontrib><creatorcontrib>Mohamad, Rosfarizan</creatorcontrib><creatorcontrib>Mohd Noor, Hanina</creatorcontrib><creatorcontrib>Kader, Abdul Jalil Abdul</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Key engineering materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muhamad, Hairul Shahril</au><au>Mat Radzi, Salina</au><au>Ismail, Ismatul Nurul Asyikin</au><au>Rehan, Maryam Mohamed</au><au>Mohamad, Rosfarizan</au><au>Mohd Noor, Hanina</au><au>Kader, Abdul Jalil Abdul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential Oil</atitle><jtitle>Key engineering materials</jtitle><date>2013-12-01</date><risdate>2013</risdate><volume>594-595</volume><spage>370</spage><epage>377</epage><pages>370-377</pages><issn>1013-9826</issn><issn>1662-9795</issn><eissn>1662-9795</eissn><abstract>Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria.</abstract><pub>Trans Tech Publications Ltd</pub><doi>10.4028/www.scientific.net/KEM.594-595.370</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antibiotics Antiinfectives and antibacterials Bacillus Bacillus subtilis Bacteria Biological Cymbopogon flexuosus Electrophoresis Low concentrations Proteins |
title | Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential Oil |
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