A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes
A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horse...
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| Veröffentlicht in: | Biosensors & bioelectronics 2015-05, Vol.67, p.129-133 |
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| container_title | Biosensors & bioelectronics |
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| creator | Azmi, Nur Ellina Ramli, Noor Izaanin Abdullah, Jaafar Abdul Hamid, Mohammad Azmi Sidek, Hamidah Abd Rahman, Samsulida Ariffin, Nurhayati Yusof, Nor Azah |
| description | A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM. |
| doi_str_mv | 10.1016/j.bios.2014.07.056 |
| format | Article |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bioelectricity Biosensing Techniques Biosensors Biotechnology Fluorescence Fluorescence Resonance Energy Transfer Horseradish Peroxidase - chemistry Humans Hydrogen peroxide Hydrogen Peroxide - chemistry Hydrogen Peroxide - isolation & purification Peroxidase Quantum Dots Urate Oxidase - chemistry Uric acid Uric Acid - chemistry Uric Acid - isolation & purification Uric Acid - urine Urine |
| title | A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
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