Metabolomic analysis of the polyphenols in germinating mung beans (Vigna radiata) seeds and sprouts
BACKGROUND: The mung bean (Vigna radiata) is a key food crop in much of Asia and contains plentiful biological activities to prevent human disease. Mung bean sprouts have more plentiful metabolites and activities after germination. RESULTS: The metabolite profile of polyphenols in the germination pr...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2014-06, Vol.94 (8), p.1639-1647 |
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| creator | Tang, Dongyan Dong, Yinmao Guo, Na Li, Li Ren, Hankun |
| description | BACKGROUND: The mung bean (Vigna radiata) is a key food crop in much of Asia and contains plentiful biological activities to prevent human disease. Mung bean sprouts have more plentiful metabolites and activities after germination. RESULTS: The metabolite profile of polyphenols in the germination process was described using the methods of ultra‐high‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry and partial least squares discriminant analysis. Sprouts from different periods were clearly discriminated from each other. Eight flavonoids – vitexin, isovitexin, rutin, kaempferol 3‐O‐rutinoside, isoquercitrin, genistein, daidzein and isorhamnetin – and two phenolic acids – shikimic acid and caffeic acid – were thought to be chemical markers of the sprouts. The method of high‐performance liquid chromatography with diode array detection was established to quantitatively analyze the eight chemical markers of flavonoids, and provides good linearity, repeatability, intra‐ and inter‐day precision, accuracy and recovery. The main metabolic and transformation pathways of the polyphenols in the germination process were discussed. CONCLUSION: The proposed method is sensitive, rapid and robust. Understanding the complete profile of polyphenol metabolites in the germination process may be useful for better utilizing mung beans sprouts as the raw materials of functional food, health products and cosmetics. © 2013 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.6471 |
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Mung bean sprouts have more plentiful metabolites and activities after germination. RESULTS: The metabolite profile of polyphenols in the germination process was described using the methods of ultra‐high‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry and partial least squares discriminant analysis. Sprouts from different periods were clearly discriminated from each other. Eight flavonoids – vitexin, isovitexin, rutin, kaempferol 3‐O‐rutinoside, isoquercitrin, genistein, daidzein and isorhamnetin – and two phenolic acids – shikimic acid and caffeic acid – were thought to be chemical markers of the sprouts. The method of high‐performance liquid chromatography with diode array detection was established to quantitatively analyze the eight chemical markers of flavonoids, and provides good linearity, repeatability, intra‐ and inter‐day precision, accuracy and recovery. The main metabolic and transformation pathways of the polyphenols in the germination process were discussed. CONCLUSION: The proposed method is sensitive, rapid and robust. Understanding the complete profile of polyphenol metabolites in the germination process may be useful for better utilizing mung beans sprouts as the raw materials of functional food, health products and cosmetics. © 2013 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.6471</identifier><identifier>PMID: 24203396</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>bean sprouts ; Beans ; caffeic acid ; Caffeic Acids - analysis ; Chromatography ; Chromatography, High Pressure Liquid ; cosmetics ; daidzein ; discriminant analysis ; discrimination ; Fabaceae - physiology ; Flavonoids ; Flavonoids - analysis ; Foods ; functional foods ; genistein ; Germination ; isoquercitrin ; kaempferol ; least squares ; Liquid chromatography ; Markers ; Mass Spectrometry ; Metabolism ; Metabolites ; Metabolomics ; Multivariate Analysis ; mung beans ; mung beans sprouts ; PLS-DA ; polyphenol profiling ; Polyphenols ; Polyphenols - analysis ; raw materials ; Reproducibility of Results ; rutin ; Seedlings - chemistry ; Seeds ; Seeds - chemistry ; Seeds - growth & development ; Sensitivity and Specificity ; shikimic acid ; Shikimic Acid - analysis ; UPLC-Q-TOF-MS ; Vigna radiata</subject><ispartof>Journal of the science of food and agriculture, 2014-06, Vol.94 (8), p.1639-1647</ispartof><rights>2013 Society of Chemical Industry</rights><rights>2013 Society of Chemical Industry.</rights><rights>Copyright John Wiley and Sons, Limited Jun 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4811-174e911352be2003772599ba14b779e57897d107a10c9b46135a385bccf80c053</citedby><cites>FETCH-LOGICAL-c4811-174e911352be2003772599ba14b779e57897d107a10c9b46135a385bccf80c053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.6471$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.6471$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24203396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Dongyan</creatorcontrib><creatorcontrib>Dong, Yinmao</creatorcontrib><creatorcontrib>Guo, Na</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Ren, Hankun</creatorcontrib><title>Metabolomic analysis of the polyphenols in germinating mung beans (Vigna radiata) seeds and sprouts</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>BACKGROUND: The mung bean (Vigna radiata) is a key food crop in much of Asia and contains plentiful biological activities to prevent human disease. Mung bean sprouts have more plentiful metabolites and activities after germination. RESULTS: The metabolite profile of polyphenols in the germination process was described using the methods of ultra‐high‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry and partial least squares discriminant analysis. Sprouts from different periods were clearly discriminated from each other. Eight flavonoids – vitexin, isovitexin, rutin, kaempferol 3‐O‐rutinoside, isoquercitrin, genistein, daidzein and isorhamnetin – and two phenolic acids – shikimic acid and caffeic acid – were thought to be chemical markers of the sprouts. The method of high‐performance liquid chromatography with diode array detection was established to quantitatively analyze the eight chemical markers of flavonoids, and provides good linearity, repeatability, intra‐ and inter‐day precision, accuracy and recovery. The main metabolic and transformation pathways of the polyphenols in the germination process were discussed. CONCLUSION: The proposed method is sensitive, rapid and robust. Understanding the complete profile of polyphenol metabolites in the germination process may be useful for better utilizing mung beans sprouts as the raw materials of functional food, health products and cosmetics. © 2013 Society of Chemical Industry</description><subject>bean sprouts</subject><subject>Beans</subject><subject>caffeic acid</subject><subject>Caffeic Acids - analysis</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>cosmetics</subject><subject>daidzein</subject><subject>discriminant analysis</subject><subject>discrimination</subject><subject>Fabaceae - physiology</subject><subject>Flavonoids</subject><subject>Flavonoids - analysis</subject><subject>Foods</subject><subject>functional foods</subject><subject>genistein</subject><subject>Germination</subject><subject>isoquercitrin</subject><subject>kaempferol</subject><subject>least squares</subject><subject>Liquid chromatography</subject><subject>Markers</subject><subject>Mass Spectrometry</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Multivariate Analysis</subject><subject>mung beans</subject><subject>mung beans sprouts</subject><subject>PLS-DA</subject><subject>polyphenol profiling</subject><subject>Polyphenols</subject><subject>Polyphenols - analysis</subject><subject>raw materials</subject><subject>Reproducibility of Results</subject><subject>rutin</subject><subject>Seedlings - chemistry</subject><subject>Seeds</subject><subject>Seeds - chemistry</subject><subject>Seeds - growth & development</subject><subject>Sensitivity and Specificity</subject><subject>shikimic acid</subject><subject>Shikimic Acid - analysis</subject><subject>UPLC-Q-TOF-MS</subject><subject>Vigna radiata</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0stu1DAUBmALgehQWPACYKmbdpH2HMeXeFkVZgCVglQ6LC0ncaYechniRDBvj6MMXSAh2NgLf-e35N-EvEQ4RwB2sQ2VPZdc4SOyQNAqAUB4TBbxjCUCOTsiz0LYAoDWUj4lR4wzSFMtF6T46Aabd3XX-ILa1tb74APtKjrcO7rr6v3u3rVdHahv6cb1jW_t4NsNbca45M62gZ6u_aa1tLelt4M9o8G5MsSskoZd341DeE6eVLYO7sVhPyZ3y7dfrt4l159W768ur5OCZ4gJKu40YipY7hhAqhQTWucWea6UdkJlWpUIyiIUOucySptmIi-KKoMCRHpMTufceO330YXBND4Urq5t67oxGJQxJ0OeyX9TIRA4SK7_gzKhZAaKRXryB912Yx8fdVIpoAYps6jOZlX0XQi9q8yu943t9wbBTH2aqU8z9Rntq0PimDeufJC_C4zgYgY_fO32f08yH26Xl4fIZJ7wYXA_HyZs_81IlSphvt6sDK4-vxHr5drcRP969pXtjN30Ppi7WwbIp0_GFKr0F7q2v3Y</recordid><startdate>201406</startdate><enddate>201406</enddate><creator>Tang, Dongyan</creator><creator>Dong, Yinmao</creator><creator>Guo, Na</creator><creator>Li, Li</creator><creator>Ren, Hankun</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201406</creationdate><title>Metabolomic analysis of the polyphenols in germinating mung beans (Vigna radiata) seeds and sprouts</title><author>Tang, Dongyan ; Dong, Yinmao ; Guo, Na ; Li, Li ; Ren, Hankun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4811-174e911352be2003772599ba14b779e57897d107a10c9b46135a385bccf80c053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>bean sprouts</topic><topic>Beans</topic><topic>caffeic acid</topic><topic>Caffeic Acids - analysis</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>cosmetics</topic><topic>daidzein</topic><topic>discriminant analysis</topic><topic>discrimination</topic><topic>Fabaceae - physiology</topic><topic>Flavonoids</topic><topic>Flavonoids - analysis</topic><topic>Foods</topic><topic>functional foods</topic><topic>genistein</topic><topic>Germination</topic><topic>isoquercitrin</topic><topic>kaempferol</topic><topic>least squares</topic><topic>Liquid chromatography</topic><topic>Markers</topic><topic>Mass Spectrometry</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Multivariate Analysis</topic><topic>mung beans</topic><topic>mung beans sprouts</topic><topic>PLS-DA</topic><topic>polyphenol profiling</topic><topic>Polyphenols</topic><topic>Polyphenols - analysis</topic><topic>raw materials</topic><topic>Reproducibility of Results</topic><topic>rutin</topic><topic>Seedlings - chemistry</topic><topic>Seeds</topic><topic>Seeds - chemistry</topic><topic>Seeds - growth & development</topic><topic>Sensitivity and Specificity</topic><topic>shikimic acid</topic><topic>Shikimic Acid - analysis</topic><topic>UPLC-Q-TOF-MS</topic><topic>Vigna radiata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Dongyan</creatorcontrib><creatorcontrib>Dong, Yinmao</creatorcontrib><creatorcontrib>Guo, Na</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Ren, Hankun</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Dongyan</au><au>Dong, Yinmao</au><au>Guo, Na</au><au>Li, Li</au><au>Ren, Hankun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolomic analysis of the polyphenols in germinating mung beans (Vigna radiata) seeds and sprouts</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2014-06</date><risdate>2014</risdate><volume>94</volume><issue>8</issue><spage>1639</spage><epage>1647</epage><pages>1639-1647</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>BACKGROUND: The mung bean (Vigna radiata) is a key food crop in much of Asia and contains plentiful biological activities to prevent human disease. Mung bean sprouts have more plentiful metabolites and activities after germination. RESULTS: The metabolite profile of polyphenols in the germination process was described using the methods of ultra‐high‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry and partial least squares discriminant analysis. Sprouts from different periods were clearly discriminated from each other. Eight flavonoids – vitexin, isovitexin, rutin, kaempferol 3‐O‐rutinoside, isoquercitrin, genistein, daidzein and isorhamnetin – and two phenolic acids – shikimic acid and caffeic acid – were thought to be chemical markers of the sprouts. The method of high‐performance liquid chromatography with diode array detection was established to quantitatively analyze the eight chemical markers of flavonoids, and provides good linearity, repeatability, intra‐ and inter‐day precision, accuracy and recovery. The main metabolic and transformation pathways of the polyphenols in the germination process were discussed. CONCLUSION: The proposed method is sensitive, rapid and robust. Understanding the complete profile of polyphenol metabolites in the germination process may be useful for better utilizing mung beans sprouts as the raw materials of functional food, health products and cosmetics. © 2013 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>24203396</pmid><doi>10.1002/jsfa.6471</doi><tpages>9</tpages></addata></record> |
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| subjects | bean sprouts Beans caffeic acid Caffeic Acids - analysis Chromatography Chromatography, High Pressure Liquid cosmetics daidzein discriminant analysis discrimination Fabaceae - physiology Flavonoids Flavonoids - analysis Foods functional foods genistein Germination isoquercitrin kaempferol least squares Liquid chromatography Markers Mass Spectrometry Metabolism Metabolites Metabolomics Multivariate Analysis mung beans mung beans sprouts PLS-DA polyphenol profiling Polyphenols Polyphenols - analysis raw materials Reproducibility of Results rutin Seedlings - chemistry Seeds Seeds - chemistry Seeds - growth & development Sensitivity and Specificity shikimic acid Shikimic Acid - analysis UPLC-Q-TOF-MS Vigna radiata |
| title | Metabolomic analysis of the polyphenols in germinating mung beans (Vigna radiata) seeds and sprouts |
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