A non-aggregation colorimetric assay for thrombin based on catalytic properties of silver nanoparticles

•An AgNP-based non-aggregation colorimetric aptasensor was first developed.•The colorimetric principle was based on AgNP-catalyzed reductive degradation of RhB.•This assay combined magnetic separation with nanocatalytic amplification.•The detection limit of thrombin was as low as 0.2nM with excellen...

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