Functional graphene-gold nano-composite fabricated electrochemical biosensor for direct and rapid detection of bisphenol A

[Display omitted] •A novel graphene was fabricated via gold nanoparticle to separate individual sheets.•Various techniques are adopted to characterize the prepared nanocomposites.•A tyrosinase biosensor based on graphene-gold nanocomposites was developed.•The proposed biosensor for BPA detection exhibited excellent performance. In this research, the graphene with excellent dispersity is prepared successfully by introducing gold nanoparticle to separate the individual sheets. Various techniques are adopted to characterize the prepared graphene and graphene-gold nanoparticle composite materials. This fabricated new composite material is used as the support material to construct a novel tyrosinase based biosensor for detection of bisphenol A (BPA). The electrochemical performances of the proposed new enzyme biosensor were investigated by differential pulse voltammetry (DPV) method. The proposed biosensor exhibited excellent performance for BPA determination with a wide linear range (2.5×10−3–3.0μM), a highly reproducible response (RSD of 2.7%), low interferences and long-term stability. And more importantly, the calculated detection limit of the proposed biosensor was as low as 1nM. Compared with other detection methods, this graphene-gold nanoparticle composite based tyrosinase biosensor is proved to be a promising and reliable tool for rapid detection of BPA for on-site analysis of emergency BPA related pollution affairs.