Occurrence and fate of potential pathogenic bacteria as revealed by pyrosequencing in a full-scale membrane bioreactor treating restaurant wastewater
One of the primary concerns on wastewater reuse is the presence of pathogenic bacteria. Considering that indicator bacteria might only offer limited information, we applied high-throughput pyrosequencing in this study to reveal bacterial pathogen diversity in a full-scale membrane bioreactor (MBR) t...
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| Veröffentlicht in: | RSC advances 2015-01, Vol.5 (31), p.24469-24478 |
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| creator | Ma, Jinxing Wang, Zhiwei Zang, Lili Huang, Jian Wu, Zhichao |
| description | One of the primary concerns on wastewater reuse is the presence of pathogenic bacteria. Considering that indicator bacteria might only offer limited information, we applied high-throughput pyrosequencing in this study to reveal bacterial pathogen diversity in a full-scale membrane bioreactor (MBR) treating restaurant wastewater. The results showed that fecal indicator bacteria could provide a rough estimation rather than an accurate characterization of the potential pathogenic bacteria in wastewaters, particularly from non-fecal sources. In general, MBR treatment showed good removal of potential pathogenic bacteria. The bacterial count of Arcobacterwas decreased by nearly seven orders of magnitude, from (8.35 plus or minus 0.87) 10 super(7) to 99% to the neighbor pathogenic species, including Arcobacter butzleriand Arcobacter cryaerophilus. This study, therefore, provides insights into the occurrence and fate of potential bacterial pathogens in restaurant wastewater treatment and reclamation using MBRs. |
| doi_str_mv | 10.1039/c4ra10220g |
| format | Article |
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Considering that indicator bacteria might only offer limited information, we applied high-throughput pyrosequencing in this study to reveal bacterial pathogen diversity in a full-scale membrane bioreactor (MBR) treating restaurant wastewater. The results showed that fecal indicator bacteria could provide a rough estimation rather than an accurate characterization of the potential pathogenic bacteria in wastewaters, particularly from non-fecal sources. In general, MBR treatment showed good removal of potential pathogenic bacteria. The bacterial count of Arcobacterwas decreased by nearly seven orders of magnitude, from (8.35 plus or minus 0.87) 10 super(7) to <10 counts per mL, and Aeromonas, Enterobacter, Enterococcus, and Pseudomonaswere not detected in the treated wastewater. The most dominant potential pathogens in activated sludge and treated wastewater were affiliated to the genera of Legionella, Clostridiumand Mycobacterium. Species-specific comparison showed that only a small portion (0.0-1.6%) of the corresponding sequences had identities of > 99% to the neighbor pathogenic species, including Arcobacter butzleriand Arcobacter cryaerophilus. This study, therefore, provides insights into the occurrence and fate of potential bacterial pathogens in restaurant wastewater treatment and reclamation using MBRs.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c4ra10220g</identifier><language>eng</language><subject>Aeromonas ; Arcobacter ; Arcobacter cryaerophilus ; Bacteria ; Bioreactors ; Counting ; Enterobacter ; Enterococcus ; Indicators ; Legionella ; Membranes ; Mycobacterium ; Pathogens ; Restaurants ; Wastewater treatment</subject><ispartof>RSC advances, 2015-01, Vol.5 (31), p.24469-24478</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c297t-c0f8ffb89c4f24588187bfe906aa1c2d478655de074b12e053857e7e9a61c3163</citedby><cites>FETCH-LOGICAL-c297t-c0f8ffb89c4f24588187bfe906aa1c2d478655de074b12e053857e7e9a61c3163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Ma, Jinxing</creatorcontrib><creatorcontrib>Wang, Zhiwei</creatorcontrib><creatorcontrib>Zang, Lili</creatorcontrib><creatorcontrib>Huang, Jian</creatorcontrib><creatorcontrib>Wu, Zhichao</creatorcontrib><title>Occurrence and fate of potential pathogenic bacteria as revealed by pyrosequencing in a full-scale membrane bioreactor treating restaurant wastewater</title><title>RSC advances</title><description>One of the primary concerns on wastewater reuse is the presence of pathogenic bacteria. Considering that indicator bacteria might only offer limited information, we applied high-throughput pyrosequencing in this study to reveal bacterial pathogen diversity in a full-scale membrane bioreactor (MBR) treating restaurant wastewater. The results showed that fecal indicator bacteria could provide a rough estimation rather than an accurate characterization of the potential pathogenic bacteria in wastewaters, particularly from non-fecal sources. In general, MBR treatment showed good removal of potential pathogenic bacteria. The bacterial count of Arcobacterwas decreased by nearly seven orders of magnitude, from (8.35 plus or minus 0.87) 10 super(7) to <10 counts per mL, and Aeromonas, Enterobacter, Enterococcus, and Pseudomonaswere not detected in the treated wastewater. The most dominant potential pathogens in activated sludge and treated wastewater were affiliated to the genera of Legionella, Clostridiumand Mycobacterium. Species-specific comparison showed that only a small portion (0.0-1.6%) of the corresponding sequences had identities of > 99% to the neighbor pathogenic species, including Arcobacter butzleriand Arcobacter cryaerophilus. This study, therefore, provides insights into the occurrence and fate of potential bacterial pathogens in restaurant wastewater treatment and reclamation using MBRs.</description><subject>Aeromonas</subject><subject>Arcobacter</subject><subject>Arcobacter cryaerophilus</subject><subject>Bacteria</subject><subject>Bioreactors</subject><subject>Counting</subject><subject>Enterobacter</subject><subject>Enterococcus</subject><subject>Indicators</subject><subject>Legionella</subject><subject>Membranes</subject><subject>Mycobacterium</subject><subject>Pathogens</subject><subject>Restaurants</subject><subject>Wastewater treatment</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkUFLw0AQhYMoWLQXf8EcRYjubja7ybEUrUKhIHoOk-1sjaRJ3N1Y-kP8v26tB4_OZR7Mx-MxL0muOLvlLCvvjHTImRBsc5JMBJMqFUyVp3_0eTL1_p3FUTkXik-Sr5Uxo3PUGQLs1mAxEPQWhj5QFxpsYcDw1m-oawzUaAK5BgE9OPokbGkN9R6Gves9fYzRpek20HSAYMe2Tb2JCGxpWzvsCOqmdxQ9egchinCAHfmAYzwH2KEPtIsB3GVyZrH1NP3dF8nrw_3L_DFdrhZP89kyNaLUITXMFtbWRWmkFTIvCl7o2lLJFCI3Yi11ofJ8TUzLmgtieVbkmjSVqLjJuMoukuuj7-D6GN-Hatt4Q20b0_ajr7jSupSFVOw_qMryTGc8ojdH1MS3eEe2GlyzRbevOKsOTVVz-Tz7aWqRfQM_WYjh</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Ma, Jinxing</creator><creator>Wang, Zhiwei</creator><creator>Zang, Lili</creator><creator>Huang, Jian</creator><creator>Wu, Zhichao</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>KR7</scope><scope>L7M</scope></search><sort><creationdate>20150101</creationdate><title>Occurrence and fate of potential pathogenic bacteria as revealed by pyrosequencing in a full-scale membrane bioreactor treating restaurant wastewater</title><author>Ma, Jinxing ; Wang, Zhiwei ; Zang, Lili ; Huang, Jian ; Wu, Zhichao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-c0f8ffb89c4f24588187bfe906aa1c2d478655de074b12e053857e7e9a61c3163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aeromonas</topic><topic>Arcobacter</topic><topic>Arcobacter cryaerophilus</topic><topic>Bacteria</topic><topic>Bioreactors</topic><topic>Counting</topic><topic>Enterobacter</topic><topic>Enterococcus</topic><topic>Indicators</topic><topic>Legionella</topic><topic>Membranes</topic><topic>Mycobacterium</topic><topic>Pathogens</topic><topic>Restaurants</topic><topic>Wastewater treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Jinxing</creatorcontrib><creatorcontrib>Wang, Zhiwei</creatorcontrib><creatorcontrib>Zang, Lili</creatorcontrib><creatorcontrib>Huang, Jian</creatorcontrib><creatorcontrib>Wu, Zhichao</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Jinxing</au><au>Wang, Zhiwei</au><au>Zang, Lili</au><au>Huang, Jian</au><au>Wu, Zhichao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and fate of potential pathogenic bacteria as revealed by pyrosequencing in a full-scale membrane bioreactor treating restaurant wastewater</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>31</issue><spage>24469</spage><epage>24478</epage><pages>24469-24478</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>One of the primary concerns on wastewater reuse is the presence of pathogenic bacteria. Considering that indicator bacteria might only offer limited information, we applied high-throughput pyrosequencing in this study to reveal bacterial pathogen diversity in a full-scale membrane bioreactor (MBR) treating restaurant wastewater. The results showed that fecal indicator bacteria could provide a rough estimation rather than an accurate characterization of the potential pathogenic bacteria in wastewaters, particularly from non-fecal sources. In general, MBR treatment showed good removal of potential pathogenic bacteria. The bacterial count of Arcobacterwas decreased by nearly seven orders of magnitude, from (8.35 plus or minus 0.87) 10 super(7) to <10 counts per mL, and Aeromonas, Enterobacter, Enterococcus, and Pseudomonaswere not detected in the treated wastewater. The most dominant potential pathogens in activated sludge and treated wastewater were affiliated to the genera of Legionella, Clostridiumand Mycobacterium. Species-specific comparison showed that only a small portion (0.0-1.6%) of the corresponding sequences had identities of > 99% to the neighbor pathogenic species, including Arcobacter butzleriand Arcobacter cryaerophilus. This study, therefore, provides insights into the occurrence and fate of potential bacterial pathogens in restaurant wastewater treatment and reclamation using MBRs.</abstract><doi>10.1039/c4ra10220g</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 2046-2069 |
| ispartof | RSC advances, 2015-01, Vol.5 (31), p.24469-24478 |
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| language | eng |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Aeromonas Arcobacter Arcobacter cryaerophilus Bacteria Bioreactors Counting Enterobacter Enterococcus Indicators Legionella Membranes Mycobacterium Pathogens Restaurants Wastewater treatment |
| title | Occurrence and fate of potential pathogenic bacteria as revealed by pyrosequencing in a full-scale membrane bioreactor treating restaurant wastewater |
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