Degradable Poly(amidoamine) Hydrogels as Scaffolds for In Vitro Culturing of Peripheral Nervous System Cells
This paper reports on the synthesis and physico‐chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N′‐methylenebis(acrylami...
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| Veröffentlicht in: | Macromolecular bioscience 2013-03, Vol.13 (3), p.332-347 |
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| creator | Mauro, Nicolò Manfredi, Amedea Ranucci, Elisabetta Procacci, Patrizia Laus, Michele Antonioli, Diego Mantovani, Cristina Magnaghi, Valerio Ferruti, Paolo |
| description | This paper reports on the synthesis and physico‐chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N′‐methylenebis(acrylamide) or 1,4‐bis(acryloyl)piperazine with 1,2‐diaminoethane as cross‐linking agent and exhibit a combination of relevant properties, such as mechanical strength, biocompatibility, biodegradability, ability to induce adhesion and proliferation of Schwann cells (SCs) preserving their viability. Moreover, the most promising hydrogels, that is those deriving from 1,4‐bis(acryloyl)piperazine, allow the in vitro growth of the sensitive neurons of the dorsal root ganglia, thus getting around a critical point in the design of conduits for nerve regeneration.
Poly(amidoamine) hydrogels based on piperazine and 1,4‐bis(acryloyl)piperazine are robust in the swollen state due to crystalline domains acting as self‐reinforcing agents and promote Schwann and dorsal root ganglia neuron proliferation. Their combination of properties is seldom found in synthetic scaffolds for peripheral nerve cell culturing. |
| doi_str_mv | 10.1002/mabi.201200354 |
| format | Article |
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Poly(amidoamine) hydrogels based on piperazine and 1,4‐bis(acryloyl)piperazine are robust in the swollen state due to crystalline domains acting as self‐reinforcing agents and promote Schwann and dorsal root ganglia neuron proliferation. Their combination of properties is seldom found in synthetic scaffolds for peripheral nerve cell culturing.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.201200354</identifier><identifier>PMID: 23239646</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Acrylamides - chemistry ; Animals ; Biocompatibility ; Biocompatible Materials - chemical synthesis ; Biocompatible Materials - pharmacology ; biodegradable ; Cell Adhesion - drug effects ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Ethylenediamines - chemistry ; Ganglia ; Ganglia, Spinal - cytology ; Ganglia, Spinal - drug effects ; Hydrogels ; Hydrogels - chemistry ; In vitro testing ; Nerve Regeneration ; neural cell culturing ; Neurons ; Neurons - cytology ; Neurons - drug effects ; Peripheral nerves ; Piperazines - chemistry ; poly(amidoamine) ; Polyamines - chemistry ; polymer applications ; Rats ; Rats, Sprague-Dawley ; Regeneration ; Roots ; Scaffolds ; Schwann Cells - cytology ; Schwann Cells - drug effects ; Tissue Engineering ; Tissue Scaffolds</subject><ispartof>Macromolecular bioscience, 2013-03, Vol.13 (3), p.332-347</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4164-dc0863fe1a02841ffb321a7fee6685a453b1bc61fe59ea317dfa0837883b71823</citedby><cites>FETCH-LOGICAL-c4164-dc0863fe1a02841ffb321a7fee6685a453b1bc61fe59ea317dfa0837883b71823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.201200354$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.201200354$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23239646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mauro, Nicolò</creatorcontrib><creatorcontrib>Manfredi, Amedea</creatorcontrib><creatorcontrib>Ranucci, Elisabetta</creatorcontrib><creatorcontrib>Procacci, Patrizia</creatorcontrib><creatorcontrib>Laus, Michele</creatorcontrib><creatorcontrib>Antonioli, Diego</creatorcontrib><creatorcontrib>Mantovani, Cristina</creatorcontrib><creatorcontrib>Magnaghi, Valerio</creatorcontrib><creatorcontrib>Ferruti, Paolo</creatorcontrib><title>Degradable Poly(amidoamine) Hydrogels as Scaffolds for In Vitro Culturing of Peripheral Nervous System Cells</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>This paper reports on the synthesis and physico‐chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N′‐methylenebis(acrylamide) or 1,4‐bis(acryloyl)piperazine with 1,2‐diaminoethane as cross‐linking agent and exhibit a combination of relevant properties, such as mechanical strength, biocompatibility, biodegradability, ability to induce adhesion and proliferation of Schwann cells (SCs) preserving their viability. Moreover, the most promising hydrogels, that is those deriving from 1,4‐bis(acryloyl)piperazine, allow the in vitro growth of the sensitive neurons of the dorsal root ganglia, thus getting around a critical point in the design of conduits for nerve regeneration.
Poly(amidoamine) hydrogels based on piperazine and 1,4‐bis(acryloyl)piperazine are robust in the swollen state due to crystalline domains acting as self‐reinforcing agents and promote Schwann and dorsal root ganglia neuron proliferation. Their combination of properties is seldom found in synthetic scaffolds for peripheral nerve cell culturing.</description><subject>Acrylamides - chemistry</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemical synthesis</subject><subject>Biocompatible Materials - pharmacology</subject><subject>biodegradable</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Ethylenediamines - chemistry</subject><subject>Ganglia</subject><subject>Ganglia, Spinal - cytology</subject><subject>Ganglia, Spinal - drug effects</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>In vitro testing</subject><subject>Nerve Regeneration</subject><subject>neural cell culturing</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Peripheral nerves</subject><subject>Piperazines - chemistry</subject><subject>poly(amidoamine)</subject><subject>Polyamines - chemistry</subject><subject>polymer applications</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration</subject><subject>Roots</subject><subject>Scaffolds</subject><subject>Schwann Cells - cytology</subject><subject>Schwann Cells - drug effects</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoh9w5Yh8LIcsnvgzx24K7UplWQkoR8tJxkvAWW_tDSX_nqy2rLj14vHheR-N5s2yN0BnQGnxvrd1NysoFJQywZ9lpyBB5gJK8fz41-okO0vpJ6WgdFm8zE4KVrBScnma-StcR9va2iNZBT9e2L5rw_Rs8B25GdsY1ugTsYl8aaxzwbeJuBDJYkPuul0MpBr8bojdZk2CIyuM3fYHRuvJEuPvMEyxMe2wJxV6n15lL5z1CV8_zvPs28cPX6ub_Pbz9aK6vM0bDpLnbUO1ZA7B0kJzcK5mBVjlEKXUwnLBaqgbCQ5FiZaBap2lmimtWa1AF-w8uzh4tzHcD5h2pu9SM21gNzjtZEAqVTKuBX8aZVCC5prurbMD2sSQUkRntrHrbRwNULMvw-zLMMcypsDbR_dQ99ge8X_Xn4DyADx0HscndObT5Xzxvzw_ZLvpvn-OWRt_GamYEub78trcXYlVNV_OzZz9BS4ypPI</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Mauro, Nicolò</creator><creator>Manfredi, Amedea</creator><creator>Ranucci, Elisabetta</creator><creator>Procacci, Patrizia</creator><creator>Laus, Michele</creator><creator>Antonioli, Diego</creator><creator>Mantovani, Cristina</creator><creator>Magnaghi, Valerio</creator><creator>Ferruti, Paolo</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>201303</creationdate><title>Degradable Poly(amidoamine) Hydrogels as Scaffolds for In Vitro Culturing of Peripheral Nervous System Cells</title><author>Mauro, Nicolò ; Manfredi, Amedea ; Ranucci, Elisabetta ; Procacci, Patrizia ; Laus, Michele ; Antonioli, Diego ; Mantovani, Cristina ; Magnaghi, Valerio ; Ferruti, Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4164-dc0863fe1a02841ffb321a7fee6685a453b1bc61fe59ea317dfa0837883b71823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acrylamides - chemistry</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemical synthesis</topic><topic>Biocompatible Materials - pharmacology</topic><topic>biodegradable</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Ethylenediamines - chemistry</topic><topic>Ganglia</topic><topic>Ganglia, Spinal - cytology</topic><topic>Ganglia, Spinal - drug effects</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>In vitro testing</topic><topic>Nerve Regeneration</topic><topic>neural cell culturing</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Peripheral nerves</topic><topic>Piperazines - chemistry</topic><topic>poly(amidoamine)</topic><topic>Polyamines - chemistry</topic><topic>polymer applications</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration</topic><topic>Roots</topic><topic>Scaffolds</topic><topic>Schwann Cells - cytology</topic><topic>Schwann Cells - drug effects</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mauro, Nicolò</creatorcontrib><creatorcontrib>Manfredi, Amedea</creatorcontrib><creatorcontrib>Ranucci, Elisabetta</creatorcontrib><creatorcontrib>Procacci, Patrizia</creatorcontrib><creatorcontrib>Laus, Michele</creatorcontrib><creatorcontrib>Antonioli, Diego</creatorcontrib><creatorcontrib>Mantovani, Cristina</creatorcontrib><creatorcontrib>Magnaghi, Valerio</creatorcontrib><creatorcontrib>Ferruti, Paolo</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mauro, Nicolò</au><au>Manfredi, Amedea</au><au>Ranucci, Elisabetta</au><au>Procacci, Patrizia</au><au>Laus, Michele</au><au>Antonioli, Diego</au><au>Mantovani, Cristina</au><au>Magnaghi, Valerio</au><au>Ferruti, Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradable Poly(amidoamine) Hydrogels as Scaffolds for In Vitro Culturing of Peripheral Nervous System Cells</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2013-03</date><risdate>2013</risdate><volume>13</volume><issue>3</issue><spage>332</spage><epage>347</epage><pages>332-347</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>This paper reports on the synthesis and physico‐chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N′‐methylenebis(acrylamide) or 1,4‐bis(acryloyl)piperazine with 1,2‐diaminoethane as cross‐linking agent and exhibit a combination of relevant properties, such as mechanical strength, biocompatibility, biodegradability, ability to induce adhesion and proliferation of Schwann cells (SCs) preserving their viability. Moreover, the most promising hydrogels, that is those deriving from 1,4‐bis(acryloyl)piperazine, allow the in vitro growth of the sensitive neurons of the dorsal root ganglia, thus getting around a critical point in the design of conduits for nerve regeneration.
Poly(amidoamine) hydrogels based on piperazine and 1,4‐bis(acryloyl)piperazine are robust in the swollen state due to crystalline domains acting as self‐reinforcing agents and promote Schwann and dorsal root ganglia neuron proliferation. Their combination of properties is seldom found in synthetic scaffolds for peripheral nerve cell culturing.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>23239646</pmid><doi>10.1002/mabi.201200354</doi><tpages>16</tpages></addata></record> |
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| subjects | Acrylamides - chemistry Animals Biocompatibility Biocompatible Materials - chemical synthesis Biocompatible Materials - pharmacology biodegradable Cell Adhesion - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Ethylenediamines - chemistry Ganglia Ganglia, Spinal - cytology Ganglia, Spinal - drug effects Hydrogels Hydrogels - chemistry In vitro testing Nerve Regeneration neural cell culturing Neurons Neurons - cytology Neurons - drug effects Peripheral nerves Piperazines - chemistry poly(amidoamine) Polyamines - chemistry polymer applications Rats Rats, Sprague-Dawley Regeneration Roots Scaffolds Schwann Cells - cytology Schwann Cells - drug effects Tissue Engineering Tissue Scaffolds |
| title | Degradable Poly(amidoamine) Hydrogels as Scaffolds for In Vitro Culturing of Peripheral Nervous System Cells |
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