In vitro evaluation of hydroxyapatite-chitosan-gelatin composite membrane in guided tissue regeneration
Resorbable biomaterials have been investigated as barrier membranes to compartmentalize the periodontal defects while selectively guiding osteoprogenitor cell proliferation and bone tissue expansion. Hydroxyapatite (H), chitosan (C), and gelatin (G) have chemical similarity to the structural compone...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2013-04, Vol.101A (4), p.1016-1025 |
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| creator | Hunter, Kimberly T. Ma, Teng |
| description | Resorbable biomaterials have been investigated as barrier membranes to compartmentalize the periodontal defects while selectively guiding osteoprogenitor cell proliferation and bone tissue expansion. Hydroxyapatite (H), chitosan (C), and gelatin (G) have chemical similarity to the structural components of natural bone and their composites have been tested as bone scaffolds. Human mesenchymal stem or stromal cells (hMSCs) are inducible osteoprogenitors and are responsible for bone tissue repair and regeneration. In this study, the dynamic interactions of hMSC with composite hydroxyapatite–chitosan–gelatin (HCG) membranes were investigated. The association of HCG formed a biodegradable membrane with ∼60 wt % water and an initial stiffness of ∼20 kPa. Preconditioning in serum‐containing media resulted in the formation nanopores in the HCG membranes and the increase of extracellular matrix (ECM) protein adsorption. Expression of integrin α2β1 and α5β1 coincided with ECM enrichment, suggesting the enhanced cell–ECM interactions. The elevated expression of bone marker proteins and genes in the HCG membranes suggests the progression of hMSC osteogenic differentiation in the absence of chemical induction. The results showed that the HCG membranes possess sufficient mechanical and structural properties to function as a barrier membrane, and that the adsorbed ECM proteins effectively functionalized the HCG membranes and promoted hMSC osteogenic differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013. |
| doi_str_mv | 10.1002/jbm.a.34396 |
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Hydroxyapatite (H), chitosan (C), and gelatin (G) have chemical similarity to the structural components of natural bone and their composites have been tested as bone scaffolds. Human mesenchymal stem or stromal cells (hMSCs) are inducible osteoprogenitors and are responsible for bone tissue repair and regeneration. In this study, the dynamic interactions of hMSC with composite hydroxyapatite–chitosan–gelatin (HCG) membranes were investigated. The association of HCG formed a biodegradable membrane with ∼60 wt % water and an initial stiffness of ∼20 kPa. Preconditioning in serum‐containing media resulted in the formation nanopores in the HCG membranes and the increase of extracellular matrix (ECM) protein adsorption. Expression of integrin α2β1 and α5β1 coincided with ECM enrichment, suggesting the enhanced cell–ECM interactions. The elevated expression of bone marker proteins and genes in the HCG membranes suggests the progression of hMSC osteogenic differentiation in the absence of chemical induction. The results showed that the HCG membranes possess sufficient mechanical and structural properties to function as a barrier membrane, and that the adsorbed ECM proteins effectively functionalized the HCG membranes and promoted hMSC osteogenic differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.34396</identifier><identifier>PMID: 22968951</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adsorption ; barrier membrane ; Biocompatibility ; Biological and medical sciences ; Biomedical materials ; Bone Regeneration ; Bones ; Cell Differentiation ; Cells, Cultured ; chitosan ; Chitosan - chemistry ; composite scaffold ; Culture Media, Conditioned - pharmacology ; Differentiation ; Durapatite - chemistry ; Electrochemical machining ; Extracellular Matrix Proteins - metabolism ; Gelatin - chemistry ; guided bone regeneration ; guided tissue regeneration ; human mesenchymal stem cells ; Humans ; Hydroxyapatite ; Integrin alpha2beta1 - biosynthesis ; Integrin alpha5beta1 - biosynthesis ; Medical sciences ; Membranes ; Membranes, Artificial ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Orthopedic surgery ; Regeneration ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Resorbable biomaterials have been investigated as barrier membranes to compartmentalize the periodontal defects while selectively guiding osteoprogenitor cell proliferation and bone tissue expansion. Hydroxyapatite (H), chitosan (C), and gelatin (G) have chemical similarity to the structural components of natural bone and their composites have been tested as bone scaffolds. Human mesenchymal stem or stromal cells (hMSCs) are inducible osteoprogenitors and are responsible for bone tissue repair and regeneration. In this study, the dynamic interactions of hMSC with composite hydroxyapatite–chitosan–gelatin (HCG) membranes were investigated. The association of HCG formed a biodegradable membrane with ∼60 wt % water and an initial stiffness of ∼20 kPa. Preconditioning in serum‐containing media resulted in the formation nanopores in the HCG membranes and the increase of extracellular matrix (ECM) protein adsorption. Expression of integrin α2β1 and α5β1 coincided with ECM enrichment, suggesting the enhanced cell–ECM interactions. The elevated expression of bone marker proteins and genes in the HCG membranes suggests the progression of hMSC osteogenic differentiation in the absence of chemical induction. The results showed that the HCG membranes possess sufficient mechanical and structural properties to function as a barrier membrane, and that the adsorbed ECM proteins effectively functionalized the HCG membranes and promoted hMSC osteogenic differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.</description><subject>Adsorption</subject><subject>barrier membrane</subject><subject>Biocompatibility</subject><subject>Biological and medical sciences</subject><subject>Biomedical materials</subject><subject>Bone Regeneration</subject><subject>Bones</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>composite scaffold</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Differentiation</subject><subject>Durapatite - chemistry</subject><subject>Electrochemical machining</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Gelatin - chemistry</subject><subject>guided bone regeneration</subject><subject>guided tissue regeneration</subject><subject>human mesenchymal stem cells</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Integrin alpha2beta1 - biosynthesis</subject><subject>Integrin alpha5beta1 - biosynthesis</subject><subject>Medical sciences</subject><subject>Membranes</subject><subject>Membranes, Artificial</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Orthopedic surgery</subject><subject>Regeneration</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical implants</subject><subject>Technology. Biomaterials. Equipments</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1v1DAQxSMEoqVw4o58QUKqsnjs2I6PUNGlVfmSChwtx5lsXZJ4sZPS_e9xu9tyA062nn_zZsavKJ4DXQCl7PVlMyzsgldcywfFPgjBykpL8fDmXumSMy33iicpXWZYUsEeF3ssa7UWsF-sTkZy5acYCF7ZfraTDyMJHbnYtDFcb-w6KxOW7sJPIdmxXGGflZG4MKxDyk9kwKGJdkSS1dXsW2zJ5FOakURc4Yjx1vNp8aizfcJnu_Og-Hr87vzofXn2aXly9OasdIKDLBupHEpW1zVYhxUyycAClxpAAVOWdwp521aqBmw1RU07JkBS3UGjOXP8oHi19V3H8HPGNJnBJ4d9nycMczIgldK8kqL6D1RWjPJK83-jHJiUVEmV0cMt6mJIKWJn1tEPNm4MUHOTl8l5GWtu88r0i53x3AzY3rN3AWXg5Q6wydm-yz_tfPrDKZY3Ap052HK_fI-bv_U0p28_3DUvtzU-TXh9X2PjD5MXUcJ8_7g0NVfnyy-n38xn_huVHLyO</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>Hunter, Kimberly T.</creator><creator>Ma, Teng</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>201304</creationdate><title>In vitro evaluation of hydroxyapatite-chitosan-gelatin composite membrane in guided tissue regeneration</title><author>Hunter, Kimberly T. ; Ma, Teng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5316-b67ce628881ace4e2621a1369117127a3f7e3dd4781ed90e90f251609f1b932c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adsorption</topic><topic>barrier membrane</topic><topic>Biocompatibility</topic><topic>Biological and medical sciences</topic><topic>Biomedical materials</topic><topic>Bone Regeneration</topic><topic>Bones</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>composite scaffold</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Differentiation</topic><topic>Durapatite - chemistry</topic><topic>Electrochemical machining</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Gelatin - chemistry</topic><topic>guided bone regeneration</topic><topic>guided tissue regeneration</topic><topic>human mesenchymal stem cells</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Integrin alpha2beta1 - biosynthesis</topic><topic>Integrin alpha5beta1 - biosynthesis</topic><topic>Medical sciences</topic><topic>Membranes</topic><topic>Membranes, Artificial</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Orthopedic surgery</topic><topic>Regeneration</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical implants</topic><topic>Technology. Biomaterials. 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hunter, Kimberly T.</au><au>Ma, Teng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro evaluation of hydroxyapatite-chitosan-gelatin composite membrane in guided tissue regeneration</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2013-04</date><risdate>2013</risdate><volume>101A</volume><issue>4</issue><spage>1016</spage><epage>1025</epage><pages>1016-1025</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Resorbable biomaterials have been investigated as barrier membranes to compartmentalize the periodontal defects while selectively guiding osteoprogenitor cell proliferation and bone tissue expansion. Hydroxyapatite (H), chitosan (C), and gelatin (G) have chemical similarity to the structural components of natural bone and their composites have been tested as bone scaffolds. Human mesenchymal stem or stromal cells (hMSCs) are inducible osteoprogenitors and are responsible for bone tissue repair and regeneration. In this study, the dynamic interactions of hMSC with composite hydroxyapatite–chitosan–gelatin (HCG) membranes were investigated. The association of HCG formed a biodegradable membrane with ∼60 wt % water and an initial stiffness of ∼20 kPa. Preconditioning in serum‐containing media resulted in the formation nanopores in the HCG membranes and the increase of extracellular matrix (ECM) protein adsorption. Expression of integrin α2β1 and α5β1 coincided with ECM enrichment, suggesting the enhanced cell–ECM interactions. The elevated expression of bone marker proteins and genes in the HCG membranes suggests the progression of hMSC osteogenic differentiation in the absence of chemical induction. The results showed that the HCG membranes possess sufficient mechanical and structural properties to function as a barrier membrane, and that the adsorbed ECM proteins effectively functionalized the HCG membranes and promoted hMSC osteogenic differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22968951</pmid><doi>10.1002/jbm.a.34396</doi><tpages>10</tpages></addata></record> |
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| subjects | Adsorption barrier membrane Biocompatibility Biological and medical sciences Biomedical materials Bone Regeneration Bones Cell Differentiation Cells, Cultured chitosan Chitosan - chemistry composite scaffold Culture Media, Conditioned - pharmacology Differentiation Durapatite - chemistry Electrochemical machining Extracellular Matrix Proteins - metabolism Gelatin - chemistry guided bone regeneration guided tissue regeneration human mesenchymal stem cells Humans Hydroxyapatite Integrin alpha2beta1 - biosynthesis Integrin alpha5beta1 - biosynthesis Medical sciences Membranes Membranes, Artificial Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Orthopedic surgery Regeneration Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical implants Technology. Biomaterials. Equipments |
| title | In vitro evaluation of hydroxyapatite-chitosan-gelatin composite membrane in guided tissue regeneration |
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