Three-dimensional differential interferenrce contrast microscopy using synthetic aperture imaging
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image f...
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| Veröffentlicht in: | Journal of biomedical optics 2012-02, Vol.17 (2), p.026003-1-026003-2 |
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| container_end_page | 1-026003-2 |
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| container_issue | 2 |
| container_start_page | 026003 |
| container_title | Journal of biomedical optics |
| container_volume | 17 |
| creator | Kim, Moonseok Choi, Youngwoon Fang-Yen, Christopher Sung, Yongjin Kim, Kwanhyung Dasari, Ramachandra R Feld, Michael S Choi, Wonshik |
| description | We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent !mages. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles. |
| doi_str_mv | 10.1117/1.JBO.17.2.026003 |
| format | Article |
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| ispartof | Journal of biomedical optics, 2012-02, Vol.17 (2), p.026003-1-026003-2 |
| issn | 1083-3668 1560-2281 |
| language | eng |
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| source | PubMed; EZB Electronic Journals Library |
| subjects | Coherence Imaging Mathematical models Microscopy Propagation Synthetic apertures Three dimensional |
| title | Three-dimensional differential interferenrce contrast microscopy using synthetic aperture imaging |
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