GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum
The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lack...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 2015-03, Vol.37 (3), p.679-683 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 683 |
|---|---|
| container_issue | 3 |
| container_start_page | 679 |
| container_title | Biotechnology letters |
| container_volume | 37 |
| creator | Reyes-Medina, María Alejandra Macías-Sánchez, Karla Lizbeth |
| description | The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1, and rho1::hyg + rho1 ᴳ¹⁴ⱽ that has a constitutively active Rho1. The transcript levels of lcc2, lcc3, lcc5, and xyl3 differed among the three strains, but those of lcc1 and lcc9 did not. Xylanase activities were higher in rho1::hyg than in both the wild type and rho1::hyg + rho1 ᴳ¹⁴ⱽ . Laccase activities were significantly higher in the two mutants than in the wild type. Rho1 thus plays a role in regulating xyl3, lcc2, lcc3, and lcc5 at the transcriptional and/or translational level. |
| doi_str_mv | 10.1007/s10529-014-1709-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1677929212</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1664217325</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-bf970ce47c9dacbd895389b8b3f28b48931afab8ed2ff6a0e1c7d3e3138c80353</originalsourceid><addsrcrecordid>eNqNkUFv1DAQhS0EokvhB3ABS1y4BDx2HNtHVLUFqRIItmfLccbbVEm82Btp99_jKAUhDqiHkQ_zvTfjeYS8BvYBGFMfMzDJTcWgrkAxU5knZANSiapRqnlKNqUBlawNPyMvcr5njBnF1HNyxqWQoIFtyPZ6-81lpN_vItCEu3lwB8z0cIcUj_uEOfdxojHQ42kQ1E0dHZz3i2KHUwH7iV7N2aV-Hmk8nvI-pnl8SZ4FN2R89fCek9ury-3F5-rm6_WXi083lZeCH6o2lHU81sqbzvm200YKbVrdisB1W2sjwAXXaux4CI1jCF51AgUI7TUTUpyT96vvPsWfM-aDHfvscRjchHHOFsodDDcc-GNQwZTmtX4E2tQclODLAu_-Qe_jnKby50JJ3dRaNoshrJRPMeeEwe5TP7p0ssDsEqRdg7QlL7sEaU3RvHlwntsRuz-K38kVgK9ALq1ph-mv0f9xfbuKgovW7VKf7e0PzkCyUlKVO_0C6pKv9w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1658648568</pqid></control><display><type>article</type><title>GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Reyes-Medina, María Alejandra ; Macías-Sánchez, Karla Lizbeth</creator><creatorcontrib>Reyes-Medina, María Alejandra ; Macías-Sánchez, Karla Lizbeth</creatorcontrib><description>The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1, and rho1::hyg + rho1 ᴳ¹⁴ⱽ that has a constitutively active Rho1. The transcript levels of lcc2, lcc3, lcc5, and xyl3 differed among the three strains, but those of lcc1 and lcc9 did not. Xylanase activities were higher in rho1::hyg than in both the wild type and rho1::hyg + rho1 ᴳ¹⁴ⱽ . Laccase activities were significantly higher in the two mutants than in the wild type. Rho1 thus plays a role in regulating xyl3, lcc2, lcc3, and lcc5 at the transcriptional and/or translational level.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-014-1709-9</identifier><identifier>PMID: 25351810</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Applied Microbiology ; Biochemistry ; biogenesis ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; cell walls ; Enzymes ; Fungi ; Fusarium - genetics ; Fusarium - metabolism ; Fusarium oxysporum ; Fusarium oxysporum f. sp. lycopersici ; Gene Deletion ; Gene Expression ; Gene Expression Profiling ; Gene Expression Regulation, Fungal ; Genes ; guanosinetriphosphatase ; Laccase ; Laccase - metabolism ; Life Sciences ; Microbiology ; mutants ; Original Research Paper ; Plant pathology ; Proteins ; rho GTP-Binding Proteins - genetics ; rho GTP-Binding Proteins - metabolism ; Strain ; transcription (genetics) ; Xylanase ; xylanases ; Xylosidases - metabolism</subject><ispartof>Biotechnology letters, 2015-03, Vol.37 (3), p.679-683</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-bf970ce47c9dacbd895389b8b3f28b48931afab8ed2ff6a0e1c7d3e3138c80353</citedby><cites>FETCH-LOGICAL-c532t-bf970ce47c9dacbd895389b8b3f28b48931afab8ed2ff6a0e1c7d3e3138c80353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-014-1709-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-014-1709-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25351810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyes-Medina, María Alejandra</creatorcontrib><creatorcontrib>Macías-Sánchez, Karla Lizbeth</creatorcontrib><title>GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1, and rho1::hyg + rho1 ᴳ¹⁴ⱽ that has a constitutively active Rho1. The transcript levels of lcc2, lcc3, lcc5, and xyl3 differed among the three strains, but those of lcc1 and lcc9 did not. Xylanase activities were higher in rho1::hyg than in both the wild type and rho1::hyg + rho1 ᴳ¹⁴ⱽ . Laccase activities were significantly higher in the two mutants than in the wild type. Rho1 thus plays a role in regulating xyl3, lcc2, lcc3, and lcc5 at the transcriptional and/or translational level.</description><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>biogenesis</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>cell walls</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Fusarium - genetics</subject><subject>Fusarium - metabolism</subject><subject>Fusarium oxysporum</subject><subject>Fusarium oxysporum f. sp. lycopersici</subject><subject>Gene Deletion</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes</subject><subject>guanosinetriphosphatase</subject><subject>Laccase</subject><subject>Laccase - metabolism</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>mutants</subject><subject>Original Research Paper</subject><subject>Plant pathology</subject><subject>Proteins</subject><subject>rho GTP-Binding Proteins - genetics</subject><subject>rho GTP-Binding Proteins - metabolism</subject><subject>Strain</subject><subject>transcription (genetics)</subject><subject>Xylanase</subject><subject>xylanases</subject><subject>Xylosidases - metabolism</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkUFv1DAQhS0EokvhB3ABS1y4BDx2HNtHVLUFqRIItmfLccbbVEm82Btp99_jKAUhDqiHkQ_zvTfjeYS8BvYBGFMfMzDJTcWgrkAxU5knZANSiapRqnlKNqUBlawNPyMvcr5njBnF1HNyxqWQoIFtyPZ6-81lpN_vItCEu3lwB8z0cIcUj_uEOfdxojHQ42kQ1E0dHZz3i2KHUwH7iV7N2aV-Hmk8nvI-pnl8SZ4FN2R89fCek9ury-3F5-rm6_WXi083lZeCH6o2lHU81sqbzvm200YKbVrdisB1W2sjwAXXaux4CI1jCF51AgUI7TUTUpyT96vvPsWfM-aDHfvscRjchHHOFsodDDcc-GNQwZTmtX4E2tQclODLAu_-Qe_jnKby50JJ3dRaNoshrJRPMeeEwe5TP7p0ssDsEqRdg7QlL7sEaU3RvHlwntsRuz-K38kVgK9ALq1ph-mv0f9xfbuKgovW7VKf7e0PzkCyUlKVO_0C6pKv9w</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Reyes-Medina, María Alejandra</creator><creator>Macías-Sánchez, Karla Lizbeth</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7QO</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20150301</creationdate><title>GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum</title><author>Reyes-Medina, María Alejandra ; Macías-Sánchez, Karla Lizbeth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-bf970ce47c9dacbd895389b8b3f28b48931afab8ed2ff6a0e1c7d3e3138c80353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>biogenesis</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>cell walls</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Fusarium - genetics</topic><topic>Fusarium - metabolism</topic><topic>Fusarium oxysporum</topic><topic>Fusarium oxysporum f. sp. lycopersici</topic><topic>Gene Deletion</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes</topic><topic>guanosinetriphosphatase</topic><topic>Laccase</topic><topic>Laccase - metabolism</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>mutants</topic><topic>Original Research Paper</topic><topic>Plant pathology</topic><topic>Proteins</topic><topic>rho GTP-Binding Proteins - genetics</topic><topic>rho GTP-Binding Proteins - metabolism</topic><topic>Strain</topic><topic>transcription (genetics)</topic><topic>Xylanase</topic><topic>xylanases</topic><topic>Xylosidases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reyes-Medina, María Alejandra</creatorcontrib><creatorcontrib>Macías-Sánchez, Karla Lizbeth</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyes-Medina, María Alejandra</au><au>Macías-Sánchez, Karla Lizbeth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>37</volume><issue>3</issue><spage>679</spage><epage>683</epage><pages>679-683</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1, and rho1::hyg + rho1 ᴳ¹⁴ⱽ that has a constitutively active Rho1. The transcript levels of lcc2, lcc3, lcc5, and xyl3 differed among the three strains, but those of lcc1 and lcc9 did not. Xylanase activities were higher in rho1::hyg than in both the wild type and rho1::hyg + rho1 ᴳ¹⁴ⱽ . Laccase activities were significantly higher in the two mutants than in the wild type. Rho1 thus plays a role in regulating xyl3, lcc2, lcc3, and lcc5 at the transcriptional and/or translational level.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>25351810</pmid><doi>10.1007/s10529-014-1709-9</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2015-03, Vol.37 (3), p.679-683 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1677929212 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Applied Microbiology Biochemistry biogenesis Biomedical and Life Sciences Biosynthesis Biotechnology cell walls Enzymes Fungi Fusarium - genetics Fusarium - metabolism Fusarium oxysporum Fusarium oxysporum f. sp. lycopersici Gene Deletion Gene Expression Gene Expression Profiling Gene Expression Regulation, Fungal Genes guanosinetriphosphatase Laccase Laccase - metabolism Life Sciences Microbiology mutants Original Research Paper Plant pathology Proteins rho GTP-Binding Proteins - genetics rho GTP-Binding Proteins - metabolism Strain transcription (genetics) Xylanase xylanases Xylosidases - metabolism |
| title | GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T00%3A11%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GTPase%20Rho1%20regulates%20the%20expression%20of%20xyl3%20and%20laccase%20genes%20in%20Fusarium%20oxysporum&rft.jtitle=Biotechnology%20letters&rft.au=Reyes-Medina,%20Mar%C3%ADa%20Alejandra&rft.date=2015-03-01&rft.volume=37&rft.issue=3&rft.spage=679&rft.epage=683&rft.pages=679-683&rft.issn=0141-5492&rft.eissn=1573-6776&rft_id=info:doi/10.1007/s10529-014-1709-9&rft_dat=%3Cproquest_cross%3E1664217325%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1658648568&rft_id=info:pmid/25351810&rfr_iscdi=true |