Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model
A biodegradable fibrous tube was fabricated by electrospinning method using a combination of Poly(lactic‐co‐glycolic acid) (PLGA) and gelatin dissolved in trifluoroethanol (TFE). Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2013-08, Vol.101B (6), p.1095-1105 |
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| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
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| creator | Son, So-Ra Franco, Rose-Ann Bae, Sang-Ho Min, Young-Ki Lee, Byong-Taek |
| description | A biodegradable fibrous tube was fabricated by electrospinning method using a combination of Poly(lactic‐co‐glycolic acid) (PLGA) and gelatin dissolved in trifluoroethanol (TFE). Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum condition appropriate for intestinal stent application. Fiber morphology was visualized and analyzed using a scanning electron microscope (SEM). Characterizations of physical properties were done according to its tensile strength, surface hydrophilicity, swelling ability, and biodegradability. Biocompatibility of the scaffolds was investigated in vitro using IEC‐18 (Rat intestinal epithelial cell). Cell proliferation was quantified using MTT assay and cell adhesion behavior was visualized by SEM and confocal laser scanning microscope. PLGA/Gelatin (5/5) was determined to have adequate material properties and sufficient in vitro biocompatibility. This was then implanted in a male Sprague‐Dawley rat for 14 days to determine in vivo behavior of the sample. Histological examination on the intestinal tissue surrounding the graft showed normal morphology comparable to non‐implanted intestine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013. |
| doi_str_mv | 10.1002/jbm.b.32923 |
| format | Article |
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Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum condition appropriate for intestinal stent application. Fiber morphology was visualized and analyzed using a scanning electron microscope (SEM). Characterizations of physical properties were done according to its tensile strength, surface hydrophilicity, swelling ability, and biodegradability. Biocompatibility of the scaffolds was investigated in vitro using IEC‐18 (Rat intestinal epithelial cell). Cell proliferation was quantified using MTT assay and cell adhesion behavior was visualized by SEM and confocal laser scanning microscope. PLGA/Gelatin (5/5) was determined to have adequate material properties and sufficient in vitro biocompatibility. This was then implanted in a male Sprague‐Dawley rat for 14 days to determine in vivo behavior of the sample. Histological examination on the intestinal tissue surrounding the graft showed normal morphology comparable to non‐implanted intestine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.32923</identifier><identifier>PMID: 23564699</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Absorbable Implants ; Anastomosis, Surgical ; Animals ; Biocompatibility ; Biocompatible Materials - chemistry ; Biodegradability ; biodegradable ; Biological and medical sciences ; Biomedical materials ; Cell Proliferation ; Electrospinning ; gelatin ; Gelatin - chemistry ; Gelatins ; In vitro testing ; Intestinal Mucosa - cytology ; intestinal stent ; Intestines - cytology ; Intestines - surgery ; Lactic Acid - chemistry ; Male ; Materials Testing ; Medical sciences ; Morphology ; PLGA ; Polyglycolic Acid - chemistry ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Rats ; Scanning electron microscopy ; Stents ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments</subject><ispartof>Journal of biomedical materials research. 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Part B, Applied biomaterials</title><addtitle>J. Biomed. Mater. Res</addtitle><description>A biodegradable fibrous tube was fabricated by electrospinning method using a combination of Poly(lactic‐co‐glycolic acid) (PLGA) and gelatin dissolved in trifluoroethanol (TFE). Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum condition appropriate for intestinal stent application. Fiber morphology was visualized and analyzed using a scanning electron microscope (SEM). Characterizations of physical properties were done according to its tensile strength, surface hydrophilicity, swelling ability, and biodegradability. Biocompatibility of the scaffolds was investigated in vitro using IEC‐18 (Rat intestinal epithelial cell). Cell proliferation was quantified using MTT assay and cell adhesion behavior was visualized by SEM and confocal laser scanning microscope. PLGA/Gelatin (5/5) was determined to have adequate material properties and sufficient in vitro biocompatibility. This was then implanted in a male Sprague‐Dawley rat for 14 days to determine in vivo behavior of the sample. Histological examination on the intestinal tissue surrounding the graft showed normal morphology comparable to non‐implanted intestine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.</description><subject>Absorbable Implants</subject><subject>Anastomosis, Surgical</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biodegradability</subject><subject>biodegradable</subject><subject>Biological and medical sciences</subject><subject>Biomedical materials</subject><subject>Cell Proliferation</subject><subject>Electrospinning</subject><subject>gelatin</subject><subject>Gelatin - chemistry</subject><subject>Gelatins</subject><subject>In vitro testing</subject><subject>Intestinal Mucosa - cytology</subject><subject>intestinal stent</subject><subject>Intestines - cytology</subject><subject>Intestines - surgery</subject><subject>Lactic Acid - chemistry</subject><subject>Male</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Morphology</subject><subject>PLGA</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Rats</subject><subject>Scanning electron microscopy</subject><subject>Stents</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical implants</subject><subject>Technology. Biomaterials. Equipments</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0c9vFCEUB_CJ0dgfevJuuJiYmNkCD4bl2NZ21a4_mmg8EhigUpkfwkxq_3upu11v6glCPu8B71tVzwheEIzp0bXpFmYBVFJ4UO0TzmnN5JI83O0F7FUHOV8X3GAOj6s9CrxhjZT7VTiLrp3SkMe5R5_Wq-OjKxf1FHrkg0nDnNE0G5eRHxKavjmkxzGGtoChR4NHJgzWXSVttYkOhX5yudTqiPLk-qkcoKQn1BUUn1SPvI7ZPd2uh9WX87PPp2_q9cfV29Pjdd2yhkPtBeMOuLWMWMK9FgR7jltppCaCCCoJEd4AEOOodrAklC-ptVxbAMawhMPq5abvmIYfc3mP6kJuXYy6d-U_ijRCSMoxwH_QhlGMG8r_TUFKCXTJaaGvNrQtY83JeTWm0Ol0qwhWd4mpkpgy6ndiRT_fNp5N5-zO3kdUwIst0LnV0SfdtyH_caIhZSZ3jmzcTYju9m93qncn7-8vrzc1oeT1c1ej03fVCBBcff2wUpcXry8u2epcncAv5am8Dg</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Son, So-Ra</creator><creator>Franco, Rose-Ann</creator><creator>Bae, Sang-Ho</creator><creator>Min, Young-Ki</creator><creator>Lee, Byong-Taek</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>KR7</scope><scope>L7M</scope></search><sort><creationdate>201308</creationdate><title>Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model</title><author>Son, So-Ra ; Franco, Rose-Ann ; Bae, Sang-Ho ; Min, Young-Ki ; Lee, Byong-Taek</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4653-f745e35dd41d15fa710f50c9b9a171729117fb331be2ae3812582dd5ad3344093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Absorbable Implants</topic><topic>Anastomosis, Surgical</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biodegradability</topic><topic>biodegradable</topic><topic>Biological and medical sciences</topic><topic>Biomedical materials</topic><topic>Cell Proliferation</topic><topic>Electrospinning</topic><topic>gelatin</topic><topic>Gelatin - chemistry</topic><topic>Gelatins</topic><topic>In vitro testing</topic><topic>Intestinal Mucosa - cytology</topic><topic>intestinal stent</topic><topic>Intestines - cytology</topic><topic>Intestines - surgery</topic><topic>Lactic Acid - chemistry</topic><topic>Male</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Morphology</topic><topic>PLGA</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Rats</topic><topic>Scanning electron microscopy</topic><topic>Stents</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical implants</topic><topic>Technology. Biomaterials. Equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Son, So-Ra</creatorcontrib><creatorcontrib>Franco, Rose-Ann</creatorcontrib><creatorcontrib>Bae, Sang-Ho</creatorcontrib><creatorcontrib>Min, Young-Ki</creatorcontrib><creatorcontrib>Lee, Byong-Taek</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Son, So-Ra</au><au>Franco, Rose-Ann</au><au>Bae, Sang-Ho</au><au>Min, Young-Ki</au><au>Lee, Byong-Taek</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2013-08</date><risdate>2013</risdate><volume>101B</volume><issue>6</issue><spage>1095</spage><epage>1105</epage><pages>1095-1105</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>A biodegradable fibrous tube was fabricated by electrospinning method using a combination of Poly(lactic‐co‐glycolic acid) (PLGA) and gelatin dissolved in trifluoroethanol (TFE). Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum condition appropriate for intestinal stent application. Fiber morphology was visualized and analyzed using a scanning electron microscope (SEM). Characterizations of physical properties were done according to its tensile strength, surface hydrophilicity, swelling ability, and biodegradability. Biocompatibility of the scaffolds was investigated in vitro using IEC‐18 (Rat intestinal epithelial cell). Cell proliferation was quantified using MTT assay and cell adhesion behavior was visualized by SEM and confocal laser scanning microscope. PLGA/Gelatin (5/5) was determined to have adequate material properties and sufficient in vitro biocompatibility. This was then implanted in a male Sprague‐Dawley rat for 14 days to determine in vivo behavior of the sample. Histological examination on the intestinal tissue surrounding the graft showed normal morphology comparable to non‐implanted intestine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>23564699</pmid><doi>10.1002/jbm.b.32923</doi><tpages>11</tpages></addata></record> |
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| subjects | Absorbable Implants Anastomosis, Surgical Animals Biocompatibility Biocompatible Materials - chemistry Biodegradability biodegradable Biological and medical sciences Biomedical materials Cell Proliferation Electrospinning gelatin Gelatin - chemistry Gelatins In vitro testing Intestinal Mucosa - cytology intestinal stent Intestines - cytology Intestines - surgery Lactic Acid - chemistry Male Materials Testing Medical sciences Morphology PLGA Polyglycolic Acid - chemistry Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Rats Scanning electron microscopy Stents Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical implants Technology. Biomaterials. Equipments |
| title | Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model |
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