Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration
Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavi...
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Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2014-01, Vol.102 (1), p.42-51 |
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creator | Zeng, Qiongyu Han, Yan Li, Haiyan Chang, Jiang |
description | Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications. |
doi_str_mv | 10.1002/jbm.b.32978 |
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In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.32978</identifier><identifier>PMID: 23847006</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>3T3 Cells ; alginate ; Alginates ; Alkaline Phosphatase - metabolism ; Animals ; Beads ; bioactive glass ; Biocompatible Materials - chemistry ; Biological and medical sciences ; Biomedical materials ; Biotechnology ; Bone Regeneration ; Bones ; Carriers ; cell carrier ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Cell Transplantation ; Ceramics ; Current carriers ; Fundamental and applied biological sciences. Psychology ; Glass ; Glucuronic Acid ; Health. Pharmaceutical industry ; Hexuronic Acids ; Human Umbilical Vein Endothelial Cells ; Humans ; hydrogel ; Hydrogels ; Industrial applications and implications. Economical aspects ; Materials research ; Materials science ; Materials Testing ; Medical sciences ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Mice ; Miscellaneous ; Neovascularization, Physiologic ; Orthopedic surgery ; Osteogenesis ; Rats ; Regeneration ; Releasing ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments ; Tissue Engineering</subject><ispartof>Journal of biomedical materials research. Part B, Applied biomaterials, 2014-01, Vol.102 (1), p.42-51</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-486ee6f28590409af5e4cee8a04abddb270f7aaa2e8aac2a5dbe940f5e78effc3</citedby><cites>FETCH-LOGICAL-c554t-486ee6f28590409af5e4cee8a04abddb270f7aaa2e8aac2a5dbe940f5e78effc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28254147$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23847006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Qiongyu</creatorcontrib><creatorcontrib>Han, Yan</creatorcontrib><creatorcontrib>Li, Haiyan</creatorcontrib><creatorcontrib>Chang, Jiang</creatorcontrib><title>Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration</title><title>Journal of biomedical materials research. Part B, Applied biomaterials</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.</description><subject>3T3 Cells</subject><subject>alginate</subject><subject>Alginates</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Beads</subject><subject>bioactive glass</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical materials</subject><subject>Biotechnology</subject><subject>Bone Regeneration</subject><subject>Bones</subject><subject>Carriers</subject><subject>cell carrier</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Cell Transplantation</subject><subject>Ceramics</subject><subject>Current carriers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glass</subject><subject>Glucuronic Acid</subject><subject>Health. Pharmaceutical industry</subject><subject>Hexuronic Acids</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>Neovascularization, Physiologic</subject><subject>Orthopedic surgery</subject><subject>Osteogenesis</subject><subject>Rats</subject><subject>Regeneration</subject><subject>Releasing</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical implants</subject><subject>Technology. Biomaterials. 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Psychology</topic><topic>Glass</topic><topic>Glucuronic Acid</topic><topic>Health. Pharmaceutical industry</topic><topic>Hexuronic Acids</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>Neovascularization, Physiologic</topic><topic>Orthopedic surgery</topic><topic>Osteogenesis</topic><topic>Rats</topic><topic>Regeneration</topic><topic>Releasing</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical implants</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Qiongyu</creatorcontrib><creatorcontrib>Han, Yan</creatorcontrib><creatorcontrib>Li, Haiyan</creatorcontrib><creatorcontrib>Chang, Jiang</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Qiongyu</au><au>Han, Yan</au><au>Li, Haiyan</au><au>Chang, Jiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>102</volume><issue>1</issue><spage>42</spage><epage>51</epage><pages>42-51</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>23847006</pmid><doi>10.1002/jbm.b.32978</doi><tpages>10</tpages></addata></record> |
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subjects | 3T3 Cells alginate Alginates Alkaline Phosphatase - metabolism Animals Beads bioactive glass Biocompatible Materials - chemistry Biological and medical sciences Biomedical materials Biotechnology Bone Regeneration Bones Carriers cell carrier Cell Differentiation Cell Proliferation Cell Survival Cell Transplantation Ceramics Current carriers Fundamental and applied biological sciences. Psychology Glass Glucuronic Acid Health. Pharmaceutical industry Hexuronic Acids Human Umbilical Vein Endothelial Cells Humans hydrogel Hydrogels Industrial applications and implications. Economical aspects Materials research Materials science Materials Testing Medical sciences Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Mice Miscellaneous Neovascularization, Physiologic Orthopedic surgery Osteogenesis Rats Regeneration Releasing Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical implants Technology. Biomaterials. Equipments Tissue Engineering |
title | Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration |
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