Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration

Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2014-01, Vol.102 (1), p.42-51
Hauptverfasser: Zeng, Qiongyu, Han, Yan, Li, Haiyan, Chang, Jiang
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 51
container_issue 1
container_start_page 42
container_title Journal of biomedical materials research. Part B, Applied biomaterials
container_volume 102
creator Zeng, Qiongyu
Han, Yan
Li, Haiyan
Chang, Jiang
description Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.
doi_str_mv 10.1002/jbm.b.32978
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1677914939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1664197511</sourcerecordid><originalsourceid>FETCH-LOGICAL-c554t-486ee6f28590409af5e4cee8a04abddb270f7aaa2e8aac2a5dbe940f5e78effc3</originalsourceid><addsrcrecordid>eNqN0c9rFDEUB_BBFFurJ-8yIIIgs83PSXKs1bbCUhUUj-El82bNOjNZk1mw_71Zd13Bi57ySD58yeNbVU8pWVBC2PnajQu34Mwofa86pVKyRhhN7x9nxU-qRzmvC26J5A-rE8a1UIS0p9XH1yGuBsj5HIZVmGDG2sdxE3Mo09e7LsUVDrVD6HINufY4DLWHlAKmXPcx1S5OWCdc4YQJ5hCnx9WDHoaMTw7nWfX56u2ny5tm-f763eXFsvFSirkRukVse6alIYIY6CUKj6iBCHBd55givQIAVq7AM5CdQyNIYUpj33t-Vr3c525S_L7FPNsx5N3_YMK4zZa2ShkqDDf_QVtBjZKU_ptKIoQURrBCn_9F13GbprLzLpBQIwnXRb3aK59izgl7u0lhhHRnKbG7_mzpzzr7q7-inx0yt27E7mh_F1bAiwOA7GHoE0w-5D9OMymoUMU1exfyjD-O75C-2VZxJe2X22urb8WbJf9wZW_4Tx5Eshc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1660195038</pqid></control><display><type>article</type><title>Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Zeng, Qiongyu ; Han, Yan ; Li, Haiyan ; Chang, Jiang</creator><creatorcontrib>Zeng, Qiongyu ; Han, Yan ; Li, Haiyan ; Chang, Jiang</creatorcontrib><description>Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.32978</identifier><identifier>PMID: 23847006</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>3T3 Cells ; alginate ; Alginates ; Alkaline Phosphatase - metabolism ; Animals ; Beads ; bioactive glass ; Biocompatible Materials - chemistry ; Biological and medical sciences ; Biomedical materials ; Biotechnology ; Bone Regeneration ; Bones ; Carriers ; cell carrier ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Cell Transplantation ; Ceramics ; Current carriers ; Fundamental and applied biological sciences. Psychology ; Glass ; Glucuronic Acid ; Health. Pharmaceutical industry ; Hexuronic Acids ; Human Umbilical Vein Endothelial Cells ; Humans ; hydrogel ; Hydrogels ; Industrial applications and implications. Economical aspects ; Materials research ; Materials science ; Materials Testing ; Medical sciences ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Mice ; Miscellaneous ; Neovascularization, Physiologic ; Orthopedic surgery ; Osteogenesis ; Rats ; Regeneration ; Releasing ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments ; Tissue Engineering</subject><ispartof>Journal of biomedical materials research. Part B, Applied biomaterials, 2014-01, Vol.102 (1), p.42-51</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-486ee6f28590409af5e4cee8a04abddb270f7aaa2e8aac2a5dbe940f5e78effc3</citedby><cites>FETCH-LOGICAL-c554t-486ee6f28590409af5e4cee8a04abddb270f7aaa2e8aac2a5dbe940f5e78effc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=28254147$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23847006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Qiongyu</creatorcontrib><creatorcontrib>Han, Yan</creatorcontrib><creatorcontrib>Li, Haiyan</creatorcontrib><creatorcontrib>Chang, Jiang</creatorcontrib><title>Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration</title><title>Journal of biomedical materials research. Part B, Applied biomaterials</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.</description><subject>3T3 Cells</subject><subject>alginate</subject><subject>Alginates</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Beads</subject><subject>bioactive glass</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical materials</subject><subject>Biotechnology</subject><subject>Bone Regeneration</subject><subject>Bones</subject><subject>Carriers</subject><subject>cell carrier</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Cell Transplantation</subject><subject>Ceramics</subject><subject>Current carriers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glass</subject><subject>Glucuronic Acid</subject><subject>Health. Pharmaceutical industry</subject><subject>Hexuronic Acids</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>Neovascularization, Physiologic</subject><subject>Orthopedic surgery</subject><subject>Osteogenesis</subject><subject>Rats</subject><subject>Regeneration</subject><subject>Releasing</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical implants</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue Engineering</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0c9rFDEUB_BBFFurJ-8yIIIgs83PSXKs1bbCUhUUj-El82bNOjNZk1mw_71Zd13Bi57ySD58yeNbVU8pWVBC2PnajQu34Mwofa86pVKyRhhN7x9nxU-qRzmvC26J5A-rE8a1UIS0p9XH1yGuBsj5HIZVmGDG2sdxE3Mo09e7LsUVDrVD6HINufY4DLWHlAKmXPcx1S5OWCdc4YQJ5hCnx9WDHoaMTw7nWfX56u2ny5tm-f763eXFsvFSirkRukVse6alIYIY6CUKj6iBCHBd55givQIAVq7AM5CdQyNIYUpj33t-Vr3c525S_L7FPNsx5N3_YMK4zZa2ShkqDDf_QVtBjZKU_ptKIoQURrBCn_9F13GbprLzLpBQIwnXRb3aK59izgl7u0lhhHRnKbG7_mzpzzr7q7-inx0yt27E7mh_F1bAiwOA7GHoE0w-5D9OMymoUMU1exfyjD-O75C-2VZxJe2X22urb8WbJf9wZW_4Tx5Eshc</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Zeng, Qiongyu</creator><creator>Han, Yan</creator><creator>Li, Haiyan</creator><creator>Chang, Jiang</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20140101</creationdate><title>Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration</title><author>Zeng, Qiongyu ; Han, Yan ; Li, Haiyan ; Chang, Jiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-486ee6f28590409af5e4cee8a04abddb270f7aaa2e8aac2a5dbe940f5e78effc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3T3 Cells</topic><topic>alginate</topic><topic>Alginates</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Beads</topic><topic>bioactive glass</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical materials</topic><topic>Biotechnology</topic><topic>Bone Regeneration</topic><topic>Bones</topic><topic>Carriers</topic><topic>cell carrier</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cell Survival</topic><topic>Cell Transplantation</topic><topic>Ceramics</topic><topic>Current carriers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glass</topic><topic>Glucuronic Acid</topic><topic>Health. Pharmaceutical industry</topic><topic>Hexuronic Acids</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>Neovascularization, Physiologic</topic><topic>Orthopedic surgery</topic><topic>Osteogenesis</topic><topic>Rats</topic><topic>Regeneration</topic><topic>Releasing</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical implants</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Qiongyu</creatorcontrib><creatorcontrib>Han, Yan</creatorcontrib><creatorcontrib>Li, Haiyan</creatorcontrib><creatorcontrib>Chang, Jiang</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts – Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Qiongyu</au><au>Han, Yan</au><au>Li, Haiyan</au><au>Chang, Jiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>102</volume><issue>1</issue><spage>42</spage><epage>51</epage><pages>42-51</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>23847006</pmid><doi>10.1002/jbm.b.32978</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1552-4973
ispartof Journal of biomedical materials research. Part B, Applied biomaterials, 2014-01, Vol.102 (1), p.42-51
issn 1552-4973
1552-4981
language eng
recordid cdi_proquest_miscellaneous_1677914939
source MEDLINE; Wiley Online Library All Journals
subjects 3T3 Cells
alginate
Alginates
Alkaline Phosphatase - metabolism
Animals
Beads
bioactive glass
Biocompatible Materials - chemistry
Biological and medical sciences
Biomedical materials
Biotechnology
Bone Regeneration
Bones
Carriers
cell carrier
Cell Differentiation
Cell Proliferation
Cell Survival
Cell Transplantation
Ceramics
Current carriers
Fundamental and applied biological sciences. Psychology
Glass
Glucuronic Acid
Health. Pharmaceutical industry
Hexuronic Acids
Human Umbilical Vein Endothelial Cells
Humans
hydrogel
Hydrogels
Industrial applications and implications. Economical aspects
Materials research
Materials science
Materials Testing
Medical sciences
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Mice
Miscellaneous
Neovascularization, Physiologic
Orthopedic surgery
Osteogenesis
Rats
Regeneration
Releasing
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgical implants
Technology. Biomaterials. Equipments
Tissue Engineering
title Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T16%3A49%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bioglass/alginate%20composite%20hydrogel%20beads%20as%20cell%20carriers%20for%20bone%20regeneration&rft.jtitle=Journal%20of%20biomedical%20materials%20research.%20Part%20B,%20Applied%20biomaterials&rft.au=Zeng,%20Qiongyu&rft.date=2014-01-01&rft.volume=102&rft.issue=1&rft.spage=42&rft.epage=51&rft.pages=42-51&rft.issn=1552-4973&rft.eissn=1552-4981&rft_id=info:doi/10.1002/jbm.b.32978&rft_dat=%3Cproquest_cross%3E1664197511%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1660195038&rft_id=info:pmid/23847006&rfr_iscdi=true