Trimethylpsoralen Induces Small Deletion Mutations in Caenorhabditis elegans
To examine the mutagenic spectrum of 4,5',8-trimethylpsoralen (TMP) in Caenorhabditis elegans, we isolated mutations in the unc-22 and pal-1 genes following TMP mutagenesis and analyzed them for restriction fragment length polymorphisms by Southern blot. Eleven of 21 unc-22 mutations exhibited...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-02, Vol.91 (4), p.1381-1385 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Yandell, Mark D. Edgar, Lois G. Wood, William B. |
| description | To examine the mutagenic spectrum of 4,5',8-trimethylpsoralen (TMP) in Caenorhabditis elegans, we isolated mutations in the unc-22 and pal-1 genes following TMP mutagenesis and analyzed them for restriction fragment length polymorphisms by Southern blot. Eleven of 21 unc-22 mutations exhibited restriction fragment length polymorphisms, 8 of which were deletions of between 0.10 and 15 kb in length. Both of two pal-1 mutations were also small deletions within this size range. Comparison of our results with previous studies on mutagenesis by γ-rays and x-rays suggests that the mutagenic spectrum of TMP may be similar. TMP should be useful in generating mutations that cause complete loss of function of single genes and that are likely to result in allele-specific DNA polymorphisms. |
| doi_str_mv | 10.1073/pnas.91.4.1381 |
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Eleven of 21 unc-22 mutations exhibited restriction fragment length polymorphisms, 8 of which were deletions of between 0.10 and 15 kb in length. Both of two pal-1 mutations were also small deletions within this size range. Comparison of our results with previous studies on mutagenesis by γ-rays and x-rays suggests that the mutagenic spectrum of TMP may be similar. TMP should be useful in generating mutations that cause complete loss of function of single genes and that are likely to result in allele-specific DNA polymorphisms.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.4.1381</identifier><identifier>PMID: 7906415</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Alleles ; Animals ; Biological and medical sciences ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans Proteins ; Calmodulin-Binding Proteins ; Classical genetics, quantitative genetics, hybrids ; DNA ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Helminth - genetics ; Genetic loci ; Genetic mutation ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genomics ; Helminth Proteins - genetics ; Homeodomain Proteins ; Invertebrata ; Ionizing radiation ; Muscle Proteins - genetics ; Mutagenesis ; Mutagens ; Mutagens - pharmacology ; Mutation ; Point mutation ; Polymorphism, Restriction Fragment Length ; Sequence Deletion ; Trans-Activators ; Trioxsalen - pharmacology ; Worms</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-02, Vol.91 (4), p.1381-1385</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>1994 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Feb 15, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2364104$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2364104$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4168417$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7906415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yandell, Mark D.</creatorcontrib><creatorcontrib>Edgar, Lois G.</creatorcontrib><creatorcontrib>Wood, William B.</creatorcontrib><title>Trimethylpsoralen Induces Small Deletion Mutations in Caenorhabditis elegans</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>To examine the mutagenic spectrum of 4,5',8-trimethylpsoralen (TMP) in Caenorhabditis elegans, we isolated mutations in the unc-22 and pal-1 genes following TMP mutagenesis and analyzed them for restriction fragment length polymorphisms by Southern blot. Eleven of 21 unc-22 mutations exhibited restriction fragment length polymorphisms, 8 of which were deletions of between 0.10 and 15 kb in length. Both of two pal-1 mutations were also small deletions within this size range. Comparison of our results with previous studies on mutagenesis by γ-rays and x-rays suggests that the mutagenic spectrum of TMP may be similar. TMP should be useful in generating mutations that cause complete loss of function of single genes and that are likely to result in allele-specific DNA polymorphisms.</description><subject>Alleles</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans Proteins</subject><subject>Calmodulin-Binding Proteins</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Helminth - genetics</subject><subject>Genetic loci</subject><subject>Genetic mutation</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomics</subject><subject>Helminth Proteins - genetics</subject><subject>Homeodomain Proteins</subject><subject>Invertebrata</subject><subject>Ionizing radiation</subject><subject>Muscle Proteins - genetics</subject><subject>Mutagenesis</subject><subject>Mutagens</subject><subject>Mutagens - pharmacology</subject><subject>Mutation</subject><subject>Point mutation</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Sequence Deletion</subject><subject>Trans-Activators</subject><subject>Trioxsalen - pharmacology</subject><subject>Worms</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc-LEzEUx4Moa129elIYRLxNzcuk-QF7kfproeLB9Rwy6ZttSprUSUbc_97ULaWKpwQ-n5d833uEPAc6Byq7t_to81zDnM-hU_CAzIBqaAXX9CGZUcpkqzjjj8mTnLeUUr1Q9IJcSE0Fh8WMrG5Gv8OyuQv7nEYbMDbXcT05zM23nQ2heY8Bi0-x-TIVe7jkxsdmaTGmcWP7tS8-N9W5tTE_JY8GGzI-O56X5PvHDzfLz-3q66fr5btVu-WMllZbV6MyKux6WAhFUUq9AC5QMegZSqeVoMqxXroeey1YJ5CBZoPoNVfIuktydf_ufup3uHYYS41u9rUVO96ZZL35m0S_Mbfpp-EdiEP5m2P5mH5MmIvZ-ewwBBsxTdmAkFKwP-Krf8RtmsZYWzOMQse01rRKL8_DnFIcR1z56yO32dkwjDY6n08aB6E4yLNQdaEnqsFwc1isGaYQCv4qZ__9V6z8xT3f5pLGk1CHyIHy7jdCZKvI</recordid><startdate>19940215</startdate><enddate>19940215</enddate><creator>Yandell, Mark D.</creator><creator>Edgar, Lois G.</creator><creator>Wood, William B.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7U7</scope><scope>5PM</scope></search><sort><creationdate>19940215</creationdate><title>Trimethylpsoralen Induces Small Deletion Mutations in Caenorhabditis elegans</title><author>Yandell, Mark D. ; Edgar, Lois G. ; Wood, William B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j420t-9ac381206adf5680e7795146e821b2e7c98608c2b7cbeb96236e2192f6b948e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans Proteins</topic><topic>Calmodulin-Binding Proteins</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Helminth - genetics</topic><topic>Genetic loci</topic><topic>Genetic mutation</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. 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| subjects | Alleles Animals Biological and medical sciences Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins Calmodulin-Binding Proteins Classical genetics, quantitative genetics, hybrids DNA Fundamental and applied biological sciences. Psychology Genes Genes, Helminth - genetics Genetic loci Genetic mutation Genetics Genetics of eukaryotes. Biological and molecular evolution Genomics Helminth Proteins - genetics Homeodomain Proteins Invertebrata Ionizing radiation Muscle Proteins - genetics Mutagenesis Mutagens Mutagens - pharmacology Mutation Point mutation Polymorphism, Restriction Fragment Length Sequence Deletion Trans-Activators Trioxsalen - pharmacology Worms |
| title | Trimethylpsoralen Induces Small Deletion Mutations in Caenorhabditis elegans |
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