Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci
Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of...
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| Veröffentlicht in: | Theoretical and applied genetics 1994-11, Vol.89 (5), p.606-614 |
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| creator | Cai, Q Guy, C.L Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program) |
| description | Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x (Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.). A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning three loci were mapped detected by a cold-acclimation-responsive cDNA. |
| doi_str_mv | 10.1007/bf00222455 |
| format | Article |
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(Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</creator><creatorcontrib>Cai, Q ; Guy, C.L ; Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</creatorcontrib><description>Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x (Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.). A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning three loci were mapped detected by a cold-acclimation-responsive cDNA.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/bf00222455</identifier><identifier>PMID: 24177937</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>acclimation ; ADN ; Biological and medical sciences ; CARTE GENETIQUE ; chromosome mapping ; CITRUS GRANDIS ; Citrus maxima ; Classical genetics, quantitative genetics, hybrids ; cold ; ENZIMA DE RESTRICCION ; ENZYME DE RESTRICTION ; FRIO ; FROID ; Fruit breeding ; Fundamental and applied biological sciences. Psychology ; Genetic map ; genetic markers ; Genetics of eukaryotes. Biological and molecular evolution ; HIBRIDACION INTERGENERICA ; HYBRIDATION INTERGENERIQUE ; intergeneric hybridization ; linkage groups ; loci ; MAPAS GENETICOS ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; Molecular markers ; Pflanzenzuechtung ; POLIMORFISMO GENETICO ; POLYMORPHISME GENETIQUE ; PONCIRUS TRIFOLIATA ; Pteridophyta, spermatophyta ; RAPD ; RESISTANCE A LA TEMPERATURE ; RESISTENCIA A LA TEMPERATURA ; restriction fragment length polymorphism ; RFLP ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; TOLERANCE AU SEL ; TOLERANCIA A LA SAL ; Vegetals</subject><ispartof>Theoretical and applied genetics, 1994-11, Vol.89 (5), p.606-614</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-35b2da396427a9824f79203667d29a92319fa8cd9236dfce1126e67a350c72a63</citedby><cites>FETCH-LOGICAL-c454t-35b2da396427a9824f79203667d29a92319fa8cd9236dfce1126e67a350c72a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3314256$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24177937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Q</creatorcontrib><creatorcontrib>Guy, C.L</creatorcontrib><creatorcontrib>Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</creatorcontrib><title>Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x (Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.). A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning three loci were mapped detected by a cold-acclimation-responsive cDNA.</description><subject>acclimation</subject><subject>ADN</subject><subject>Biological and medical sciences</subject><subject>CARTE GENETIQUE</subject><subject>chromosome mapping</subject><subject>CITRUS GRANDIS</subject><subject>Citrus maxima</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>cold</subject><subject>ENZIMA DE RESTRICCION</subject><subject>ENZYME DE RESTRICTION</subject><subject>FRIO</subject><subject>FROID</subject><subject>Fruit breeding</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic map</subject><subject>genetic markers</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>HIBRIDACION INTERGENERICA</subject><subject>HYBRIDATION INTERGENERIQUE</subject><subject>intergeneric hybridization</subject><subject>linkage groups</subject><subject>loci</subject><subject>MAPAS GENETICOS</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>Molecular markers</subject><subject>Pflanzenzuechtung</subject><subject>POLIMORFISMO GENETICO</subject><subject>POLYMORPHISME GENETIQUE</subject><subject>PONCIRUS TRIFOLIATA</subject><subject>Pteridophyta, spermatophyta</subject><subject>RAPD</subject><subject>RESISTANCE A LA TEMPERATURE</subject><subject>RESISTENCIA A LA TEMPERATURA</subject><subject>restriction fragment length polymorphism</subject><subject>RFLP</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>TOLERANCE AU SEL</subject><subject>TOLERANCIA A LA SAL</subject><subject>Vegetals</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp90c9vFCEUB3BiNHbbevHoQTkYU01G4QHDzHG7P9RkU5tqzxOWgS12ZhhhprF_hv-xTHbbgwdPQPLh-3g8hF5S8pESIj9tLSEAwIV4gmaUM8jSAZ6iGSGcZEIKOELHMf4kiQnCnqMj4FTKkskZ-rP6PZguOt9hb_FwY3Djulu1M7hVPXYdXrghjBGP0XU7HFRX-xartm-cdabGvW_uWx_6G6fx8mKOz67ml8v36W64NSHixPHVenM5hfVTQKqhfVNnSuvGtWpIdbNgYu_TE-5Sba_dKXpmVRPNi8N6gq7Xqx-LL9nm2-evi_km01zwIWNiC7ViZc5BqrIAbmUJhOW5rKFUJTBaWlXoOu3y2mpDKeQml4oJoiWonJ2gd_vcPvhfo4lD1bqoTdOozvgxVjSXkhYFT_Ds_5DzAkAUTCT6YU918DEGY6s-pDbDfUVJNc2qOl8_zCrh14fccdua-pE-DCeBtwegolaNTb-vXXx0jFEOYmrkzZ5Z5Su1C4lcfwdCGaG8LCRMQa_-FctVmZ-T9DnsLwbOq5o</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Cai, Q</creator><creator>Guy, C.L</creator><creator>Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19941101</creationdate><title>Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci</title><author>Cai, Q ; Guy, C.L ; Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-35b2da396427a9824f79203667d29a92319fa8cd9236dfce1126e67a350c72a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>acclimation</topic><topic>ADN</topic><topic>Biological and medical sciences</topic><topic>CARTE GENETIQUE</topic><topic>chromosome mapping</topic><topic>CITRUS GRANDIS</topic><topic>Citrus maxima</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>cold</topic><topic>ENZIMA DE RESTRICCION</topic><topic>ENZYME DE RESTRICTION</topic><topic>FRIO</topic><topic>FROID</topic><topic>Fruit breeding</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic map</topic><topic>genetic markers</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>HIBRIDACION INTERGENERICA</topic><topic>HYBRIDATION INTERGENERIQUE</topic><topic>intergeneric hybridization</topic><topic>linkage groups</topic><topic>loci</topic><topic>MAPAS GENETICOS</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>Molecular markers</topic><topic>Pflanzenzuechtung</topic><topic>POLIMORFISMO GENETICO</topic><topic>POLYMORPHISME GENETIQUE</topic><topic>PONCIRUS TRIFOLIATA</topic><topic>Pteridophyta, spermatophyta</topic><topic>RAPD</topic><topic>RESISTANCE A LA TEMPERATURE</topic><topic>RESISTENCIA A LA TEMPERATURA</topic><topic>restriction fragment length polymorphism</topic><topic>RFLP</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>TOLERANCE AU SEL</topic><topic>TOLERANCIA A LA SAL</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Q</creatorcontrib><creatorcontrib>Guy, C.L</creatorcontrib><creatorcontrib>Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Q</au><au>Guy, C.L</au><au>Moore, G.A. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Dept. of Environmental and Horticultural Sciences. Plant Molecular and Cellular Biology Program)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>89</volume><issue>5</issue><spage>606</spage><epage>614</epage><pages>606-614</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x (Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.). A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning three loci were mapped detected by a cold-acclimation-responsive cDNA.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24177937</pmid><doi>10.1007/bf00222455</doi><tpages>9</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1994-11, Vol.89 (5), p.606-614 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | acclimation ADN Biological and medical sciences CARTE GENETIQUE chromosome mapping CITRUS GRANDIS Citrus maxima Classical genetics, quantitative genetics, hybrids cold ENZIMA DE RESTRICCION ENZYME DE RESTRICTION FRIO FROID Fruit breeding Fundamental and applied biological sciences. Psychology Genetic map genetic markers Genetics of eukaryotes. Biological and molecular evolution HIBRIDACION INTERGENERICA HYBRIDATION INTERGENERIQUE intergeneric hybridization linkage groups loci MAPAS GENETICOS MARCADORES GENETICOS MARQUEUR GENETIQUE Molecular markers Pflanzenzuechtung POLIMORFISMO GENETICO POLYMORPHISME GENETIQUE PONCIRUS TRIFOLIATA Pteridophyta, spermatophyta RAPD RESISTANCE A LA TEMPERATURE RESISTENCIA A LA TEMPERATURA restriction fragment length polymorphism RFLP SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE TOLERANCE AU SEL TOLERANCIA A LA SAL Vegetals |
| title | Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci |
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