Differentiation of tyrosinase and laccase using 4-hexyl-resorcinol, a tyrosinase inhibitor
Crude extracts of Agaricus bisporus and partially purified mushroom tyrosinase contained tyrosinase and laccase activities. 4-Hexylresorcinol (4HR) and salicylhydroxamic acid (SHAM) inhibited tyrosinase, but not laccase when selective substrates for tyrosinase and laccase were used in enzyme assays....
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| Veröffentlicht in: | Phytochemistry (Oxford) 1993-05, Vol.33 (2), p.281-284 |
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| container_title | Phytochemistry (Oxford) |
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| creator | Dawley, Ruthellen M. Flurkey, William H. |
| description | Crude extracts of
Agaricus bisporus and partially purified mushroom tyrosinase contained tyrosinase and laccase activities. 4-Hexylresorcinol (4HR) and salicylhydroxamic acid (SHAM) inhibited tyrosinase, but not laccase when selective substrates for tyrosinase and laccase were used in enzyme assays. Partially purified laccase from
Pyricularia oryzae did not exhibit tyrosinase activity, nor was it inhibited by 4HR or SHAM. Both 4HR and SHAM selectively inhibited tyrosinase isoforms separated by native electrophoresis. At low concentrations, 4HR was a selective inhibitor of tyrosinase and could differentiate between tyrosinase and laccase activities. |
| doi_str_mv | 10.1016/0031-9422(93)85503-J |
| format | Article |
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Agaricus bisporus and partially purified mushroom tyrosinase contained tyrosinase and laccase activities. 4-Hexylresorcinol (4HR) and salicylhydroxamic acid (SHAM) inhibited tyrosinase, but not laccase when selective substrates for tyrosinase and laccase were used in enzyme assays. Partially purified laccase from
Pyricularia oryzae did not exhibit tyrosinase activity, nor was it inhibited by 4HR or SHAM. Both 4HR and SHAM selectively inhibited tyrosinase isoforms separated by native electrophoresis. At low concentrations, 4HR was a selective inhibitor of tyrosinase and could differentiate between tyrosinase and laccase activities.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/0031-9422(93)85503-J</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>4-hexylresorcinol ; Agaricaceae ; Agaricus bisporus ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; differentiation ; Enzymes ; Fundamental and applied biological sciences. Psychology ; fungi ; Hyphomycetes ; inhibition ; laccase ; Metabolism ; Plant physiology and development ; Pyricularia oryzae ; tyrosinase</subject><ispartof>Phytochemistry (Oxford), 1993-05, Vol.33 (2), p.281-284</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-230847c7f3396978a3ba02e5591e5e09d697f62d2cd56be4c228cc3d561fc85d3</citedby><cites>FETCH-LOGICAL-c430t-230847c7f3396978a3ba02e5591e5e09d697f62d2cd56be4c228cc3d561fc85d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0031-9422(93)85503-J$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4845156$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Dawley, Ruthellen M.</creatorcontrib><creatorcontrib>Flurkey, William H.</creatorcontrib><title>Differentiation of tyrosinase and laccase using 4-hexyl-resorcinol, a tyrosinase inhibitor</title><title>Phytochemistry (Oxford)</title><description>Crude extracts of
Agaricus bisporus and partially purified mushroom tyrosinase contained tyrosinase and laccase activities. 4-Hexylresorcinol (4HR) and salicylhydroxamic acid (SHAM) inhibited tyrosinase, but not laccase when selective substrates for tyrosinase and laccase were used in enzyme assays. Partially purified laccase from
Pyricularia oryzae did not exhibit tyrosinase activity, nor was it inhibited by 4HR or SHAM. Both 4HR and SHAM selectively inhibited tyrosinase isoforms separated by native electrophoresis. At low concentrations, 4HR was a selective inhibitor of tyrosinase and could differentiate between tyrosinase and laccase activities.</description><subject>4-hexylresorcinol</subject><subject>Agaricaceae</subject><subject>Agaricus bisporus</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>differentiation</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungi</subject><subject>Hyphomycetes</subject><subject>inhibition</subject><subject>laccase</subject><subject>Metabolism</subject><subject>Plant physiology and development</subject><subject>Pyricularia oryzae</subject><subject>tyrosinase</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp9kLtOwzAUhi0EEqXwBgwZEAKJgB3HTrIgoXKtKrHAwmK5J8fUKLWLnSL69iS0qpiYzkXffy4_IceMXjLK5BWlnKVVnmVnFT8vhaA8He-QASsLnvKC0l0y2CL75CDGD0qpEFIOyNutNQYDutbq1nqXeJO0q-CjdTpiol2dNBqgz5dd7z3J0xl-r5o0YPQBrPPNRaL_Sqyb2altfTgke0Y3EY82cUhe7-9eRo_p5PnhaXQzSSHntE0zTsu8gMJwXsmqKDWfapqhEBVDgbSqu6aRWZ1BLeQUc8iyEoB3BTNQipoPyel67iL4zyXGVs1tBGwa7dAvo2KykEXByw7M1yB0x8aARi2CneuwUoyq3kjVu6R6l1TF1a-RatzJTjbzdQTdmKAd2LjV5mUumJAddr3GsPv1y2JQESw6wNoGhFbV3v6_5wfHNoeO</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Dawley, Ruthellen M.</creator><creator>Flurkey, William H.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>19930501</creationdate><title>Differentiation of tyrosinase and laccase using 4-hexyl-resorcinol, a tyrosinase inhibitor</title><author>Dawley, Ruthellen M. ; Flurkey, William H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-230847c7f3396978a3ba02e5591e5e09d697f62d2cd56be4c228cc3d561fc85d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>4-hexylresorcinol</topic><topic>Agaricaceae</topic><topic>Agaricus bisporus</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>differentiation</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungi</topic><topic>Hyphomycetes</topic><topic>inhibition</topic><topic>laccase</topic><topic>Metabolism</topic><topic>Plant physiology and development</topic><topic>Pyricularia oryzae</topic><topic>tyrosinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dawley, Ruthellen M.</creatorcontrib><creatorcontrib>Flurkey, William H.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dawley, Ruthellen M.</au><au>Flurkey, William H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of tyrosinase and laccase using 4-hexyl-resorcinol, a tyrosinase inhibitor</atitle><jtitle>Phytochemistry (Oxford)</jtitle><date>1993-05-01</date><risdate>1993</risdate><volume>33</volume><issue>2</issue><spage>281</spage><epage>284</epage><pages>281-284</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>Crude extracts of
Agaricus bisporus and partially purified mushroom tyrosinase contained tyrosinase and laccase activities. 4-Hexylresorcinol (4HR) and salicylhydroxamic acid (SHAM) inhibited tyrosinase, but not laccase when selective substrates for tyrosinase and laccase were used in enzyme assays. Partially purified laccase from
Pyricularia oryzae did not exhibit tyrosinase activity, nor was it inhibited by 4HR or SHAM. Both 4HR and SHAM selectively inhibited tyrosinase isoforms separated by native electrophoresis. At low concentrations, 4HR was a selective inhibitor of tyrosinase and could differentiate between tyrosinase and laccase activities.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><doi>10.1016/0031-9422(93)85503-J</doi><tpages>4</tpages></addata></record> |
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| ispartof | Phytochemistry (Oxford), 1993-05, Vol.33 (2), p.281-284 |
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| subjects | 4-hexylresorcinol Agaricaceae Agaricus bisporus Agronomy. Soil science and plant productions Biological and medical sciences differentiation Enzymes Fundamental and applied biological sciences. Psychology fungi Hyphomycetes inhibition laccase Metabolism Plant physiology and development Pyricularia oryzae tyrosinase |
| title | Differentiation of tyrosinase and laccase using 4-hexyl-resorcinol, a tyrosinase inhibitor |
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