Detection of Renibacterium salmoninarum in naturally infected Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum) using an enzyme-linked immunosorbent assay
A double-antibody enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Renibacterium salmoninarum. The ELISA assay was rapid and sensitive, with a lower limit of detection of 0.3 μg R. salmoninarum antigen per ml. Under field conditions with naturally infected fish, the ELISA...
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| Veröffentlicht in: | Aquaculture 1993-10, Vol.116 (2), p.99-110 |
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| creator | Olea, Itziar Bruno, David W. Hastings, Trevor S. |
| description | A double-antibody enzyme-linked immunosorbent assay (ELISA) was developed for the detection of
Renibacterium salmoninarum. The ELISA assay was rapid and sensitive, with a lower limit of detection of 0.3
μg
R. salmoninarum antigen per ml. Under field conditions with naturally infected fish, the ELISA proved more sensitive than bacteriological methods, and therefore has the potential for the rapid screening of fish from wild or farmed populations for evidence of BKD infection. However, in some cases fish with resolving BKD lesions (i.e. previously infected) were not detected by this method. |
| doi_str_mv | 10.1016/0044-8486(93)90001-F |
| format | Article |
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μg
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Renibacterium salmoninarum. The ELISA assay was rapid and sensitive, with a lower limit of detection of 0.3
μg
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Psychology</subject><subject>Marine</subject><subject>ONCORHYNCHUS</subject><subject>Oncorhynchus mykiss</subject><subject>Pisciculture</subject><subject>RENIBACTERIUM SALMONINARUM</subject><subject>SALMO SALAR</subject><subject>TEST ELISA</subject><subject>Vertebrate aquaculture</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp9kU1v1DAQhiMEEkvhDyAOFkKolTbFThw7viBVhQWkFZX4EEdr1pm0bhO72A4o_DR-HQ676oEDp9Fonne-3qJ4xugpo0y8opTzsuWtOFb1iaKUsnJzr1ixVtZlI6rqfrG6Qx4Wj2K8zowQDVsVv99gQpOsd8T35BM6uwOTMNhpJBGG0TvrIOTEOuIgTQGGYc5Jn0XYkbM0gEvWHNg1-bzEJYNAtqdrAq4jAazb-Z8kBT-lNblwxoer2ZmrKZJxvrExkuNvMOxgGk_IFK27zDKC7tc8YjlYd5MH2XGcnI8-7NAlAjHC_Lh40MMQ8ckhHhVfN2-_nL8vtxfvPpyfbUvDWZVKzhVyUbVK8hpVW7dKGCmxapAz1nCkIBVUsgPVSypqpahqc5ka2fRtD119VLzc970N_vuEMenRRoNDvhz9FDUTUsi6qTL4_B_w2k_B5d10RblQlEmaIb6HTPAxBuz1bbAjhFkzqhc39WKVXqzSqtZ_3dSbLHtx6A3RwNAHcMbGO20teVupOmNP91gPXsNlyMjHrarzXc0y-vW-iPlbPywGHY1FZ7CzIdupO2__v8Qf_lW9xA</recordid><startdate>19931001</startdate><enddate>19931001</enddate><creator>Olea, Itziar</creator><creator>Bruno, David W.</creator><creator>Hastings, Trevor S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>H97</scope></search><sort><creationdate>19931001</creationdate><title>Detection of Renibacterium salmoninarum in naturally infected Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum) using an enzyme-linked immunosorbent assay</title><author>Olea, Itziar ; Bruno, David W. ; Hastings, Trevor S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-449e46289743e983896c77e25e41154e0a79a27da9f7063990987e20c75f8fad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Brackish</topic><topic>ELISA</topic><topic>Fish</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. 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Renibacterium salmoninarum. The ELISA assay was rapid and sensitive, with a lower limit of detection of 0.3
μg
R. salmoninarum antigen per ml. Under field conditions with naturally infected fish, the ELISA proved more sensitive than bacteriological methods, and therefore has the potential for the rapid screening of fish from wild or farmed populations for evidence of BKD infection. However, in some cases fish with resolving BKD lesions (i.e. previously infected) were not detected by this method.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/0044-8486(93)90001-F</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0044-8486 |
| ispartof | Aquaculture, 1993-10, Vol.116 (2), p.99-110 |
| issn | 0044-8486 1873-5622 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings |
| subjects | Animal aquaculture Animal productions ANTIGENE ANTIGENOS ANTIGENS Bacteriology Biological and medical sciences Brackish ELISA Fish Freshwater Fundamental and applied biological sciences. Psychology Marine ONCORHYNCHUS Oncorhynchus mykiss Pisciculture RENIBACTERIUM SALMONINARUM SALMO SALAR TEST ELISA Vertebrate aquaculture |
| title | Detection of Renibacterium salmoninarum in naturally infected Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum) using an enzyme-linked immunosorbent assay |
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