Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms
•Tetracycline did not affect the removal of carbon and nitrogen.•The antibiotic affected the bacterial composition of the biofilms.•The tetracycline removal was poor (28%).•Biodegradation was probably the main removal mechanism of the antibiotic.•The occurrence of tet(S) was influenced by the presen...
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| Veröffentlicht in: | Chemosphere (Oxford) 2014-12, Vol.117 (1), p.295-302 |
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| creator | Matos, Maria Pereira, Maria A. Parpot, Pier Brito, António G. Nogueira, Regina |
| description | •Tetracycline did not affect the removal of carbon and nitrogen.•The antibiotic affected the bacterial composition of the biofilms.•The tetracycline removal was poor (28%).•Biodegradation was probably the main removal mechanism of the antibiotic.•The occurrence of tet(S) was influenced by the presence of tetracycline.
The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic. |
| doi_str_mv | 10.1016/j.chemosphere.2014.06.094 |
| format | Article |
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The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>EISSN: 0045-6535</identifier><identifier>DOI: 10.1016/j.chemosphere.2014.06.094</identifier><identifier>PMID: 25127228</identifier><identifier>CODEN: CMSHAF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adsorption ; Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Applied sciences ; Bacteria ; Bacterial community ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biodegradation ; Biofilms ; Biofilms - drug effects ; Biological and medical sciences ; Biological treatment of waters ; Biotechnology ; Communities ; Environment and pollution ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General purification processes ; Genes ; Industrial applications and implications. Economical aspects ; Microbiota - drug effects ; Microbiota - physiology ; Molecular Sequence Data ; Nitrification - drug effects ; Nitrifying biofilms ; Pollution ; Polymers - metabolism ; Resistance genes ; Science & Technology ; Sequence Analysis, DNA ; Tetracycline - metabolism ; Tetracycline - pharmacology ; Tetracycline removal ; Tetracycline Resistance ; Tetracyclines ; Waste water ; Wastewaters ; Water treatment and pollution</subject><ispartof>Chemosphere (Oxford), 2014-12, Vol.117 (1), p.295-302</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-78e30f1f90c4f8065a6cb38f90627fb5b9c853b421b8603b2df4852b02766f1e3</citedby><cites>FETCH-LOGICAL-c549t-78e30f1f90c4f8065a6cb38f90627fb5b9c853b421b8603b2df4852b02766f1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0045653514008935$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=29022367$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25127228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matos, Maria</creatorcontrib><creatorcontrib>Pereira, Maria A.</creatorcontrib><creatorcontrib>Parpot, Pier</creatorcontrib><creatorcontrib>Brito, António G.</creatorcontrib><creatorcontrib>Nogueira, Regina</creatorcontrib><title>Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>•Tetracycline did not affect the removal of carbon and nitrogen.•The antibiotic affected the bacterial composition of the biofilms.•The tetracycline removal was poor (28%).•Biodegradation was probably the main removal mechanism of the antibiotic.•The occurrence of tet(S) was influenced by the presence of tetracycline.
The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.</description><subject>Adsorption</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Applied sciences</subject><subject>Bacteria</subject><subject>Bacterial community</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biodegradation</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biotechnology</subject><subject>Communities</subject><subject>Environment and pollution</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General purification processes</subject><subject>Genes</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Microbiota - drug effects</subject><subject>Microbiota - physiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrification - drug effects</subject><subject>Nitrifying biofilms</subject><subject>Pollution</subject><subject>Polymers - metabolism</subject><subject>Resistance genes</subject><subject>Science & Technology</subject><subject>Sequence Analysis, DNA</subject><subject>Tetracycline - metabolism</subject><subject>Tetracycline - pharmacology</subject><subject>Tetracycline removal</subject><subject>Tetracycline Resistance</subject><subject>Tetracyclines</subject><subject>Waste water</subject><subject>Wastewaters</subject><subject>Water treatment and pollution</subject><issn>0045-6535</issn><issn>1879-1298</issn><issn>0045-6535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkuPFCEURonROO3oXzDlwsRNlRcoKFiajo9JJnGjawIU2HTqJVCd9L-XstvHsldww7mXLxwQeoOhwYD5-2NjD26c03Jw0TUEcNsAb0C2T9AOi07WmEjxFO0AWlZzRtkdepHSEaA0M_kc3RGGSUeI2KH-YfLD6ibrqtlX2eWo7dkOYSr1VOWDq8Zg42yCHio7j-M6hXzedsucQg6F0VNfaZvDaTsoMwoQgz-H6UdlwuzDMKaX6JnXQ3Kvrus9-v7p47f9l_rx6-eH_YfH2rJW5roTjoLHXoJtvQDONLeGilJz0nnDjLSCUdMSbAQHakjvW8GIAdJx7rGj9-jdZe4S55-rS1mNIVk3DHpy85oU5h2nnFDCbkA5QAcd725AiWSCSUILKi9oebKUovNqiWHU8awwqE2dOqr_1KlNnQKuirrS-_p6zWpG1__t_OOqAG-vgE5WDz7qyYb0j5NACP2dt7pw0Wq9qOhOIWVdcgpCFMW83XLuL4grNk7BRZVs2D5BH6KzWfVzuCHxL4ggyCA</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Matos, Maria</creator><creator>Pereira, Maria A.</creator><creator>Parpot, Pier</creator><creator>Brito, António G.</creator><creator>Nogueira, Regina</creator><general>Elsevier Ltd</general><general>Pergamon Press Ltd</general><general>Elsevier</general><scope>RCLKO</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope><scope>7QH</scope><scope>7T7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20141201</creationdate><title>Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms</title><author>Matos, Maria ; Pereira, Maria A. ; Parpot, Pier ; Brito, António G. ; Nogueira, Regina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-78e30f1f90c4f8065a6cb38f90627fb5b9c853b421b8603b2df4852b02766f1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adsorption</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Applied sciences</topic><topic>Bacteria</topic><topic>Bacterial community</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biodegradation</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of waters</topic><topic>Biotechnology</topic><topic>Communities</topic><topic>Environment and pollution</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General purification processes</topic><topic>Genes</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Microbiota - drug effects</topic><topic>Microbiota - physiology</topic><topic>Molecular Sequence Data</topic><topic>Nitrification - drug effects</topic><topic>Nitrifying biofilms</topic><topic>Pollution</topic><topic>Polymers - metabolism</topic><topic>Resistance genes</topic><topic>Science & Technology</topic><topic>Sequence Analysis, DNA</topic><topic>Tetracycline - metabolism</topic><topic>Tetracycline - pharmacology</topic><topic>Tetracycline removal</topic><topic>Tetracycline Resistance</topic><topic>Tetracyclines</topic><topic>Waste water</topic><topic>Wastewaters</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matos, Maria</creatorcontrib><creatorcontrib>Pereira, Maria A.</creatorcontrib><creatorcontrib>Parpot, Pier</creatorcontrib><creatorcontrib>Brito, António G.</creatorcontrib><creatorcontrib>Nogueira, Regina</creatorcontrib><collection>RCAAP open access repository</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Aqualine</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matos, Maria</au><au>Pereira, Maria A.</au><au>Parpot, Pier</au><au>Brito, António G.</au><au>Nogueira, Regina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>117</volume><issue>1</issue><spage>295</spage><epage>302</epage><pages>295-302</pages><issn>0045-6535</issn><eissn>1879-1298</eissn><eissn>0045-6535</eissn><coden>CMSHAF</coden><abstract>•Tetracycline did not affect the removal of carbon and nitrogen.•The antibiotic affected the bacterial composition of the biofilms.•The tetracycline removal was poor (28%).•Biodegradation was probably the main removal mechanism of the antibiotic.•The occurrence of tet(S) was influenced by the presence of tetracycline.
The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>25127228</pmid><doi>10.1016/j.chemosphere.2014.06.094</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adsorption Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Antibiotics Applied sciences Bacteria Bacterial community Bacterial Proteins - genetics Bacterial Proteins - metabolism Biodegradation Biofilms Biofilms - drug effects Biological and medical sciences Biological treatment of waters Biotechnology Communities Environment and pollution Exact sciences and technology Fundamental and applied biological sciences. Psychology General purification processes Genes Industrial applications and implications. Economical aspects Microbiota - drug effects Microbiota - physiology Molecular Sequence Data Nitrification - drug effects Nitrifying biofilms Pollution Polymers - metabolism Resistance genes Science & Technology Sequence Analysis, DNA Tetracycline - metabolism Tetracycline - pharmacology Tetracycline removal Tetracycline Resistance Tetracyclines Waste water Wastewaters Water treatment and pollution |
| title | Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
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