Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs
► This is the first construction of recombinant SPV expressing HA1 of H3N2 SIV. ► The recombinant rSPV-H3 can express HA1 of H3N2 SIV in vitro. ► rSPV-H3 can induce specific humoral and cell-mediated immune responses in animals. ► Administration of rSPV-H3 provides a complete protection against H3N2...
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| Veröffentlicht in: | Virus research 2012-08, Vol.167 (2), p.188-195 |
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| creator | Xu, Jiarong Huang, Dongyan Liu, Shichao Lin, Huixing Zhu, Haodan Liu, Bao Lu, Chengping |
| description | ► This is the first construction of recombinant SPV expressing HA1 of H3N2 SIV. ► The recombinant rSPV-H3 can express HA1 of H3N2 SIV in vitro. ► rSPV-H3 can induce specific humoral and cell-mediated immune responses in animals. ► Administration of rSPV-H3 provides a complete protection against H3N2 SIV in pigs.
Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Even though immunization with recombinant vaccinia poxviruses expressing protective antigens as a vaccination strategy has been widely used for many infectious diseases, development of recombinant swinepox virus (rSPV) vector for this purpose has been less successful. Here, we report the construction of a recombinant swinepox virus (rSPV) expressing hemagglutinin (HA1) of H3N2 SIV (rSPV-H3). Immune responses and protection efficacy of the vaccination vector were assessed in both mouse and pig models. Prime and boost inoculations of rSPV-H3 yielded neutralization antibody against SIV and elicited potent H3N2 SIV-specific INF-γ response from T-lymphocytes. Complete protection of pigs against H3N2 SIV challenge was achieved. No pigs showed severe systemic and local reactions and no SIV was found shed from the pigs vaccinated with rSPV-H3 after challenge. The data suggest that the SPV-based recombinant vector expressing HA1 of H3N2 SIV might serve as a promising SIV vaccine for protection against SIV infection. |
| doi_str_mv | 10.1016/j.virusres.2012.04.014 |
| format | Article |
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Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Even though immunization with recombinant vaccinia poxviruses expressing protective antigens as a vaccination strategy has been widely used for many infectious diseases, development of recombinant swinepox virus (rSPV) vector for this purpose has been less successful. Here, we report the construction of a recombinant swinepox virus (rSPV) expressing hemagglutinin (HA1) of H3N2 SIV (rSPV-H3). Immune responses and protection efficacy of the vaccination vector were assessed in both mouse and pig models. Prime and boost inoculations of rSPV-H3 yielded neutralization antibody against SIV and elicited potent H3N2 SIV-specific INF-γ response from T-lymphocytes. Complete protection of pigs against H3N2 SIV challenge was achieved. No pigs showed severe systemic and local reactions and no SIV was found shed from the pigs vaccinated with rSPV-H3 after challenge. The data suggest that the SPV-based recombinant vector expressing HA1 of H3N2 SIV might serve as a promising SIV vaccine for protection against SIV infection.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2012.04.014</identifier><identifier>PMID: 22584406</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animal trial ; Animals ; antibodies ; Antibodies, Neutralizing - blood ; Antibodies, Viral - blood ; antigens ; Drug Carriers - administration & dosage ; Female ; Hemagglutinin ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; hemagglutinins ; human health ; immune response ; Immunogenicity ; infectious diseases ; Influenza A virus ; Influenza A Virus, H3N2 Subtype - genetics ; Influenza A Virus, H3N2 Subtype - immunology ; Influenza Vaccines - administration & dosage ; Influenza Vaccines - genetics ; Influenza Vaccines - immunology ; Influenza virus ; interferon-gamma ; Interferon-gamma - secretion ; Mice ; Mice, Inbred BALB C ; neutralization ; Orthomyxoviridae Infections - immunology ; Orthomyxoviridae Infections - pathology ; Orthomyxoviridae Infections - prevention & control ; Orthomyxoviridae Infections - virology ; pathogens ; Protection efficacy ; Recombinant swinepox virus ; Simian immunodeficiency virus ; Suipoxvirus - genetics ; Swine ; Swine H3N2 influenza virus ; Swine influenza virus ; Swinepox virus ; T-lymphocytes ; T-Lymphocytes - immunology ; vaccination ; Vaccination - methods ; vaccines ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - genetics ; Vaccines, Synthetic - immunology ; Virus Shedding</subject><ispartof>Virus research, 2012-08, Vol.167 (2), p.188-195</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-77d04237e8aa41fa5e8f4a31b527cda6e380dfdac6e2191952c4f0078ffd6ed33</citedby><cites>FETCH-LOGICAL-c425t-77d04237e8aa41fa5e8f4a31b527cda6e380dfdac6e2191952c4f0078ffd6ed33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168170212001542$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22584406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Jiarong</creatorcontrib><creatorcontrib>Huang, Dongyan</creatorcontrib><creatorcontrib>Liu, Shichao</creatorcontrib><creatorcontrib>Lin, Huixing</creatorcontrib><creatorcontrib>Zhu, Haodan</creatorcontrib><creatorcontrib>Liu, Bao</creatorcontrib><creatorcontrib>Lu, Chengping</creatorcontrib><title>Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>► This is the first construction of recombinant SPV expressing HA1 of H3N2 SIV. ► The recombinant rSPV-H3 can express HA1 of H3N2 SIV in vitro. ► rSPV-H3 can induce specific humoral and cell-mediated immune responses in animals. ► Administration of rSPV-H3 provides a complete protection against H3N2 SIV in pigs.
Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Even though immunization with recombinant vaccinia poxviruses expressing protective antigens as a vaccination strategy has been widely used for many infectious diseases, development of recombinant swinepox virus (rSPV) vector for this purpose has been less successful. Here, we report the construction of a recombinant swinepox virus (rSPV) expressing hemagglutinin (HA1) of H3N2 SIV (rSPV-H3). Immune responses and protection efficacy of the vaccination vector were assessed in both mouse and pig models. Prime and boost inoculations of rSPV-H3 yielded neutralization antibody against SIV and elicited potent H3N2 SIV-specific INF-γ response from T-lymphocytes. Complete protection of pigs against H3N2 SIV challenge was achieved. No pigs showed severe systemic and local reactions and no SIV was found shed from the pigs vaccinated with rSPV-H3 after challenge. The data suggest that the SPV-based recombinant vector expressing HA1 of H3N2 SIV might serve as a promising SIV vaccine for protection against SIV infection.</description><subject>Animal trial</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Neutralizing - blood</subject><subject>Antibodies, Viral - blood</subject><subject>antigens</subject><subject>Drug Carriers - administration & dosage</subject><subject>Female</subject><subject>Hemagglutinin</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - genetics</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - immunology</subject><subject>hemagglutinins</subject><subject>human health</subject><subject>immune response</subject><subject>Immunogenicity</subject><subject>infectious diseases</subject><subject>Influenza A virus</subject><subject>Influenza A Virus, H3N2 Subtype - genetics</subject><subject>Influenza A Virus, H3N2 Subtype - immunology</subject><subject>Influenza Vaccines - administration & dosage</subject><subject>Influenza Vaccines - genetics</subject><subject>Influenza Vaccines - immunology</subject><subject>Influenza virus</subject><subject>interferon-gamma</subject><subject>Interferon-gamma - secretion</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>neutralization</subject><subject>Orthomyxoviridae Infections - immunology</subject><subject>Orthomyxoviridae Infections - pathology</subject><subject>Orthomyxoviridae Infections - prevention & control</subject><subject>Orthomyxoviridae Infections - virology</subject><subject>pathogens</subject><subject>Protection efficacy</subject><subject>Recombinant swinepox virus</subject><subject>Simian immunodeficiency virus</subject><subject>Suipoxvirus - genetics</subject><subject>Swine</subject><subject>Swine H3N2 influenza virus</subject><subject>Swine influenza virus</subject><subject>Swinepox virus</subject><subject>T-lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>vaccination</subject><subject>Vaccination - methods</subject><subject>vaccines</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - genetics</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Virus Shedding</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EosPAKxQv2STYjmNndlQVMJUqWEDXlse-Ht3RxA5x0h9egZfGJVO2XXlz7nc_30PIOWc1Z1x9PNS3OM55hFwLxkXNZM24fEFWvNOi0nIjXpJVAbuKaybOyJucD4wx1Wj1mpwJ0XZSMrUif676fo5AS9CQYoZMbfR0GNMEbsIUKYSAzroHmgK1BXOp32G0caL5DiMM6Z7-a0LhfighGeOebi84tXuLMZ8oum2-CYoxHGeIv-1pAiPt0cGyEff5LXkV7DHDu9O7JjdfPv-83FbX379eXV5cV06Kdqq09kyKRkNnreTBttAFaRu-a4V23ipoOuaDt06B4Bu-aYWTgTHdheAV-KZZkw9LbvnmrxnyZHrMDo5HGyHN2XClVdNuxEY9jzKhVKd46bMmakHdmHIRE8wwYm_HhwKZR2fmYJ6cmUdnhklTnJXB89OOedeD_z_2JKkA7xcg2GTsfsRsbn6UBFWEcq2VLsSnhYBytluE0WSHEB14LMYm4xM-1-IvZjy3OA</recordid><startdate>20120801</startdate><enddate>20120801</enddate><creator>Xu, Jiarong</creator><creator>Huang, Dongyan</creator><creator>Liu, Shichao</creator><creator>Lin, Huixing</creator><creator>Zhu, Haodan</creator><creator>Liu, Bao</creator><creator>Lu, Chengping</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20120801</creationdate><title>Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs</title><author>Xu, Jiarong ; Huang, Dongyan ; Liu, Shichao ; Lin, Huixing ; Zhu, Haodan ; Liu, Bao ; Lu, Chengping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-77d04237e8aa41fa5e8f4a31b527cda6e380dfdac6e2191952c4f0078ffd6ed33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal trial</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Neutralizing - blood</topic><topic>Antibodies, Viral - blood</topic><topic>antigens</topic><topic>Drug Carriers - administration & dosage</topic><topic>Female</topic><topic>Hemagglutinin</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - genetics</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - immunology</topic><topic>hemagglutinins</topic><topic>human health</topic><topic>immune response</topic><topic>Immunogenicity</topic><topic>infectious diseases</topic><topic>Influenza A virus</topic><topic>Influenza A Virus, H3N2 Subtype - genetics</topic><topic>Influenza A Virus, H3N2 Subtype - immunology</topic><topic>Influenza Vaccines - administration & dosage</topic><topic>Influenza Vaccines - genetics</topic><topic>Influenza Vaccines - immunology</topic><topic>Influenza virus</topic><topic>interferon-gamma</topic><topic>Interferon-gamma - secretion</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>neutralization</topic><topic>Orthomyxoviridae Infections - immunology</topic><topic>Orthomyxoviridae Infections - pathology</topic><topic>Orthomyxoviridae Infections - prevention & control</topic><topic>Orthomyxoviridae Infections - virology</topic><topic>pathogens</topic><topic>Protection efficacy</topic><topic>Recombinant swinepox virus</topic><topic>Simian immunodeficiency virus</topic><topic>Suipoxvirus - genetics</topic><topic>Swine</topic><topic>Swine H3N2 influenza virus</topic><topic>Swine influenza virus</topic><topic>Swinepox virus</topic><topic>T-lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>vaccination</topic><topic>Vaccination - methods</topic><topic>vaccines</topic><topic>Vaccines, Synthetic - administration & dosage</topic><topic>Vaccines, Synthetic - genetics</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Virus Shedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Jiarong</creatorcontrib><creatorcontrib>Huang, Dongyan</creatorcontrib><creatorcontrib>Liu, Shichao</creatorcontrib><creatorcontrib>Lin, Huixing</creatorcontrib><creatorcontrib>Zhu, Haodan</creatorcontrib><creatorcontrib>Liu, Bao</creatorcontrib><creatorcontrib>Lu, Chengping</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Jiarong</au><au>Huang, Dongyan</au><au>Liu, Shichao</au><au>Lin, Huixing</au><au>Zhu, Haodan</au><au>Liu, Bao</au><au>Lu, Chengping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2012-08-01</date><risdate>2012</risdate><volume>167</volume><issue>2</issue><spage>188</spage><epage>195</epage><pages>188-195</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>► This is the first construction of recombinant SPV expressing HA1 of H3N2 SIV. ► The recombinant rSPV-H3 can express HA1 of H3N2 SIV in vitro. ► rSPV-H3 can induce specific humoral and cell-mediated immune responses in animals. ► Administration of rSPV-H3 provides a complete protection against H3N2 SIV in pigs.
Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Even though immunization with recombinant vaccinia poxviruses expressing protective antigens as a vaccination strategy has been widely used for many infectious diseases, development of recombinant swinepox virus (rSPV) vector for this purpose has been less successful. Here, we report the construction of a recombinant swinepox virus (rSPV) expressing hemagglutinin (HA1) of H3N2 SIV (rSPV-H3). Immune responses and protection efficacy of the vaccination vector were assessed in both mouse and pig models. Prime and boost inoculations of rSPV-H3 yielded neutralization antibody against SIV and elicited potent H3N2 SIV-specific INF-γ response from T-lymphocytes. Complete protection of pigs against H3N2 SIV challenge was achieved. No pigs showed severe systemic and local reactions and no SIV was found shed from the pigs vaccinated with rSPV-H3 after challenge. The data suggest that the SPV-based recombinant vector expressing HA1 of H3N2 SIV might serve as a promising SIV vaccine for protection against SIV infection.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22584406</pmid><doi>10.1016/j.virusres.2012.04.014</doi><tpages>8</tpages></addata></record> |
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| subjects | Animal trial Animals antibodies Antibodies, Neutralizing - blood Antibodies, Viral - blood antigens Drug Carriers - administration & dosage Female Hemagglutinin Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology hemagglutinins human health immune response Immunogenicity infectious diseases Influenza A virus Influenza A Virus, H3N2 Subtype - genetics Influenza A Virus, H3N2 Subtype - immunology Influenza Vaccines - administration & dosage Influenza Vaccines - genetics Influenza Vaccines - immunology Influenza virus interferon-gamma Interferon-gamma - secretion Mice Mice, Inbred BALB C neutralization Orthomyxoviridae Infections - immunology Orthomyxoviridae Infections - pathology Orthomyxoviridae Infections - prevention & control Orthomyxoviridae Infections - virology pathogens Protection efficacy Recombinant swinepox virus Simian immunodeficiency virus Suipoxvirus - genetics Swine Swine H3N2 influenza virus Swine influenza virus Swinepox virus T-lymphocytes T-Lymphocytes - immunology vaccination Vaccination - methods vaccines Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - genetics Vaccines, Synthetic - immunology Virus Shedding |
| title | Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs |
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