Predator cues magnify effects of the pesticide endosulfan in water bugs in a multi-species test in outdoor containers

•Proof of predator cues magnifying pesticide stress is limited to the laboratory.•We for the first time document this synergism in semi-natural outdoor conditions.•Only with predator cues endosulfan reduced body mass in four water bug species.•This synergism may be underestimated under optimal food levels in the laboratory.•This highlights the need for ecological realism when testing effects of pollutants. Pesticides have become major stressors in many aquatic communities. Laboratory studies suggest their impact may be further magnified in the presence of cues from predators. Despite their importance for ecological risk assessment, synergisms between pesticides and predator cues have not been confirmed under semi-natural outdoor conditions. We evaluated how the presence of predator cues and the presence of a non-corixid community affect the pesticide sensitivity of five water bug (Corixidae) species in an outdoor, multi-species container experiment. The experiment employed a full factorial design with two pesticide treatments, two predator cue treatments and two (non-corixid) community treatments (absence versus presence of Cloeon dipterum mayfly larvae, Ischnura elegans damselfly larvae and Physa acuta snails). The pesticide treatment negatively affected survival in Cymatia coleoptrata, and to a lesser extent, Sigara lateralis, but not in the other three Corixidae species (Hesperocorixa linnaei, Sigara iactans and Sigara striata). The addition of pesticides did not significantly affect body mass in the latter four species, unless combined with predator cues. To our knowledge this is the first report of this synergism under semi-natural, outdoor conditions. Neither lethal nor sublethal pesticide effects in the Corixidae depended on the community context, yet the presence of the non-corixid community when combined with predator cues reduced survival and body mass. Our results suggest that the here documented synergism between pesticides and predator cues may occur in nature.