Cardioprotective effects of a proanthocyanidin-rich fraction from Croton celtidifolius Baill: Focus on atherosclerosis
► Proanthocyanidins from Croton celtidifolius prevent human LDL oxidation. ► PRF protect endothelial cells from cytotoxic effects of oxidized LDL. ► PRF improve endothelial function in LDLr−/− mice. Proanthocyanidins are the most abundant polyphenols in human diets. Epidemiological studies have poin...
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| Veröffentlicht in: | Food and chemical toxicology 2012-10, Vol.50 (10), p.3769-3775 |
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| creator | Hort, Mariana Appel Straliotto, Marcos Raniel Duz, Mariana Silveira Netto, Paula Moro Souza, Christiane Borges Schulz, Tamirys Horst, Heros Pizzolatti, Moacir Geraldo de Bem, Andreza Fabro Ribeiro-do-Valle, Rosa Maria |
| description | ► Proanthocyanidins from Croton celtidifolius prevent human LDL oxidation. ► PRF protect endothelial cells from cytotoxic effects of oxidized LDL. ► PRF improve endothelial function in LDLr−/− mice.
Proanthocyanidins are the most abundant polyphenols in human diets. Epidemiological studies have pointed to proanthocyanidins as promising molecules that could prevent the development of several coronary syndromes by inhibiting the atherogenic process. The present study was designed to investigate the antiatherogenic effects of a proanthocyanidin-rich fraction (PRF) obtained from Croton celtidifolius Baill (Euphorbiaceae) barks. In isolated human LDL, PRF caused a concentration-dependent inhibition of Cu2+-induced oxidative modifications, evidenced by the increasing of the lag phase of lipid peroxidation and decreasing in the oxidation rate (Vmax), moreover, the protein moieties from LDL were protected against Cu2+-induced oxidation. In human umbilical vein endothelial cells (HUVECs), PRF reduced the ROS production stimulated by oxidized LDL. Herein, we demonstrate that oral treatment with PRF improved endothelium-dependent vasorelaxation in hypercholesterolemic LDL receptor knockout mice (LDLr−/−), however, the fraction did not modify plasma lipids and atherosclerotic lesion size in this experimental model. Finally, our results showed for the first time that PRF prevent isolated LDL oxidation, decrease oxidative stress in endothelial cells and improve endothelial function in mice. |
| doi_str_mv | 10.1016/j.fct.2012.07.050 |
| format | Article |
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Proanthocyanidins are the most abundant polyphenols in human diets. Epidemiological studies have pointed to proanthocyanidins as promising molecules that could prevent the development of several coronary syndromes by inhibiting the atherogenic process. The present study was designed to investigate the antiatherogenic effects of a proanthocyanidin-rich fraction (PRF) obtained from Croton celtidifolius Baill (Euphorbiaceae) barks. In isolated human LDL, PRF caused a concentration-dependent inhibition of Cu2+-induced oxidative modifications, evidenced by the increasing of the lag phase of lipid peroxidation and decreasing in the oxidation rate (Vmax), moreover, the protein moieties from LDL were protected against Cu2+-induced oxidation. In human umbilical vein endothelial cells (HUVECs), PRF reduced the ROS production stimulated by oxidized LDL. Herein, we demonstrate that oral treatment with PRF improved endothelium-dependent vasorelaxation in hypercholesterolemic LDL receptor knockout mice (LDLr−/−), however, the fraction did not modify plasma lipids and atherosclerotic lesion size in this experimental model. Finally, our results showed for the first time that PRF prevent isolated LDL oxidation, decrease oxidative stress in endothelial cells and improve endothelial function in mice.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2012.07.050</identifier><identifier>PMID: 22884763</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Atherosclerosis ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Cells, Cultured ; Cholesterol, LDL - chemistry ; Copper ; Coronary Artery Disease - prevention & control ; Croton ; Croton - chemistry ; Croton celtidifolius ; Endothelial cells ; Endothelial Cells - drug effects ; Euphorbiaceae ; Medical sciences ; Mice ; Mice, Knockout ; Oxidation-Reduction ; Oxidative Stress ; Plant Bark - chemistry ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Proanthocyanidins ; Proanthocyanidins - chemistry ; Proanthocyanidins - pharmacology ; Receptors, LDL - genetics ; Receptors, LDL - metabolism ; Toxicology ; Vasodilation - drug effects</subject><ispartof>Food and chemical toxicology, 2012-10, Vol.50 (10), p.3769-3775</ispartof><rights>2012 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-6f6b88e5d5a6e1a2ce421b3f520eadee40ec10af5f987f4c3edc3b202a44fe7d3</citedby><cites>FETCH-LOGICAL-c482t-6f6b88e5d5a6e1a2ce421b3f520eadee40ec10af5f987f4c3edc3b202a44fe7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2012.07.050$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26418845$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22884763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hort, Mariana Appel</creatorcontrib><creatorcontrib>Straliotto, Marcos Raniel</creatorcontrib><creatorcontrib>Duz, Mariana Silveira</creatorcontrib><creatorcontrib>Netto, Paula Moro</creatorcontrib><creatorcontrib>Souza, Christiane Borges</creatorcontrib><creatorcontrib>Schulz, Tamirys</creatorcontrib><creatorcontrib>Horst, Heros</creatorcontrib><creatorcontrib>Pizzolatti, Moacir Geraldo</creatorcontrib><creatorcontrib>de Bem, Andreza Fabro</creatorcontrib><creatorcontrib>Ribeiro-do-Valle, Rosa Maria</creatorcontrib><title>Cardioprotective effects of a proanthocyanidin-rich fraction from Croton celtidifolius Baill: Focus on atherosclerosis</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>► Proanthocyanidins from Croton celtidifolius prevent human LDL oxidation. ► PRF protect endothelial cells from cytotoxic effects of oxidized LDL. ► PRF improve endothelial function in LDLr−/− mice.
Proanthocyanidins are the most abundant polyphenols in human diets. Epidemiological studies have pointed to proanthocyanidins as promising molecules that could prevent the development of several coronary syndromes by inhibiting the atherogenic process. The present study was designed to investigate the antiatherogenic effects of a proanthocyanidin-rich fraction (PRF) obtained from Croton celtidifolius Baill (Euphorbiaceae) barks. In isolated human LDL, PRF caused a concentration-dependent inhibition of Cu2+-induced oxidative modifications, evidenced by the increasing of the lag phase of lipid peroxidation and decreasing in the oxidation rate (Vmax), moreover, the protein moieties from LDL were protected against Cu2+-induced oxidation. In human umbilical vein endothelial cells (HUVECs), PRF reduced the ROS production stimulated by oxidized LDL. Herein, we demonstrate that oral treatment with PRF improved endothelium-dependent vasorelaxation in hypercholesterolemic LDL receptor knockout mice (LDLr−/−), however, the fraction did not modify plasma lipids and atherosclerotic lesion size in this experimental model. Finally, our results showed for the first time that PRF prevent isolated LDL oxidation, decrease oxidative stress in endothelial cells and improve endothelial function in mice.</description><subject>Animals</subject><subject>Atherosclerosis</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Cells, Cultured</subject><subject>Cholesterol, LDL - chemistry</subject><subject>Copper</subject><subject>Coronary Artery Disease - prevention & control</subject><subject>Croton</subject><subject>Croton - chemistry</subject><subject>Croton celtidifolius</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - drug effects</subject><subject>Euphorbiaceae</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress</subject><subject>Plant Bark - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Proanthocyanidins</subject><subject>Proanthocyanidins - chemistry</subject><subject>Proanthocyanidins - pharmacology</subject><subject>Receptors, LDL - genetics</subject><subject>Receptors, LDL - metabolism</subject><subject>Toxicology</subject><subject>Vasodilation - drug effects</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi1URNPCD-CC9lKJyy5jrz-25QRRC0iVuMDZcuyx4mizbu1NpP57JkqgN3qxZ-xnRjPvy9h7Dh0Hrj9tuujnTgAXHZgOFLxiCz6YvtW94mdsAcIMrb7m6pxd1LoBAMONfsPOhRgGaXS_YPulKyHlh5Jn9HPaY4MxUlSbHBvX0Lub5nX2T25KIU1tSX7dxOKIzRMFedssqZZij-NMSMxj2tXmq0vjeNPcZU8J_bp5jSVXPx7OVN-y19GNFd-d7kv2--721_J7e__z24_ll_vWy0HMrY56NQyognIauRMepeCrPioB6AKiBPQcXFTxejBR-h6D71cChJMyogn9Jft47EuLPO6wznabKk06ugnzrlquSQUFJNfLKBiSTEg4oPyIelqmFoz2oaStK08E2YMzdmPJGXtwxoKx5AzVfDi13622GP5V_LWCgKsT4Kp3I2k8-VSfOS05kYq4z0cOSbd9wmKrTzh5DKmQcTbk9J8x_gCkja5K</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Hort, Mariana Appel</creator><creator>Straliotto, Marcos Raniel</creator><creator>Duz, Mariana Silveira</creator><creator>Netto, Paula Moro</creator><creator>Souza, Christiane Borges</creator><creator>Schulz, Tamirys</creator><creator>Horst, Heros</creator><creator>Pizzolatti, Moacir Geraldo</creator><creator>de Bem, Andreza Fabro</creator><creator>Ribeiro-do-Valle, Rosa Maria</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20121001</creationdate><title>Cardioprotective effects of a proanthocyanidin-rich fraction from Croton celtidifolius Baill: Focus on atherosclerosis</title><author>Hort, Mariana Appel ; Straliotto, Marcos Raniel ; Duz, Mariana Silveira ; Netto, Paula Moro ; Souza, Christiane Borges ; Schulz, Tamirys ; Horst, Heros ; Pizzolatti, Moacir Geraldo ; de Bem, Andreza Fabro ; Ribeiro-do-Valle, Rosa Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-6f6b88e5d5a6e1a2ce421b3f520eadee40ec10af5f987f4c3edc3b202a44fe7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Atherosclerosis</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Cells, Cultured</topic><topic>Cholesterol, LDL - chemistry</topic><topic>Copper</topic><topic>Coronary Artery Disease - prevention & control</topic><topic>Croton</topic><topic>Croton - chemistry</topic><topic>Croton celtidifolius</topic><topic>Endothelial cells</topic><topic>Endothelial Cells - drug effects</topic><topic>Euphorbiaceae</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress</topic><topic>Plant Bark - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Proanthocyanidins</topic><topic>Proanthocyanidins - chemistry</topic><topic>Proanthocyanidins - pharmacology</topic><topic>Receptors, LDL - genetics</topic><topic>Receptors, LDL - metabolism</topic><topic>Toxicology</topic><topic>Vasodilation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hort, Mariana Appel</creatorcontrib><creatorcontrib>Straliotto, Marcos Raniel</creatorcontrib><creatorcontrib>Duz, Mariana Silveira</creatorcontrib><creatorcontrib>Netto, Paula Moro</creatorcontrib><creatorcontrib>Souza, Christiane Borges</creatorcontrib><creatorcontrib>Schulz, Tamirys</creatorcontrib><creatorcontrib>Horst, Heros</creatorcontrib><creatorcontrib>Pizzolatti, Moacir Geraldo</creatorcontrib><creatorcontrib>de Bem, Andreza Fabro</creatorcontrib><creatorcontrib>Ribeiro-do-Valle, Rosa Maria</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hort, Mariana Appel</au><au>Straliotto, Marcos Raniel</au><au>Duz, Mariana Silveira</au><au>Netto, Paula Moro</au><au>Souza, Christiane Borges</au><au>Schulz, Tamirys</au><au>Horst, Heros</au><au>Pizzolatti, Moacir Geraldo</au><au>de Bem, Andreza Fabro</au><au>Ribeiro-do-Valle, Rosa Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cardioprotective effects of a proanthocyanidin-rich fraction from Croton celtidifolius Baill: Focus on atherosclerosis</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>50</volume><issue>10</issue><spage>3769</spage><epage>3775</epage><pages>3769-3775</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>► Proanthocyanidins from Croton celtidifolius prevent human LDL oxidation. ► PRF protect endothelial cells from cytotoxic effects of oxidized LDL. ► PRF improve endothelial function in LDLr−/− mice.
Proanthocyanidins are the most abundant polyphenols in human diets. Epidemiological studies have pointed to proanthocyanidins as promising molecules that could prevent the development of several coronary syndromes by inhibiting the atherogenic process. The present study was designed to investigate the antiatherogenic effects of a proanthocyanidin-rich fraction (PRF) obtained from Croton celtidifolius Baill (Euphorbiaceae) barks. In isolated human LDL, PRF caused a concentration-dependent inhibition of Cu2+-induced oxidative modifications, evidenced by the increasing of the lag phase of lipid peroxidation and decreasing in the oxidation rate (Vmax), moreover, the protein moieties from LDL were protected against Cu2+-induced oxidation. In human umbilical vein endothelial cells (HUVECs), PRF reduced the ROS production stimulated by oxidized LDL. Herein, we demonstrate that oral treatment with PRF improved endothelium-dependent vasorelaxation in hypercholesterolemic LDL receptor knockout mice (LDLr−/−), however, the fraction did not modify plasma lipids and atherosclerotic lesion size in this experimental model. Finally, our results showed for the first time that PRF prevent isolated LDL oxidation, decrease oxidative stress in endothelial cells and improve endothelial function in mice.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>22884763</pmid><doi>10.1016/j.fct.2012.07.050</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Atherosclerosis Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Cells, Cultured Cholesterol, LDL - chemistry Copper Coronary Artery Disease - prevention & control Croton Croton - chemistry Croton celtidifolius Endothelial cells Endothelial Cells - drug effects Euphorbiaceae Medical sciences Mice Mice, Knockout Oxidation-Reduction Oxidative Stress Plant Bark - chemistry Plant Extracts - chemistry Plant Extracts - pharmacology Proanthocyanidins Proanthocyanidins - chemistry Proanthocyanidins - pharmacology Receptors, LDL - genetics Receptors, LDL - metabolism Toxicology Vasodilation - drug effects |
| title | Cardioprotective effects of a proanthocyanidin-rich fraction from Croton celtidifolius Baill: Focus on atherosclerosis |
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