Waterborne exposure of zebrafish embryos to micromole concentrations of ioxynil and diethylstilbestrol disrupts thyrocyte development
•Micromolar concentrations of waterborne IOX and DES disrupt thyrocyte embryonic development in zebrafish.•IOX and DES do not seem to directly interact with components of thyroid hormone genomic and non-genomic cellular pathways.•IOX but most notably DES disrupt thyrocyte development indirectly via...
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| Veröffentlicht in: | Aquatic toxicology 2013-09, Vol.140-141, p.279-287 |
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| creator | Campinho, M.A. Power, D.M. |
| description | •Micromolar concentrations of waterborne IOX and DES disrupt thyrocyte embryonic development in zebrafish.•IOX and DES do not seem to directly interact with components of thyroid hormone genomic and non-genomic cellular pathways.•IOX but most notably DES disrupt thyrocyte development indirectly via altering heart development.
The herbicide ioxynil (IOX) and synthetic estrogen diethylstilbestrol (DES) are common aquatic contaminants with an endocrine disrupting action. In juvenile teleost fish IOX and DES disrupt the hypothalamic–pituitary–thyroid (HPT) axis. To assess how IOX and DES influence the developing HPT axis prior to establishment of central regulation of thyroid hormones, zebrafish embryos were exposed to low concentrations of the chemicals in water. IOX and DES (1 and 0.1μM) exposure failed to modify hypothalamic development but had a negative effect on thyrocyte development. Specifically, IOX and DES caused a significant (p |
| doi_str_mv | 10.1016/j.aquatox.2013.06.014 |
| format | Article |
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The herbicide ioxynil (IOX) and synthetic estrogen diethylstilbestrol (DES) are common aquatic contaminants with an endocrine disrupting action. In juvenile teleost fish IOX and DES disrupt the hypothalamic–pituitary–thyroid (HPT) axis. To assess how IOX and DES influence the developing HPT axis prior to establishment of central regulation of thyroid hormones, zebrafish embryos were exposed to low concentrations of the chemicals in water. IOX and DES (1 and 0.1μM) exposure failed to modify hypothalamic development but had a negative effect on thyrocyte development. Specifically, IOX and DES caused a significant (p<0.05) reduction in the size of the thyroid anlagen by decreasing the mRNA expression field of both nk2.1a and thyroglobulin (Tg) genes. Inhibition of thyroid gland development by IOX and DES (0.1μM) was strongly associated with altered heart morphology. To test if the effect of IOX and DES on the thyroid was a consequence of altered cardiac development a morpholino (MO) against zebrafish cardiac troponin I (zcTnI) was microinjected. The zcTnI morphants had modified heart function, a small thyroid anlagen and a reduction in the mRNA expression of nk2.1a and Tg genes similar to that of zebrafish exposed to IOX (1 and 0.1μM) and DES (0.1μM). Collectively the data indicate that IOX and DES alter thyroid development in zebrafish and chemicals that alter heart development and function can have an indirect endocrine disrupting action on the thyroid in teleosts.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2013.06.014</identifier><identifier>PMID: 23851054</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Danio rerio ; Development ; Diethylstilbestrol ; Diethylstilbestrol - toxicity ; Embryo, Nonmammalian - drug effects ; Endocrine disruption ; fish ; gene expression ; Gene Expression Regulation - drug effects ; genes ; heart ; Heart - drug effects ; Hypothalamus - drug effects ; Iodobenzenes - toxicity ; Ioxynil ; messenger RNA ; Nitriles - toxicity ; NK Cell Lectin-Like Receptor Subfamily A - genetics ; pollutants ; Teleostei ; Thyrocytes ; Thyroglobulin - genetics ; thyroid gland ; Thyroid Gland - cytology ; Thyroid Gland - drug effects ; thyroid hormones ; Water Pollutants, Chemical - toxicity ; Zebrafish ; Zebrafish - physiology</subject><ispartof>Aquatic toxicology, 2013-09, Vol.140-141, p.279-287</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-3db9e7c71771e236f2051ef86712810eadf8b4428d7eb3d210a960e589b03ce3</citedby><cites>FETCH-LOGICAL-c422t-3db9e7c71771e236f2051ef86712810eadf8b4428d7eb3d210a960e589b03ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166445X13001574$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23851054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campinho, M.A.</creatorcontrib><creatorcontrib>Power, D.M.</creatorcontrib><title>Waterborne exposure of zebrafish embryos to micromole concentrations of ioxynil and diethylstilbestrol disrupts thyrocyte development</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>•Micromolar concentrations of waterborne IOX and DES disrupt thyrocyte embryonic development in zebrafish.•IOX and DES do not seem to directly interact with components of thyroid hormone genomic and non-genomic cellular pathways.•IOX but most notably DES disrupt thyrocyte development indirectly via altering heart development.
The herbicide ioxynil (IOX) and synthetic estrogen diethylstilbestrol (DES) are common aquatic contaminants with an endocrine disrupting action. In juvenile teleost fish IOX and DES disrupt the hypothalamic–pituitary–thyroid (HPT) axis. To assess how IOX and DES influence the developing HPT axis prior to establishment of central regulation of thyroid hormones, zebrafish embryos were exposed to low concentrations of the chemicals in water. IOX and DES (1 and 0.1μM) exposure failed to modify hypothalamic development but had a negative effect on thyrocyte development. Specifically, IOX and DES caused a significant (p<0.05) reduction in the size of the thyroid anlagen by decreasing the mRNA expression field of both nk2.1a and thyroglobulin (Tg) genes. Inhibition of thyroid gland development by IOX and DES (0.1μM) was strongly associated with altered heart morphology. To test if the effect of IOX and DES on the thyroid was a consequence of altered cardiac development a morpholino (MO) against zebrafish cardiac troponin I (zcTnI) was microinjected. The zcTnI morphants had modified heart function, a small thyroid anlagen and a reduction in the mRNA expression of nk2.1a and Tg genes similar to that of zebrafish exposed to IOX (1 and 0.1μM) and DES (0.1μM). Collectively the data indicate that IOX and DES alter thyroid development in zebrafish and chemicals that alter heart development and function can have an indirect endocrine disrupting action on the thyroid in teleosts.</description><subject>Animals</subject><subject>Danio rerio</subject><subject>Development</subject><subject>Diethylstilbestrol</subject><subject>Diethylstilbestrol - toxicity</subject><subject>Embryo, Nonmammalian - drug effects</subject><subject>Endocrine disruption</subject><subject>fish</subject><subject>gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>genes</subject><subject>heart</subject><subject>Heart - drug effects</subject><subject>Hypothalamus - drug effects</subject><subject>Iodobenzenes - toxicity</subject><subject>Ioxynil</subject><subject>messenger RNA</subject><subject>Nitriles - toxicity</subject><subject>NK Cell Lectin-Like Receptor Subfamily A - genetics</subject><subject>pollutants</subject><subject>Teleostei</subject><subject>Thyrocytes</subject><subject>Thyroglobulin - genetics</subject><subject>thyroid gland</subject><subject>Thyroid Gland - cytology</subject><subject>Thyroid Gland - drug effects</subject><subject>thyroid hormones</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Zebrafish</subject><subject>Zebrafish - physiology</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAUhC1ERbeFnwD4yCXBdhwnOSFUFYpUqQeK4GY59gvrlROntlNtuPd_49UuXOuLJeub8XszCL2lpKSEio-7Uj0sKvl9yQitSiJKQvkLtKFt0xW0pvwl2mROFJzXv87RRYw7kg_j3St0zqq2pqTmG_T0UyUIvQ8TYNjPPi4BsB_wH-iDGmzcYhj7sPqIk8ej1cGP3gHWftIwpaCS9VM8CKzfr5N1WE0GGwtpu7qYrOshpuBdfophmVO22a7B6zUBNvAIzs9j9nmNzgblIrw53Zfo_sv1_dVNcXv39dvV59tCc8ZSUZm-g0Y3tGkosEoMjNQUhlY0lLWUgDJD23POWtNAXxlGieoEgbrtelJpqC7Rh6PtHPzDkieTo40anFMT-CVKKhpR8a4hIqP1Ec0bxxhgkHOwowqrpEQeCpA7eSpAHgqQRMhcQNa9O32x9COY_6p_iWfg_REYlJfqd7BR_vieHTjJppyKOhOfjgTkJB4tBBm1hZy3sQF0ksbbZ4b4C5D9p8U</recordid><startdate>20130915</startdate><enddate>20130915</enddate><creator>Campinho, M.A.</creator><creator>Power, D.M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope></search><sort><creationdate>20130915</creationdate><title>Waterborne exposure of zebrafish embryos to micromole concentrations of ioxynil and diethylstilbestrol disrupts thyrocyte development</title><author>Campinho, M.A. ; Power, D.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-3db9e7c71771e236f2051ef86712810eadf8b4428d7eb3d210a960e589b03ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Danio rerio</topic><topic>Development</topic><topic>Diethylstilbestrol</topic><topic>Diethylstilbestrol - toxicity</topic><topic>Embryo, Nonmammalian - drug effects</topic><topic>Endocrine disruption</topic><topic>fish</topic><topic>gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>genes</topic><topic>heart</topic><topic>Heart - drug effects</topic><topic>Hypothalamus - drug effects</topic><topic>Iodobenzenes - toxicity</topic><topic>Ioxynil</topic><topic>messenger RNA</topic><topic>Nitriles - toxicity</topic><topic>NK Cell Lectin-Like Receptor Subfamily A - genetics</topic><topic>pollutants</topic><topic>Teleostei</topic><topic>Thyrocytes</topic><topic>Thyroglobulin - genetics</topic><topic>thyroid gland</topic><topic>Thyroid Gland - cytology</topic><topic>Thyroid Gland - drug effects</topic><topic>thyroid hormones</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>Zebrafish</topic><topic>Zebrafish - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campinho, M.A.</creatorcontrib><creatorcontrib>Power, D.M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campinho, M.A.</au><au>Power, D.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Waterborne exposure of zebrafish embryos to micromole concentrations of ioxynil and diethylstilbestrol disrupts thyrocyte development</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2013-09-15</date><risdate>2013</risdate><volume>140-141</volume><spage>279</spage><epage>287</epage><pages>279-287</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><abstract>•Micromolar concentrations of waterborne IOX and DES disrupt thyrocyte embryonic development in zebrafish.•IOX and DES do not seem to directly interact with components of thyroid hormone genomic and non-genomic cellular pathways.•IOX but most notably DES disrupt thyrocyte development indirectly via altering heart development.
The herbicide ioxynil (IOX) and synthetic estrogen diethylstilbestrol (DES) are common aquatic contaminants with an endocrine disrupting action. In juvenile teleost fish IOX and DES disrupt the hypothalamic–pituitary–thyroid (HPT) axis. To assess how IOX and DES influence the developing HPT axis prior to establishment of central regulation of thyroid hormones, zebrafish embryos were exposed to low concentrations of the chemicals in water. IOX and DES (1 and 0.1μM) exposure failed to modify hypothalamic development but had a negative effect on thyrocyte development. Specifically, IOX and DES caused a significant (p<0.05) reduction in the size of the thyroid anlagen by decreasing the mRNA expression field of both nk2.1a and thyroglobulin (Tg) genes. Inhibition of thyroid gland development by IOX and DES (0.1μM) was strongly associated with altered heart morphology. To test if the effect of IOX and DES on the thyroid was a consequence of altered cardiac development a morpholino (MO) against zebrafish cardiac troponin I (zcTnI) was microinjected. The zcTnI morphants had modified heart function, a small thyroid anlagen and a reduction in the mRNA expression of nk2.1a and Tg genes similar to that of zebrafish exposed to IOX (1 and 0.1μM) and DES (0.1μM). Collectively the data indicate that IOX and DES alter thyroid development in zebrafish and chemicals that alter heart development and function can have an indirect endocrine disrupting action on the thyroid in teleosts.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23851054</pmid><doi>10.1016/j.aquatox.2013.06.014</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Danio rerio Development Diethylstilbestrol Diethylstilbestrol - toxicity Embryo, Nonmammalian - drug effects Endocrine disruption fish gene expression Gene Expression Regulation - drug effects genes heart Heart - drug effects Hypothalamus - drug effects Iodobenzenes - toxicity Ioxynil messenger RNA Nitriles - toxicity NK Cell Lectin-Like Receptor Subfamily A - genetics pollutants Teleostei Thyrocytes Thyroglobulin - genetics thyroid gland Thyroid Gland - cytology Thyroid Gland - drug effects thyroid hormones Water Pollutants, Chemical - toxicity Zebrafish Zebrafish - physiology |
| title | Waterborne exposure of zebrafish embryos to micromole concentrations of ioxynil and diethylstilbestrol disrupts thyrocyte development |
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