Role of prostacyclin in acetylcholine release from myenteric plexus of guinea-pig ileum
The roles of metabolites of arachidonic acid in spontaneous and agonist-induced acetylcholine release from a longitudinal muscle preparation with myenteric plexus of guinea-pig ileum were studied. Indomethacin significantly decreased both spontaneous acetylcholine release and its release induced by...
Gespeichert in:
| Veröffentlicht in: | European journal of pharmacology 1993-03, Vol.233 (2), p.237-242 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 242 |
|---|---|
| container_issue | 2 |
| container_start_page | 237 |
| container_title | European journal of pharmacology |
| container_volume | 233 |
| creator | Fukunaga, Yuko Mine, Yukiko Yoshikawa, Shiyu Takeuchi, Tadayoshi Hata, Fumiaki Yagasaki, Osamu |
| description | The roles of metabolites of arachidonic acid in spontaneous and agonist-induced acetylcholine release from a longitudinal muscle preparation with myenteric plexus of guinea-pig ileum were studied. Indomethacin significantly decreased both spontaneous acetylcholine release and its release induced by nicotine and substance P. We had found that prostaglandin E
2 (PGE
2) partly reversed this inhibition. We now found that a stable prostacyclin analog, OP-41483 at 100 nM, completely reversed the inhibition of acetylcholine release by indomethacin. On the other hand, PGD
2, PGF
2α and ONO-11113, a thromboxane A
2 analog, did not have any significant effect on the inhibition by indomethacin. OP-41483 had no effect on acetylcholine release induced by nicotine or substance P in the absence of indomethacin. To confirm the modulatory role of endogenous prostaglandins on acetylcholine release, we also studied the release of 6-keto-PGF
1α, a metabolite of prostacyclin, and PGE
2 from longitudinal muscle preparations. The preparations released appreciable amounts of 6-keto-PGF
1α continuously during the experiments. Indomethacin inhibited release, while nicotine did not affect it so significantly. Our results suggest that endogenous prostacyclin modulates acetylcholine release from cholinergic nerve terminals in the myenteric plexus of guinea-pig ileum. |
| doi_str_mv | 10.1016/0014-2999(93)90055-M |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16763094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>001429999390055M</els_id><sourcerecordid>16763094</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-5eb8991c6ab90afadbb2da170008a4121e3ac76f099cd316f255f22f3c9fd4c33</originalsourceid><addsrcrecordid>eNp9kF1r2zAUhkVZybK0_2ADX4yyXbiV_CH73BRKabtCQqFs9FLIx0edhmxnkl2af1-lCbksCIQ4z3t49TD2VfBzwYW84FwUaQYAPyD_CZyXZbo6YnNRV5DySmSf2PyAfGZfQvjHIwRZOWOzupBVLWHOnh4HR8lgkrUfwqhxg872STwaadw4_DvENyWeHOlAifFDl3Qb6kfyFpO1o9cpbOPPU8R0urbPiXU0dSfs2GgX6HR_L9if25vf17_S5cPd_fXVMsVCVGNaUlMDCJS6Aa6Nbpsma7WoYtNaFyITlGuspOEA2OZCmqwsTZaZHMG0Beb5gp3t9sb-_ycKo-psQHJO9zRMQQlZyZxDEcFiB2L8aPBk1NrbTvuNElxtfaqtLLWVpSBX7z7VKsa-7fdPTUftIbQXGOff93MdUDvjdY82HLBCljUHHrHLHUbRxYslrwJa6pFa6wlH1Q724x5vZyaSng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16763094</pqid></control><display><type>article</type><title>Role of prostacyclin in acetylcholine release from myenteric plexus of guinea-pig ileum</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Fukunaga, Yuko ; Mine, Yukiko ; Yoshikawa, Shiyu ; Takeuchi, Tadayoshi ; Hata, Fumiaki ; Yagasaki, Osamu</creator><creatorcontrib>Fukunaga, Yuko ; Mine, Yukiko ; Yoshikawa, Shiyu ; Takeuchi, Tadayoshi ; Hata, Fumiaki ; Yagasaki, Osamu</creatorcontrib><description>The roles of metabolites of arachidonic acid in spontaneous and agonist-induced acetylcholine release from a longitudinal muscle preparation with myenteric plexus of guinea-pig ileum were studied. Indomethacin significantly decreased both spontaneous acetylcholine release and its release induced by nicotine and substance P. We had found that prostaglandin E
2 (PGE
2) partly reversed this inhibition. We now found that a stable prostacyclin analog, OP-41483 at 100 nM, completely reversed the inhibition of acetylcholine release by indomethacin. On the other hand, PGD
2, PGF
2α and ONO-11113, a thromboxane A
2 analog, did not have any significant effect on the inhibition by indomethacin. OP-41483 had no effect on acetylcholine release induced by nicotine or substance P in the absence of indomethacin. To confirm the modulatory role of endogenous prostaglandins on acetylcholine release, we also studied the release of 6-keto-PGF
1α, a metabolite of prostacyclin, and PGE
2 from longitudinal muscle preparations. The preparations released appreciable amounts of 6-keto-PGF
1α continuously during the experiments. Indomethacin inhibited release, while nicotine did not affect it so significantly. Our results suggest that endogenous prostacyclin modulates acetylcholine release from cholinergic nerve terminals in the myenteric plexus of guinea-pig ileum.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(93)90055-M</identifier><identifier>PMID: 8467869</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetylcholine - metabolism ; Acetylcholine release ; Animals ; Biological and medical sciences ; Drug Interactions ; Epoprostenol - analogs & derivatives ; Epoprostenol - pharmacology ; Epoprostenol - physiology ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Ileum - innervation ; Ileum - metabolism ; Indomethacin - pharmacology ; Intestine. Mesentery ; Male ; Myenteric plexus ; Myenteric Plexus - drug effects ; Myenteric Plexus - metabolism ; Nicotine - pharmacology ; OP-41483 (prostacyclin analog) ; Platelet Aggregation Inhibitors - pharmacology ; Prostaglandin release ; Prostaglandins - metabolism ; Prostaglandins - pharmacology ; Vertebrates: digestive system</subject><ispartof>European journal of pharmacology, 1993-03, Vol.233 (2), p.237-242</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-5eb8991c6ab90afadbb2da170008a4121e3ac76f099cd316f255f22f3c9fd4c33</citedby><cites>FETCH-LOGICAL-c417t-5eb8991c6ab90afadbb2da170008a4121e3ac76f099cd316f255f22f3c9fd4c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(93)90055-M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4658090$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8467869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukunaga, Yuko</creatorcontrib><creatorcontrib>Mine, Yukiko</creatorcontrib><creatorcontrib>Yoshikawa, Shiyu</creatorcontrib><creatorcontrib>Takeuchi, Tadayoshi</creatorcontrib><creatorcontrib>Hata, Fumiaki</creatorcontrib><creatorcontrib>Yagasaki, Osamu</creatorcontrib><title>Role of prostacyclin in acetylcholine release from myenteric plexus of guinea-pig ileum</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The roles of metabolites of arachidonic acid in spontaneous and agonist-induced acetylcholine release from a longitudinal muscle preparation with myenteric plexus of guinea-pig ileum were studied. Indomethacin significantly decreased both spontaneous acetylcholine release and its release induced by nicotine and substance P. We had found that prostaglandin E
2 (PGE
2) partly reversed this inhibition. We now found that a stable prostacyclin analog, OP-41483 at 100 nM, completely reversed the inhibition of acetylcholine release by indomethacin. On the other hand, PGD
2, PGF
2α and ONO-11113, a thromboxane A
2 analog, did not have any significant effect on the inhibition by indomethacin. OP-41483 had no effect on acetylcholine release induced by nicotine or substance P in the absence of indomethacin. To confirm the modulatory role of endogenous prostaglandins on acetylcholine release, we also studied the release of 6-keto-PGF
1α, a metabolite of prostacyclin, and PGE
2 from longitudinal muscle preparations. The preparations released appreciable amounts of 6-keto-PGF
1α continuously during the experiments. Indomethacin inhibited release, while nicotine did not affect it so significantly. Our results suggest that endogenous prostacyclin modulates acetylcholine release from cholinergic nerve terminals in the myenteric plexus of guinea-pig ileum.</description><subject>Acetylcholine - metabolism</subject><subject>Acetylcholine release</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Drug Interactions</subject><subject>Epoprostenol - analogs & derivatives</subject><subject>Epoprostenol - pharmacology</subject><subject>Epoprostenol - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Ileum - innervation</subject><subject>Ileum - metabolism</subject><subject>Indomethacin - pharmacology</subject><subject>Intestine. Mesentery</subject><subject>Male</subject><subject>Myenteric plexus</subject><subject>Myenteric Plexus - drug effects</subject><subject>Myenteric Plexus - metabolism</subject><subject>Nicotine - pharmacology</subject><subject>OP-41483 (prostacyclin analog)</subject><subject>Platelet Aggregation Inhibitors - pharmacology</subject><subject>Prostaglandin release</subject><subject>Prostaglandins - metabolism</subject><subject>Prostaglandins - pharmacology</subject><subject>Vertebrates: digestive system</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1r2zAUhkVZybK0_2ADX4yyXbiV_CH73BRKabtCQqFs9FLIx0edhmxnkl2af1-lCbksCIQ4z3t49TD2VfBzwYW84FwUaQYAPyD_CZyXZbo6YnNRV5DySmSf2PyAfGZfQvjHIwRZOWOzupBVLWHOnh4HR8lgkrUfwqhxg872STwaadw4_DvENyWeHOlAifFDl3Qb6kfyFpO1o9cpbOPPU8R0urbPiXU0dSfs2GgX6HR_L9if25vf17_S5cPd_fXVMsVCVGNaUlMDCJS6Aa6Nbpsma7WoYtNaFyITlGuspOEA2OZCmqwsTZaZHMG0Beb5gp3t9sb-_ycKo-psQHJO9zRMQQlZyZxDEcFiB2L8aPBk1NrbTvuNElxtfaqtLLWVpSBX7z7VKsa-7fdPTUftIbQXGOff93MdUDvjdY82HLBCljUHHrHLHUbRxYslrwJa6pFa6wlH1Q724x5vZyaSng</recordid><startdate>19930323</startdate><enddate>19930323</enddate><creator>Fukunaga, Yuko</creator><creator>Mine, Yukiko</creator><creator>Yoshikawa, Shiyu</creator><creator>Takeuchi, Tadayoshi</creator><creator>Hata, Fumiaki</creator><creator>Yagasaki, Osamu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19930323</creationdate><title>Role of prostacyclin in acetylcholine release from myenteric plexus of guinea-pig ileum</title><author>Fukunaga, Yuko ; Mine, Yukiko ; Yoshikawa, Shiyu ; Takeuchi, Tadayoshi ; Hata, Fumiaki ; Yagasaki, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-5eb8991c6ab90afadbb2da170008a4121e3ac76f099cd316f255f22f3c9fd4c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetylcholine - metabolism</topic><topic>Acetylcholine release</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Drug Interactions</topic><topic>Epoprostenol - analogs & derivatives</topic><topic>Epoprostenol - pharmacology</topic><topic>Epoprostenol - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Ileum - innervation</topic><topic>Ileum - metabolism</topic><topic>Indomethacin - pharmacology</topic><topic>Intestine. Mesentery</topic><topic>Male</topic><topic>Myenteric plexus</topic><topic>Myenteric Plexus - drug effects</topic><topic>Myenteric Plexus - metabolism</topic><topic>Nicotine - pharmacology</topic><topic>OP-41483 (prostacyclin analog)</topic><topic>Platelet Aggregation Inhibitors - pharmacology</topic><topic>Prostaglandin release</topic><topic>Prostaglandins - metabolism</topic><topic>Prostaglandins - pharmacology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukunaga, Yuko</creatorcontrib><creatorcontrib>Mine, Yukiko</creatorcontrib><creatorcontrib>Yoshikawa, Shiyu</creatorcontrib><creatorcontrib>Takeuchi, Tadayoshi</creatorcontrib><creatorcontrib>Hata, Fumiaki</creatorcontrib><creatorcontrib>Yagasaki, Osamu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukunaga, Yuko</au><au>Mine, Yukiko</au><au>Yoshikawa, Shiyu</au><au>Takeuchi, Tadayoshi</au><au>Hata, Fumiaki</au><au>Yagasaki, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of prostacyclin in acetylcholine release from myenteric plexus of guinea-pig ileum</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1993-03-23</date><risdate>1993</risdate><volume>233</volume><issue>2</issue><spage>237</spage><epage>242</epage><pages>237-242</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>The roles of metabolites of arachidonic acid in spontaneous and agonist-induced acetylcholine release from a longitudinal muscle preparation with myenteric plexus of guinea-pig ileum were studied. Indomethacin significantly decreased both spontaneous acetylcholine release and its release induced by nicotine and substance P. We had found that prostaglandin E
2 (PGE
2) partly reversed this inhibition. We now found that a stable prostacyclin analog, OP-41483 at 100 nM, completely reversed the inhibition of acetylcholine release by indomethacin. On the other hand, PGD
2, PGF
2α and ONO-11113, a thromboxane A
2 analog, did not have any significant effect on the inhibition by indomethacin. OP-41483 had no effect on acetylcholine release induced by nicotine or substance P in the absence of indomethacin. To confirm the modulatory role of endogenous prostaglandins on acetylcholine release, we also studied the release of 6-keto-PGF
1α, a metabolite of prostacyclin, and PGE
2 from longitudinal muscle preparations. The preparations released appreciable amounts of 6-keto-PGF
1α continuously during the experiments. Indomethacin inhibited release, while nicotine did not affect it so significantly. Our results suggest that endogenous prostacyclin modulates acetylcholine release from cholinergic nerve terminals in the myenteric plexus of guinea-pig ileum.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8467869</pmid><doi>10.1016/0014-2999(93)90055-M</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2999 |
| ispartof | European journal of pharmacology, 1993-03, Vol.233 (2), p.237-242 |
| issn | 0014-2999 1879-0712 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16763094 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetylcholine - metabolism Acetylcholine release Animals Biological and medical sciences Drug Interactions Epoprostenol - analogs & derivatives Epoprostenol - pharmacology Epoprostenol - physiology Fundamental and applied biological sciences. Psychology Guinea Pigs Ileum - innervation Ileum - metabolism Indomethacin - pharmacology Intestine. Mesentery Male Myenteric plexus Myenteric Plexus - drug effects Myenteric Plexus - metabolism Nicotine - pharmacology OP-41483 (prostacyclin analog) Platelet Aggregation Inhibitors - pharmacology Prostaglandin release Prostaglandins - metabolism Prostaglandins - pharmacology Vertebrates: digestive system |
| title | Role of prostacyclin in acetylcholine release from myenteric plexus of guinea-pig ileum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T05%3A21%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20prostacyclin%20in%20acetylcholine%20release%20from%20myenteric%20plexus%20of%20guinea-pig%20ileum&rft.jtitle=European%20journal%20of%20pharmacology&rft.au=Fukunaga,%20Yuko&rft.date=1993-03-23&rft.volume=233&rft.issue=2&rft.spage=237&rft.epage=242&rft.pages=237-242&rft.issn=0014-2999&rft.eissn=1879-0712&rft.coden=EJPHAZ&rft_id=info:doi/10.1016/0014-2999(93)90055-M&rft_dat=%3Cproquest_cross%3E16763094%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16763094&rft_id=info:pmid/8467869&rft_els_id=001429999390055M&rfr_iscdi=true |