Effects of age and zinc supplementation on transport properties in the jejunum of piglets
Zinc is effective in the prevention and treatment of post‐weaning diarrhoea and in promoting piglet growth. Its effects on the absorption of nutrients and the secretory capacity of the intestinal epithelium are controversial. We investigated the effects of age, dietary pharmacological zinc supplemen...
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| Veröffentlicht in: | Journal of animal physiology and animal nutrition 2015-06, Vol.99 (3), p.542-552 |
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| creator | Gefeller, E. M Martens, H Aschenbach, J. R Klingspor, S Twardziok, S Wrede, P Pieper, R Lodemann, U |
| description | Zinc is effective in the prevention and treatment of post‐weaning diarrhoea and in promoting piglet growth. Its effects on the absorption of nutrients and the secretory capacity of the intestinal epithelium are controversial. We investigated the effects of age, dietary pharmacological zinc supplementation and acute zinc exposure in vitro on small‐intestinal transport properties of weaned piglets. We further examined whether the effect of zinc on secretory responses depended on the pathway by which chloride secretion is activated. A total of 96 piglets were weaned at 26 days of age and allocated to diets containing three different levels of zinc oxide (50, 150 and 2500 ppm). At the age of 32, 39, 46 and 53 days, piglets were killed, and isolated epithelia from the mid‐jejunum were used for intestinal transport studies in conventional Ussing chambers, with 23 μm ZnSO₄being added to the serosal side for testing acute effects. Absorptive transport was stimulated by mucosal addition of d‐glucose or l‐glutamine. Secretion was activated by serosal addition of prostaglandin E₂, carbachol or by mucosal application of Escherichia coli heat‐stable enterotoxin (Stₚ). Jejunal transport properties showed significant age‐dependent alterations (p |
| doi_str_mv | 10.1111/jpn.12232 |
| format | Article |
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M ; Martens, H ; Aschenbach, J. R ; Klingspor, S ; Twardziok, S ; Wrede, P ; Pieper, R ; Lodemann, U</creator><creatorcontrib>Gefeller, E. M ; Martens, H ; Aschenbach, J. R ; Klingspor, S ; Twardziok, S ; Wrede, P ; Pieper, R ; Lodemann, U</creatorcontrib><description>Zinc is effective in the prevention and treatment of post‐weaning diarrhoea and in promoting piglet growth. Its effects on the absorption of nutrients and the secretory capacity of the intestinal epithelium are controversial. We investigated the effects of age, dietary pharmacological zinc supplementation and acute zinc exposure in vitro on small‐intestinal transport properties of weaned piglets. We further examined whether the effect of zinc on secretory responses depended on the pathway by which chloride secretion is activated. A total of 96 piglets were weaned at 26 days of age and allocated to diets containing three different levels of zinc oxide (50, 150 and 2500 ppm). At the age of 32, 39, 46 and 53 days, piglets were killed, and isolated epithelia from the mid‐jejunum were used for intestinal transport studies in conventional Ussing chambers, with 23 μm ZnSO₄being added to the serosal side for testing acute effects. Absorptive transport was stimulated by mucosal addition of d‐glucose or l‐glutamine. Secretion was activated by serosal addition of prostaglandin E₂, carbachol or by mucosal application of Escherichia coli heat‐stable enterotoxin (Stₚ). Jejunal transport properties showed significant age‐dependent alterations (p < 0.03). Both absorptive and secretory responses were highest in the youngest piglets (32 d). The dietary zinc supplementation had no significant influence on jejunal absorptive and secretory responses. However, the pre‐treatment of epithelia with ZnSO₄in vitro led to a small but significant decrease in both absorptive and secretory capacities (p < 0.05), with an exception for carbachol (p = 0.07). The results showed that, in piglets, chronic supplementation with zinc did not sustainably influence the jejunal transport properties in the post‐weaning phase. Because transport properties are influenced by the addition of zinc in vitro, we suggest that possible epithelial effects of zinc depend on the acute presence of this ion.</description><identifier>ISSN: 0931-2439</identifier><identifier>EISSN: 1439-0396</identifier><identifier>DOI: 10.1111/jpn.12232</identifier><identifier>PMID: 25039419</identifier><language>eng</language><publisher>Germany: Blackwell Science</publisher><subject>absorption ; acute effects ; age ; Aging ; Animal Feed - analysis ; Animal Nutritional Physiological Phenomena ; Animals ; Biological Transport ; carbachol ; chloride secretion ; diarrhea ; Diet - veterinary ; dietary mineral supplements ; Dietary Supplements ; Dose-Response Relationship, Drug ; enterotoxins ; epithelium ; Escherichia coli ; glucose ; glutamine ; heat stability ; intestinal mucosa ; ion transport ; jejunum ; Jejunum - drug effects ; Jejunum - metabolism ; nutrients ; pig ; piglets ; prostaglandins ; secretion ; small intestine ; Swine - physiology ; Ussing chamber ; zinc ; zinc oxide ; Zinc Oxide - administration & dosage ; Zinc Oxide - pharmacology</subject><ispartof>Journal of animal physiology and animal nutrition, 2015-06, Vol.99 (3), p.542-552</ispartof><rights>Journal of Animal Physiology and Animal Nutrition © 2014 Blackwell Verlag GmbH</rights><rights>Journal of Animal Physiology and Animal Nutrition © 2014 Blackwell Verlag GmbH.</rights><rights>2015 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4152-284e5e0ac453f3863a7d3adf67b2b741cb922e39d132b1b2a98adcc1e76fbe4d3</citedby><cites>FETCH-LOGICAL-c4152-284e5e0ac453f3863a7d3adf67b2b741cb922e39d132b1b2a98adcc1e76fbe4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjpn.12232$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjpn.12232$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25039419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gefeller, E. M</creatorcontrib><creatorcontrib>Martens, H</creatorcontrib><creatorcontrib>Aschenbach, J. R</creatorcontrib><creatorcontrib>Klingspor, S</creatorcontrib><creatorcontrib>Twardziok, S</creatorcontrib><creatorcontrib>Wrede, P</creatorcontrib><creatorcontrib>Pieper, R</creatorcontrib><creatorcontrib>Lodemann, U</creatorcontrib><title>Effects of age and zinc supplementation on transport properties in the jejunum of piglets</title><title>Journal of animal physiology and animal nutrition</title><addtitle>J Anim Physiol Anim Nutr</addtitle><description>Zinc is effective in the prevention and treatment of post‐weaning diarrhoea and in promoting piglet growth. Its effects on the absorption of nutrients and the secretory capacity of the intestinal epithelium are controversial. We investigated the effects of age, dietary pharmacological zinc supplementation and acute zinc exposure in vitro on small‐intestinal transport properties of weaned piglets. We further examined whether the effect of zinc on secretory responses depended on the pathway by which chloride secretion is activated. A total of 96 piglets were weaned at 26 days of age and allocated to diets containing three different levels of zinc oxide (50, 150 and 2500 ppm). At the age of 32, 39, 46 and 53 days, piglets were killed, and isolated epithelia from the mid‐jejunum were used for intestinal transport studies in conventional Ussing chambers, with 23 μm ZnSO₄being added to the serosal side for testing acute effects. Absorptive transport was stimulated by mucosal addition of d‐glucose or l‐glutamine. Secretion was activated by serosal addition of prostaglandin E₂, carbachol or by mucosal application of Escherichia coli heat‐stable enterotoxin (Stₚ). Jejunal transport properties showed significant age‐dependent alterations (p < 0.03). Both absorptive and secretory responses were highest in the youngest piglets (32 d). The dietary zinc supplementation had no significant influence on jejunal absorptive and secretory responses. However, the pre‐treatment of epithelia with ZnSO₄in vitro led to a small but significant decrease in both absorptive and secretory capacities (p < 0.05), with an exception for carbachol (p = 0.07). The results showed that, in piglets, chronic supplementation with zinc did not sustainably influence the jejunal transport properties in the post‐weaning phase. Because transport properties are influenced by the addition of zinc in vitro, we suggest that possible epithelial effects of zinc depend on the acute presence of this ion.</description><subject>absorption</subject><subject>acute effects</subject><subject>age</subject><subject>Aging</subject><subject>Animal Feed - analysis</subject><subject>Animal Nutritional Physiological Phenomena</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>carbachol</subject><subject>chloride secretion</subject><subject>diarrhea</subject><subject>Diet - veterinary</subject><subject>dietary mineral supplements</subject><subject>Dietary Supplements</subject><subject>Dose-Response Relationship, Drug</subject><subject>enterotoxins</subject><subject>epithelium</subject><subject>Escherichia coli</subject><subject>glucose</subject><subject>glutamine</subject><subject>heat stability</subject><subject>intestinal mucosa</subject><subject>ion transport</subject><subject>jejunum</subject><subject>Jejunum - drug effects</subject><subject>Jejunum - metabolism</subject><subject>nutrients</subject><subject>pig</subject><subject>piglets</subject><subject>prostaglandins</subject><subject>secretion</subject><subject>small intestine</subject><subject>Swine - physiology</subject><subject>Ussing chamber</subject><subject>zinc</subject><subject>zinc oxide</subject><subject>Zinc Oxide - administration & dosage</subject><subject>Zinc Oxide - pharmacology</subject><issn>0931-2439</issn><issn>1439-0396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U1rFTEUBuAgFnutLvwDGnCji2nzNZPJUmp7bS3VVou4CpnMmWum82WSQeuvN7fTdiEYAoHDc14OJwi9oGSfpnPQTsM-ZYyzR2hFBVcZ4ap4jFZEcZqxVNhFT0NoCaEyJ8UTtMvyJARVK_T9qGnAxoDHBpsNYDPU-I8bLA7zNHXQwxBNdOOA043eDGEafcSTHyfw0UHALtV_AG6hnYe538ZMbtNBDM_QTmO6AM_v3j10dXz09fBDdvZpfXL47iyzguYsY6WAHIixIucNLwtuZM1N3RSyYpUU1FaKMeCqppxVtGJGlaa2loIsmgpEzffQmyU3DfVzhhB174KFrjMDjHPQtJB5KaUoVKKv_6HtOPshTbdVolRUCJrU20VZP4bgodGTd73xN5oSvd23TvvWt_tO9uVd4lz1UD_I-wUncLCAX66Dm_8n6dPP5_eR2dLhQoTfDx3GX-tCcpnrb-drffnx4mLNLo_1--RfLb4xozYb74K--sIILdJ3k1wyxf8CkneiTQ</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>Gefeller, E. M</creator><creator>Martens, H</creator><creator>Aschenbach, J. R</creator><creator>Klingspor, S</creator><creator>Twardziok, S</creator><creator>Wrede, P</creator><creator>Pieper, R</creator><creator>Lodemann, U</creator><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201506</creationdate><title>Effects of age and zinc supplementation on transport properties in the jejunum of piglets</title><author>Gefeller, E. M ; Martens, H ; Aschenbach, J. R ; Klingspor, S ; Twardziok, S ; Wrede, P ; Pieper, R ; Lodemann, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4152-284e5e0ac453f3863a7d3adf67b2b741cb922e39d132b1b2a98adcc1e76fbe4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>absorption</topic><topic>acute effects</topic><topic>age</topic><topic>Aging</topic><topic>Animal Feed - analysis</topic><topic>Animal Nutritional Physiological Phenomena</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>carbachol</topic><topic>chloride secretion</topic><topic>diarrhea</topic><topic>Diet - veterinary</topic><topic>dietary mineral supplements</topic><topic>Dietary Supplements</topic><topic>Dose-Response Relationship, Drug</topic><topic>enterotoxins</topic><topic>epithelium</topic><topic>Escherichia coli</topic><topic>glucose</topic><topic>glutamine</topic><topic>heat stability</topic><topic>intestinal mucosa</topic><topic>ion transport</topic><topic>jejunum</topic><topic>Jejunum - drug effects</topic><topic>Jejunum - metabolism</topic><topic>nutrients</topic><topic>pig</topic><topic>piglets</topic><topic>prostaglandins</topic><topic>secretion</topic><topic>small intestine</topic><topic>Swine - physiology</topic><topic>Ussing chamber</topic><topic>zinc</topic><topic>zinc oxide</topic><topic>Zinc Oxide - administration & dosage</topic><topic>Zinc Oxide - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gefeller, E. M</creatorcontrib><creatorcontrib>Martens, H</creatorcontrib><creatorcontrib>Aschenbach, J. 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M</au><au>Martens, H</au><au>Aschenbach, J. R</au><au>Klingspor, S</au><au>Twardziok, S</au><au>Wrede, P</au><au>Pieper, R</au><au>Lodemann, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of age and zinc supplementation on transport properties in the jejunum of piglets</atitle><jtitle>Journal of animal physiology and animal nutrition</jtitle><addtitle>J Anim Physiol Anim Nutr</addtitle><date>2015-06</date><risdate>2015</risdate><volume>99</volume><issue>3</issue><spage>542</spage><epage>552</epage><pages>542-552</pages><issn>0931-2439</issn><eissn>1439-0396</eissn><abstract>Zinc is effective in the prevention and treatment of post‐weaning diarrhoea and in promoting piglet growth. Its effects on the absorption of nutrients and the secretory capacity of the intestinal epithelium are controversial. We investigated the effects of age, dietary pharmacological zinc supplementation and acute zinc exposure in vitro on small‐intestinal transport properties of weaned piglets. We further examined whether the effect of zinc on secretory responses depended on the pathway by which chloride secretion is activated. A total of 96 piglets were weaned at 26 days of age and allocated to diets containing three different levels of zinc oxide (50, 150 and 2500 ppm). At the age of 32, 39, 46 and 53 days, piglets were killed, and isolated epithelia from the mid‐jejunum were used for intestinal transport studies in conventional Ussing chambers, with 23 μm ZnSO₄being added to the serosal side for testing acute effects. Absorptive transport was stimulated by mucosal addition of d‐glucose or l‐glutamine. Secretion was activated by serosal addition of prostaglandin E₂, carbachol or by mucosal application of Escherichia coli heat‐stable enterotoxin (Stₚ). Jejunal transport properties showed significant age‐dependent alterations (p < 0.03). Both absorptive and secretory responses were highest in the youngest piglets (32 d). The dietary zinc supplementation had no significant influence on jejunal absorptive and secretory responses. However, the pre‐treatment of epithelia with ZnSO₄in vitro led to a small but significant decrease in both absorptive and secretory capacities (p < 0.05), with an exception for carbachol (p = 0.07). The results showed that, in piglets, chronic supplementation with zinc did not sustainably influence the jejunal transport properties in the post‐weaning phase. Because transport properties are influenced by the addition of zinc in vitro, we suggest that possible epithelial effects of zinc depend on the acute presence of this ion.</abstract><cop>Germany</cop><pub>Blackwell Science</pub><pmid>25039419</pmid><doi>10.1111/jpn.12232</doi><tpages>11</tpages></addata></record> |
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| subjects | absorption acute effects age Aging Animal Feed - analysis Animal Nutritional Physiological Phenomena Animals Biological Transport carbachol chloride secretion diarrhea Diet - veterinary dietary mineral supplements Dietary Supplements Dose-Response Relationship, Drug enterotoxins epithelium Escherichia coli glucose glutamine heat stability intestinal mucosa ion transport jejunum Jejunum - drug effects Jejunum - metabolism nutrients pig piglets prostaglandins secretion small intestine Swine - physiology Ussing chamber zinc zinc oxide Zinc Oxide - administration & dosage Zinc Oxide - pharmacology |
| title | Effects of age and zinc supplementation on transport properties in the jejunum of piglets |
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