High efficiency Agrobacterium-mediated gene transfer to flax
Using an Agrobacterium tumefaciens binary vector (pAL4404, pBI131), we have demonstrated the transfer of the β-glucuronidase gene into the flax (Linum usitatissimum L.) cultivar Glenelg after selection for kanamycin resistance. The transformed lines were obtained by inoculation and subsequent regene...
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| Veröffentlicht in: | Plant cell reports 1994-02, Vol.13 (5), p.282-285 |
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| creator | MLYNAROVA, L BAUER, M NAP, J.-P PRETOVA, A |
| description | Using an Agrobacterium tumefaciens binary vector (pAL4404, pBI131), we have demonstrated the transfer of the β-glucuronidase gene into the flax (Linum usitatissimum L.) cultivar Glenelg after selection for kanamycin resistance. The transformed lines were obtained by inoculation and subsequent regeneration of hypocotyl segments. The callus that formed on the cut surfaces of the hypocotyl segments was isolated three weeks after infection and was subsequently subcultured to yield shoots. This procedure generated a large number of transgenic shoots over a relatively short period of time. The transformation efficiencies obtained were the highest reported so far for this plant species. |
| doi_str_mv | 10.1007/bf00233320 |
| format | Article |
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The transformed lines were obtained by inoculation and subsequent regeneration of hypocotyl segments. The callus that formed on the cut surfaces of the hypocotyl segments was isolated three weeks after infection and was subsequently subcultured to yield shoots. This procedure generated a large number of transgenic shoots over a relatively short period of time. The transformation efficiencies obtained were the highest reported so far for this plant species.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/bf00233320</identifier><identifier>PMID: 24193765</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agrobacterium tumefaciens ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Linum usitatissimum ; Methods. Procedures. 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| ispartof | Plant cell reports, 1994-02, Vol.13 (5), p.282-285 |
| issn | 0721-7714 1432-203X |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Agrobacterium tumefaciens Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Linum usitatissimum Methods. Procedures. Technologies Transgenic animals and transgenic plants Transgenic plants |
| title | High efficiency Agrobacterium-mediated gene transfer to flax |
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