HLA- DR Alleles in Pakistani Patients of Pemphigus Vulgaris
To determine frequency of HLA-DR alleles in Pakistani patients of pemphigus vulgaris in comparison with local healthy controls. Cross-sectional, comparative study. Department of Immunology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from January 2011 to January 2014. Twenty eight patien...
Gespeichert in:
| Veröffentlicht in: | Journal of the College of Physicians and Surgeons--Pakistan 2015-04, Vol.25 (4), p.233-236 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 236 |
|---|---|
| container_issue | 4 |
| container_start_page | 233 |
| container_title | Journal of the College of Physicians and Surgeons--Pakistan |
| container_volume | 25 |
| creator | Khan, Sara Waqar Iftikhar, Nadia Ahmed, Tahir Aziz Bashir, Mukarram |
| description | To determine frequency of HLA-DR alleles in Pakistani patients of pemphigus vulgaris in comparison with local healthy controls.
Cross-sectional, comparative study.
Department of Immunology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from January 2011 to January 2014.
Twenty eight patients with biopsy proven diagnosis of pemphigus vulgaris referred from Department of Dermatology, Military Hospital, Rawalpindi were included. Patients were compared with a group of 150 unrelated local healthy subjects. DNA was extracted from peripheral blood collected in Tri-potassium EDTA. HLA-DRB1 typing was carried out on allele level (DRB1*01--DRB1*16) using SSP (sequence specific primers). HLA type was determined by agarose gel electrophoresis and results recorded. Phenotype frequency of various alleles among patient group and control group was calculated by direct counting and significance of their association was determined by Fisher's exact test/ Chi square test.
A total of 12 male and 16 female patients, with age ranging from 21 to 34 (mean 23.4 years) were genotyped for HLA-DRB1 loci. A statistically significant association of the disease with HLA-DRB1*04 was observed (50% versus 20.7% in controls, p < 0.05).
There is a strong association of HLA-DRB1*04 with pemphigus vulgaris in Pakistani population. |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1675167834</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A408783383</galeid><sourcerecordid>A408783383</sourcerecordid><originalsourceid>FETCH-LOGICAL-g193t-c684e5d9f6647cd795f1ece62f387bc2c183dcf6f6df66e6c9d6ff9ba9ae8db93</originalsourceid><addsrcrecordid>eNptkE1LxDAQhoMo7rr6FyTgxUulado0wVNZV1dYcBEVbyVNJjWafti0B_-9kV0PgszXy_DMMMwBmhPGSZSHfBh0nCQR5ex1hk68f49jmhHOj9EsybgQhKdzdL3eFBG-ecSFc-DAY9virfywfpStDWq00I4edwZvoenfbD15_DK5Wg7Wn6IjI52Hs31doOfb1dNyHW0e7u6XxSaqiaBjpBhPIdPCMJbmSuciMwQUsMRQnlcqUYRTrQwzTAcEmBKaGSMqKSRwXQm6QJe7vf3QfU7gx7KxXoFzsoVu8iVheRaC0zSgFzu0lg5K25puHKT6wcsijXlgaPAFuvqHCqahsaprwdjQ_zNwvr9gqhrQZT_YRg5f5e8f6TfE5G29</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1675167834</pqid></control><display><type>article</type><title>HLA- DR Alleles in Pakistani Patients of Pemphigus Vulgaris</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Khan, Sara Waqar ; Iftikhar, Nadia ; Ahmed, Tahir Aziz ; Bashir, Mukarram</creator><creatorcontrib>Khan, Sara Waqar ; Iftikhar, Nadia ; Ahmed, Tahir Aziz ; Bashir, Mukarram</creatorcontrib><description>To determine frequency of HLA-DR alleles in Pakistani patients of pemphigus vulgaris in comparison with local healthy controls.
Cross-sectional, comparative study.
Department of Immunology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from January 2011 to January 2014.
Twenty eight patients with biopsy proven diagnosis of pemphigus vulgaris referred from Department of Dermatology, Military Hospital, Rawalpindi were included. Patients were compared with a group of 150 unrelated local healthy subjects. DNA was extracted from peripheral blood collected in Tri-potassium EDTA. HLA-DRB1 typing was carried out on allele level (DRB1*01--DRB1*16) using SSP (sequence specific primers). HLA type was determined by agarose gel electrophoresis and results recorded. Phenotype frequency of various alleles among patient group and control group was calculated by direct counting and significance of their association was determined by Fisher's exact test/ Chi square test.
A total of 12 male and 16 female patients, with age ranging from 21 to 34 (mean 23.4 years) were genotyped for HLA-DRB1 loci. A statistically significant association of the disease with HLA-DRB1*04 was observed (50% versus 20.7% in controls, p < 0.05).
There is a strong association of HLA-DRB1*04 with pemphigus vulgaris in Pakistani population.</description><identifier>ISSN: 1022-386X</identifier><identifier>EISSN: 1681-7168</identifier><identifier>PMID: 25899184</identifier><language>eng</language><publisher>Pakistan: College of Physicians and Surgeons Pakistan</publisher><subject>Adult ; Alleles ; Biopsy ; Cross-Sectional Studies ; DNA - genetics ; Female ; Follow-Up Studies ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Health aspects ; HLA antigens ; HLA-DR Antigens - genetics ; Humans ; Incidence ; Male ; Pakistan ; Pemphigus ; Pemphigus - epidemiology ; Pemphigus - genetics ; Pemphigus - pathology ; Physiological aspects ; Retrospective Studies ; Young Adult</subject><ispartof>Journal of the College of Physicians and Surgeons--Pakistan, 2015-04, Vol.25 (4), p.233-236</ispartof><rights>COPYRIGHT 2015 College of Physicians and Surgeons Pakistan</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25899184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Sara Waqar</creatorcontrib><creatorcontrib>Iftikhar, Nadia</creatorcontrib><creatorcontrib>Ahmed, Tahir Aziz</creatorcontrib><creatorcontrib>Bashir, Mukarram</creatorcontrib><title>HLA- DR Alleles in Pakistani Patients of Pemphigus Vulgaris</title><title>Journal of the College of Physicians and Surgeons--Pakistan</title><addtitle>J Coll Physicians Surg Pak</addtitle><description>To determine frequency of HLA-DR alleles in Pakistani patients of pemphigus vulgaris in comparison with local healthy controls.
Cross-sectional, comparative study.
Department of Immunology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from January 2011 to January 2014.
Twenty eight patients with biopsy proven diagnosis of pemphigus vulgaris referred from Department of Dermatology, Military Hospital, Rawalpindi were included. Patients were compared with a group of 150 unrelated local healthy subjects. DNA was extracted from peripheral blood collected in Tri-potassium EDTA. HLA-DRB1 typing was carried out on allele level (DRB1*01--DRB1*16) using SSP (sequence specific primers). HLA type was determined by agarose gel electrophoresis and results recorded. Phenotype frequency of various alleles among patient group and control group was calculated by direct counting and significance of their association was determined by Fisher's exact test/ Chi square test.
A total of 12 male and 16 female patients, with age ranging from 21 to 34 (mean 23.4 years) were genotyped for HLA-DRB1 loci. A statistically significant association of the disease with HLA-DRB1*04 was observed (50% versus 20.7% in controls, p < 0.05).
There is a strong association of HLA-DRB1*04 with pemphigus vulgaris in Pakistani population.</description><subject>Adult</subject><subject>Alleles</subject><subject>Biopsy</subject><subject>Cross-Sectional Studies</subject><subject>DNA - genetics</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gene Frequency</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotype</subject><subject>Health aspects</subject><subject>HLA antigens</subject><subject>HLA-DR Antigens - genetics</subject><subject>Humans</subject><subject>Incidence</subject><subject>Male</subject><subject>Pakistan</subject><subject>Pemphigus</subject><subject>Pemphigus - epidemiology</subject><subject>Pemphigus - genetics</subject><subject>Pemphigus - pathology</subject><subject>Physiological aspects</subject><subject>Retrospective Studies</subject><subject>Young Adult</subject><issn>1022-386X</issn><issn>1681-7168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1LxDAQhoMo7rr6FyTgxUulado0wVNZV1dYcBEVbyVNJjWafti0B_-9kV0PgszXy_DMMMwBmhPGSZSHfBh0nCQR5ex1hk68f49jmhHOj9EsybgQhKdzdL3eFBG-ecSFc-DAY9virfywfpStDWq00I4edwZvoenfbD15_DK5Wg7Wn6IjI52Hs31doOfb1dNyHW0e7u6XxSaqiaBjpBhPIdPCMJbmSuciMwQUsMRQnlcqUYRTrQwzTAcEmBKaGSMqKSRwXQm6QJe7vf3QfU7gx7KxXoFzsoVu8iVheRaC0zSgFzu0lg5K25puHKT6wcsijXlgaPAFuvqHCqahsaprwdjQ_zNwvr9gqhrQZT_YRg5f5e8f6TfE5G29</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Khan, Sara Waqar</creator><creator>Iftikhar, Nadia</creator><creator>Ahmed, Tahir Aziz</creator><creator>Bashir, Mukarram</creator><general>College of Physicians and Surgeons Pakistan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201504</creationdate><title>HLA- DR Alleles in Pakistani Patients of Pemphigus Vulgaris</title><author>Khan, Sara Waqar ; Iftikhar, Nadia ; Ahmed, Tahir Aziz ; Bashir, Mukarram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g193t-c684e5d9f6647cd795f1ece62f387bc2c183dcf6f6df66e6c9d6ff9ba9ae8db93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Alleles</topic><topic>Biopsy</topic><topic>Cross-Sectional Studies</topic><topic>DNA - genetics</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Gene Frequency</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotype</topic><topic>Health aspects</topic><topic>HLA antigens</topic><topic>HLA-DR Antigens - genetics</topic><topic>Humans</topic><topic>Incidence</topic><topic>Male</topic><topic>Pakistan</topic><topic>Pemphigus</topic><topic>Pemphigus - epidemiology</topic><topic>Pemphigus - genetics</topic><topic>Pemphigus - pathology</topic><topic>Physiological aspects</topic><topic>Retrospective Studies</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Sara Waqar</creatorcontrib><creatorcontrib>Iftikhar, Nadia</creatorcontrib><creatorcontrib>Ahmed, Tahir Aziz</creatorcontrib><creatorcontrib>Bashir, Mukarram</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Sara Waqar</au><au>Iftikhar, Nadia</au><au>Ahmed, Tahir Aziz</au><au>Bashir, Mukarram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HLA- DR Alleles in Pakistani Patients of Pemphigus Vulgaris</atitle><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle><addtitle>J Coll Physicians Surg Pak</addtitle><date>2015-04</date><risdate>2015</risdate><volume>25</volume><issue>4</issue><spage>233</spage><epage>236</epage><pages>233-236</pages><issn>1022-386X</issn><eissn>1681-7168</eissn><abstract>To determine frequency of HLA-DR alleles in Pakistani patients of pemphigus vulgaris in comparison with local healthy controls.
Cross-sectional, comparative study.
Department of Immunology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from January 2011 to January 2014.
Twenty eight patients with biopsy proven diagnosis of pemphigus vulgaris referred from Department of Dermatology, Military Hospital, Rawalpindi were included. Patients were compared with a group of 150 unrelated local healthy subjects. DNA was extracted from peripheral blood collected in Tri-potassium EDTA. HLA-DRB1 typing was carried out on allele level (DRB1*01--DRB1*16) using SSP (sequence specific primers). HLA type was determined by agarose gel electrophoresis and results recorded. Phenotype frequency of various alleles among patient group and control group was calculated by direct counting and significance of their association was determined by Fisher's exact test/ Chi square test.
A total of 12 male and 16 female patients, with age ranging from 21 to 34 (mean 23.4 years) were genotyped for HLA-DRB1 loci. A statistically significant association of the disease with HLA-DRB1*04 was observed (50% versus 20.7% in controls, p < 0.05).
There is a strong association of HLA-DRB1*04 with pemphigus vulgaris in Pakistani population.</abstract><cop>Pakistan</cop><pub>College of Physicians and Surgeons Pakistan</pub><pmid>25899184</pmid><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1022-386X |
| ispartof | Journal of the College of Physicians and Surgeons--Pakistan, 2015-04, Vol.25 (4), p.233-236 |
| issn | 1022-386X 1681-7168 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1675167834 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Alleles Biopsy Cross-Sectional Studies DNA - genetics Female Follow-Up Studies Gene Frequency Genetic Predisposition to Disease Genotype Health aspects HLA antigens HLA-DR Antigens - genetics Humans Incidence Male Pakistan Pemphigus Pemphigus - epidemiology Pemphigus - genetics Pemphigus - pathology Physiological aspects Retrospective Studies Young Adult |
| title | HLA- DR Alleles in Pakistani Patients of Pemphigus Vulgaris |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T09%3A21%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HLA-%20DR%20Alleles%20in%20Pakistani%20Patients%20of%20Pemphigus%20Vulgaris&rft.jtitle=Journal%20of%20the%20College%20of%20Physicians%20and%20Surgeons--Pakistan&rft.au=Khan,%20Sara%20Waqar&rft.date=2015-04&rft.volume=25&rft.issue=4&rft.spage=233&rft.epage=236&rft.pages=233-236&rft.issn=1022-386X&rft.eissn=1681-7168&rft_id=info:doi/&rft_dat=%3Cgale_proqu%3EA408783383%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1675167834&rft_id=info:pmid/25899184&rft_galeid=A408783383&rfr_iscdi=true |