Marker exchange mutagenesis of the hydN genes in Desulfovibrio fructosovorans

Summary A strain of Desulfovibrio fructosovorans deleted from the hydN [NiFe]hydrogenase structural gene was constructed. A plasmid carrying a 7 kb DNA fragment on which the hydN gene had been replaced by the npt reporter gene (kanamycin‐resistant, KnR) was introduced into D. fructosovorans by elect...

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