Determination of S-Benzyl-N-acetyl-l-cysteine by Gas Chromatography/Mass Spectrometry as a New Marker of Toluene Exposure
We investigated by gas chromatography/mass spectrometry if S-benzyl-N-acetyl-l-cysteine (SBAC) is excreted into the urine of six printing workers and 20 sniffers after exposure to toluene. SBAC was extracted from 10 mL urine with chloroform and back extracted into 1M sodium bicarbonate. After acidfy...
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| Veröffentlicht in: | Journal of analytical toxicology 1994-03, Vol.18 (2), p.78-80 |
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| container_title | Journal of analytical toxicology |
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| creator | Takahashi, Setsunori Kagawa, Masato Shiwaku, Kuninori Matsubara, Kazuo |
| description | We investigated by gas chromatography/mass spectrometry if S-benzyl-N-acetyl-l-cysteine (SBAC) is excreted into the urine of six printing workers and 20 sniffers after exposure to toluene. SBAC was extracted from 10 mL urine with chloroform and back extracted into 1M sodium bicarbonate. After acidfying, the sample was reextracted with ethyl acetate. The SBAC in urine was also measured by selected ion monitoring methods, using deuterium-labeled SBAC as an internal standard. An excellent linearity was obtained from 0.01 to 3.0 mg/L (r = 0.998). Urinary concentrations of SBAC were determined quantitatively in all samples from sniffers and printing workers (n = 26, 0.02–43.29 mg/L) but were not determined in the urine of persons not exposed to toluene (n = 40). The results of this experiment indicate that SBAC is excreted as a minor metabolite of toluene in human urine. The analysis of urinary SBAC is a useful tool in identifying toluene exposure. |
| doi_str_mv | 10.1093/jat/18.2.78 |
| format | Article |
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SBAC was extracted from 10 mL urine with chloroform and back extracted into 1M sodium bicarbonate. After acidfying, the sample was reextracted with ethyl acetate. The SBAC in urine was also measured by selected ion monitoring methods, using deuterium-labeled SBAC as an internal standard. An excellent linearity was obtained from 0.01 to 3.0 mg/L (r = 0.998). Urinary concentrations of SBAC were determined quantitatively in all samples from sniffers and printing workers (n = 26, 0.02–43.29 mg/L) but were not determined in the urine of persons not exposed to toluene (n = 40). The results of this experiment indicate that SBAC is excreted as a minor metabolite of toluene in human urine. The analysis of urinary SBAC is a useful tool in identifying toluene exposure.</description><identifier>ISSN: 0146-4760</identifier><identifier>EISSN: 1945-2403</identifier><identifier>DOI: 10.1093/jat/18.2.78</identifier><identifier>PMID: 8207937</identifier><identifier>CODEN: JATOD3</identifier><language>eng</language><publisher>Niles, IL: Oxford University Press</publisher><subject>Acetylcysteine - analogs & derivatives ; Acetylcysteine - urine ; Biological and medical sciences ; Chemical and industrial products toxicology. Toxic occupational diseases ; Chloroform ; Ethyl acetate ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Humans ; Mass spectroscopy ; Medical sciences ; Metabolites ; Occupational Exposure ; Printing ; Sodium bicarbonate ; Solvents ; Substance-Related Disorders - urine ; Toluene ; Toluene - metabolism ; Toxicology ; Urine</subject><ispartof>Journal of analytical toxicology, 1994-03, Vol.18 (2), p.78-80</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-3dd0e1e3dfe03e405057fe24f390b3be9702cff2204ec411db149676b8bf2ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3984741$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8207937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takahashi, Setsunori</creatorcontrib><creatorcontrib>Kagawa, Masato</creatorcontrib><creatorcontrib>Shiwaku, Kuninori</creatorcontrib><creatorcontrib>Matsubara, Kazuo</creatorcontrib><title>Determination of S-Benzyl-N-acetyl-l-cysteine by Gas Chromatography/Mass Spectrometry as a New Marker of Toluene Exposure</title><title>Journal of analytical toxicology</title><addtitle>Journal of Analytical Toxicology</addtitle><description>We investigated by gas chromatography/mass spectrometry if S-benzyl-N-acetyl-l-cysteine (SBAC) is excreted into the urine of six printing workers and 20 sniffers after exposure to toluene. SBAC was extracted from 10 mL urine with chloroform and back extracted into 1M sodium bicarbonate. After acidfying, the sample was reextracted with ethyl acetate. The SBAC in urine was also measured by selected ion monitoring methods, using deuterium-labeled SBAC as an internal standard. An excellent linearity was obtained from 0.01 to 3.0 mg/L (r = 0.998). Urinary concentrations of SBAC were determined quantitatively in all samples from sniffers and printing workers (n = 26, 0.02–43.29 mg/L) but were not determined in the urine of persons not exposed to toluene (n = 40). The results of this experiment indicate that SBAC is excreted as a minor metabolite of toluene in human urine. The analysis of urinary SBAC is a useful tool in identifying toluene exposure.</description><subject>Acetylcysteine - analogs & derivatives</subject><subject>Acetylcysteine - urine</subject><subject>Biological and medical sciences</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Chloroform</subject><subject>Ethyl acetate</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>Mass spectroscopy</subject><subject>Medical sciences</subject><subject>Metabolites</subject><subject>Occupational Exposure</subject><subject>Printing</subject><subject>Sodium bicarbonate</subject><subject>Solvents</subject><subject>Substance-Related Disorders - urine</subject><subject>Toluene</subject><subject>Toluene - metabolism</subject><subject>Toxicology</subject><subject>Urine</subject><issn>0146-4760</issn><issn>1945-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxS0EKtuFE2ekHFCFhLxrx46dHGHpB2K3ldqVQL1YTjKmKUmc2o7a8Nfjsqs9cvLI7zdPmvcQekfJgpKCLe91WNJ8kS5k_gLNaMEznHLCXqIZoVxgLgV5jY69vyeEilywI3SUp0QWTM7Q9BUCuK7pdWhsn1iT3OAv0P-ZWnyJdQUhDi2uJh-g6SEpp-Rc-2R152yng_3l9HA3LTfa--RmgCrEbwhuSiKjk0t4TDba_Qb37Lu17QjR4vRpsH508Aa9Mrr18Hb_ztH27HS7usDrq_Nvq89rXHGaBczqmgAFVhsgDDjJSCYNpNywgpSshEKStDImTQmHuEHrkvJCSFHmpUl1yeboZGc7OPswgg-qa3wFbat7sKNXVEguWQxjjj7-H4xRcyFE8Yx-2qGVs947MGpwTafdFKF_nIqVKJqrVMk80u_3xmPZQX1g9x1E_cNe177SrXG6rxp_wFiRc8lpxPAOa2IXTwc55qtEPCBTFz9vVcFuv5_9WF-rDfsLntGkMQ</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>Takahashi, Setsunori</creator><creator>Kagawa, Masato</creator><creator>Shiwaku, Kuninori</creator><creator>Matsubara, Kazuo</creator><general>Oxford University Press</general><general>Preston</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19940301</creationdate><title>Determination of S-Benzyl-N-acetyl-l-cysteine by Gas Chromatography/Mass Spectrometry as a New Marker of Toluene Exposure</title><author>Takahashi, Setsunori ; Kagawa, Masato ; Shiwaku, Kuninori ; Matsubara, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-3dd0e1e3dfe03e405057fe24f390b3be9702cff2204ec411db149676b8bf2ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acetylcysteine - analogs & derivatives</topic><topic>Acetylcysteine - urine</topic><topic>Biological and medical sciences</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Chloroform</topic><topic>Ethyl acetate</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>Mass spectroscopy</topic><topic>Medical sciences</topic><topic>Metabolites</topic><topic>Occupational Exposure</topic><topic>Printing</topic><topic>Sodium bicarbonate</topic><topic>Solvents</topic><topic>Substance-Related Disorders - urine</topic><topic>Toluene</topic><topic>Toluene - metabolism</topic><topic>Toxicology</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takahashi, Setsunori</creatorcontrib><creatorcontrib>Kagawa, Masato</creatorcontrib><creatorcontrib>Shiwaku, Kuninori</creatorcontrib><creatorcontrib>Matsubara, Kazuo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of analytical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takahashi, Setsunori</au><au>Kagawa, Masato</au><au>Shiwaku, Kuninori</au><au>Matsubara, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of S-Benzyl-N-acetyl-l-cysteine by Gas Chromatography/Mass Spectrometry as a New Marker of Toluene Exposure</atitle><jtitle>Journal of analytical toxicology</jtitle><addtitle>Journal of Analytical Toxicology</addtitle><date>1994-03-01</date><risdate>1994</risdate><volume>18</volume><issue>2</issue><spage>78</spage><epage>80</epage><pages>78-80</pages><issn>0146-4760</issn><eissn>1945-2403</eissn><coden>JATOD3</coden><abstract>We investigated by gas chromatography/mass spectrometry if S-benzyl-N-acetyl-l-cysteine (SBAC) is excreted into the urine of six printing workers and 20 sniffers after exposure to toluene. SBAC was extracted from 10 mL urine with chloroform and back extracted into 1M sodium bicarbonate. After acidfying, the sample was reextracted with ethyl acetate. The SBAC in urine was also measured by selected ion monitoring methods, using deuterium-labeled SBAC as an internal standard. An excellent linearity was obtained from 0.01 to 3.0 mg/L (r = 0.998). Urinary concentrations of SBAC were determined quantitatively in all samples from sniffers and printing workers (n = 26, 0.02–43.29 mg/L) but were not determined in the urine of persons not exposed to toluene (n = 40). The results of this experiment indicate that SBAC is excreted as a minor metabolite of toluene in human urine. The analysis of urinary SBAC is a useful tool in identifying toluene exposure.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>8207937</pmid><doi>10.1093/jat/18.2.78</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0146-4760 |
| ispartof | Journal of analytical toxicology, 1994-03, Vol.18 (2), p.78-80 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; Alma/SFX Local Collection |
| subjects | Acetylcysteine - analogs & derivatives Acetylcysteine - urine Biological and medical sciences Chemical and industrial products toxicology. Toxic occupational diseases Chloroform Ethyl acetate Gas chromatography Gas Chromatography-Mass Spectrometry Humans Mass spectroscopy Medical sciences Metabolites Occupational Exposure Printing Sodium bicarbonate Solvents Substance-Related Disorders - urine Toluene Toluene - metabolism Toxicology Urine |
| title | Determination of S-Benzyl-N-acetyl-l-cysteine by Gas Chromatography/Mass Spectrometry as a New Marker of Toluene Exposure |
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