Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia
Background Heavy/light chain assay allows the characterization and quantification of immunoglobulin light chains bound to heavy chains for each Ig’k and Ig’λ immunoglobulin class, discriminating between the involved/uninvolved isotypes in plasma cell dyscrasia. The Ig’k/Ig’λ ratio (heavy/light chain...
Gespeichert in:
| Veröffentlicht in: | Annals of clinical biochemistry 2015-05, Vol.52 (3), p.337-345 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 345 |
|---|---|
| container_issue | 3 |
| container_start_page | 337 |
| container_title | Annals of clinical biochemistry |
| container_volume | 52 |
| creator | Paolini, Lucia Di Noto, Giuseppe Maffina, Francesca Martellosio, Giovanni Radeghieri, Annalisa Luigi, Caimi Ricotta, Doris |
| description | Background
Heavy/light chain assay allows the characterization and quantification of immunoglobulin light chains bound to heavy chains for each Ig’k and Ig’λ immunoglobulin class, discriminating between the involved/uninvolved isotypes in plasma cell dyscrasia. The Ig’k/Ig’λ ratio (heavy/light chain ratio) enables to monitor the trend of monoclonal component during therapy and disease evolution.
Objective
In this study, we evaluate the impact of the heavy/light chain assay in monitoring multiple myeloma patients in comparison with conventional techniques.
Methods
Serum samples of 28 patients with IgG or IgA monoclonal component were collected for a mean of 109 days and analyzed. The heavy/light chain assay was compared with classical immunoglobulin quantification (Ig’Tot), serum immunofixation electrophoresis, serum protein electrophoresis, and serum-free light chains quantification. Serum samples from 30 healthy patients were used as control (polyclonal).
Results
Heavy/light chain ratio and serum immunofixation electrophoresis were comparable in 86% of the cases, and free light chain ratio and heavy/light chain ratio in 71.8%. Heavy/light chain assay and Ig’Tot measurements showed a concentration-dependent agreement in monoclonal patients. The heavy/light chain assay was able to quantify the monoclonal component migrating in SPE β region: this occurred in 10% of our IgG and 50% of our IgA patients.
Conclusions
The concordance scores indicate that heavy/light chain and Ig’Tot assays show differences at high monoclonal component values. The heavy/light chain ratio, serum immunofixation electrophoresis, and free light chain ratio showed partial concordance. Our study confirmed that, in the context of heavy/light chain assay, heavy/light chain Ig’k and Ig’λ absolute values and heavy/light chain ratio are both important tools to monitor the presence of monoclonal component that are difficult to be identified in SPE. |
| doi_str_mv | 10.1177/0004563214564225 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1674687142</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_0004563214564225</sage_id><sourcerecordid>1674687142</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-5c390824b88f44a493d95826876ca7b0c00677096c3ae0ae144796430d848eb3</originalsourceid><addsrcrecordid>eNp1kE9LxDAQxYMouv65e5IcvVQnbdqkR1n8B4IX72U2TXcjbVMzrbJHwU_iR_OT2LrqQfAywzDvPR4_xo4FnAmh1DkAyDRLYjFOGcfpFpsJlepIAIhtNpve0fTfY_tEj-MZK4BdthenMtN5rmbsde6bDoMj33Jf8Rv7vK5dbz_e3vnt8oJjW477miMRrvmL61fc-PbZtr3zLda8t2bVuqfBEq984P3K8tLhsvXk6Mtc-br2L9HQTeldjdQgN7auebkmE5AcHrKdCmuyR9_7gD1cXT7Mb6K7--vb-cVdZBKV91Fqkhx0LBdaV1KizJMyT3WcaZUZVAswAJlSkGcmQQtohZQqz2QCpZbaLpIDdrqJ7YKf-vZF42hqgq31AxUiUyMTJWQ8SmEjNcETBVsVXXANhnUhoJi4F3-5j5aT7_Rh0djy1_ADehREGwHh0haPfggjPvo_8BMXXYs6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1674687142</pqid></control><display><type>article</type><title>Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia</title><source>MEDLINE</source><source>SAGE Complete A-Z List</source><creator>Paolini, Lucia ; Di Noto, Giuseppe ; Maffina, Francesca ; Martellosio, Giovanni ; Radeghieri, Annalisa ; Luigi, Caimi ; Ricotta, Doris</creator><creatorcontrib>Paolini, Lucia ; Di Noto, Giuseppe ; Maffina, Francesca ; Martellosio, Giovanni ; Radeghieri, Annalisa ; Luigi, Caimi ; Ricotta, Doris</creatorcontrib><description>Background
Heavy/light chain assay allows the characterization and quantification of immunoglobulin light chains bound to heavy chains for each Ig’k and Ig’λ immunoglobulin class, discriminating between the involved/uninvolved isotypes in plasma cell dyscrasia. The Ig’k/Ig’λ ratio (heavy/light chain ratio) enables to monitor the trend of monoclonal component during therapy and disease evolution.
Objective
In this study, we evaluate the impact of the heavy/light chain assay in monitoring multiple myeloma patients in comparison with conventional techniques.
Methods
Serum samples of 28 patients with IgG or IgA monoclonal component were collected for a mean of 109 days and analyzed. The heavy/light chain assay was compared with classical immunoglobulin quantification (Ig’Tot), serum immunofixation electrophoresis, serum protein electrophoresis, and serum-free light chains quantification. Serum samples from 30 healthy patients were used as control (polyclonal).
Results
Heavy/light chain ratio and serum immunofixation electrophoresis were comparable in 86% of the cases, and free light chain ratio and heavy/light chain ratio in 71.8%. Heavy/light chain assay and Ig’Tot measurements showed a concentration-dependent agreement in monoclonal patients. The heavy/light chain assay was able to quantify the monoclonal component migrating in SPE β region: this occurred in 10% of our IgG and 50% of our IgA patients.
Conclusions
The concordance scores indicate that heavy/light chain and Ig’Tot assays show differences at high monoclonal component values. The heavy/light chain ratio, serum immunofixation electrophoresis, and free light chain ratio showed partial concordance. Our study confirmed that, in the context of heavy/light chain assay, heavy/light chain Ig’k and Ig’λ absolute values and heavy/light chain ratio are both important tools to monitor the presence of monoclonal component that are difficult to be identified in SPE.</description><identifier>ISSN: 0004-5632</identifier><identifier>EISSN: 1758-1001</identifier><identifier>DOI: 10.1177/0004563214564225</identifier><identifier>PMID: 25468997</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Electrophoresis - methods ; Electrophoresis - standards ; Follow-Up Studies ; Humans ; Immunoglobulin A - blood ; Immunoglobulin G - blood ; Paraproteinemias - blood ; Paraproteinemias - diagnosis</subject><ispartof>Annals of clinical biochemistry, 2015-05, Vol.52 (3), p.337-345</ispartof><rights>The Author(s) 2015 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav</rights><rights>The Author(s) 2015 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-5c390824b88f44a493d95826876ca7b0c00677096c3ae0ae144796430d848eb3</citedby><cites>FETCH-LOGICAL-c379t-5c390824b88f44a493d95826876ca7b0c00677096c3ae0ae144796430d848eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0004563214564225$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0004563214564225$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,777,781,21800,27905,27906,43602,43603</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25468997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paolini, Lucia</creatorcontrib><creatorcontrib>Di Noto, Giuseppe</creatorcontrib><creatorcontrib>Maffina, Francesca</creatorcontrib><creatorcontrib>Martellosio, Giovanni</creatorcontrib><creatorcontrib>Radeghieri, Annalisa</creatorcontrib><creatorcontrib>Luigi, Caimi</creatorcontrib><creatorcontrib>Ricotta, Doris</creatorcontrib><title>Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia</title><title>Annals of clinical biochemistry</title><addtitle>Ann Clin Biochem</addtitle><description>Background
Heavy/light chain assay allows the characterization and quantification of immunoglobulin light chains bound to heavy chains for each Ig’k and Ig’λ immunoglobulin class, discriminating between the involved/uninvolved isotypes in plasma cell dyscrasia. The Ig’k/Ig’λ ratio (heavy/light chain ratio) enables to monitor the trend of monoclonal component during therapy and disease evolution.
Objective
In this study, we evaluate the impact of the heavy/light chain assay in monitoring multiple myeloma patients in comparison with conventional techniques.
Methods
Serum samples of 28 patients with IgG or IgA monoclonal component were collected for a mean of 109 days and analyzed. The heavy/light chain assay was compared with classical immunoglobulin quantification (Ig’Tot), serum immunofixation electrophoresis, serum protein electrophoresis, and serum-free light chains quantification. Serum samples from 30 healthy patients were used as control (polyclonal).
Results
Heavy/light chain ratio and serum immunofixation electrophoresis were comparable in 86% of the cases, and free light chain ratio and heavy/light chain ratio in 71.8%. Heavy/light chain assay and Ig’Tot measurements showed a concentration-dependent agreement in monoclonal patients. The heavy/light chain assay was able to quantify the monoclonal component migrating in SPE β region: this occurred in 10% of our IgG and 50% of our IgA patients.
Conclusions
The concordance scores indicate that heavy/light chain and Ig’Tot assays show differences at high monoclonal component values. The heavy/light chain ratio, serum immunofixation electrophoresis, and free light chain ratio showed partial concordance. Our study confirmed that, in the context of heavy/light chain assay, heavy/light chain Ig’k and Ig’λ absolute values and heavy/light chain ratio are both important tools to monitor the presence of monoclonal component that are difficult to be identified in SPE.</description><subject>Electrophoresis - methods</subject><subject>Electrophoresis - standards</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Immunoglobulin A - blood</subject><subject>Immunoglobulin G - blood</subject><subject>Paraproteinemias - blood</subject><subject>Paraproteinemias - diagnosis</subject><issn>0004-5632</issn><issn>1758-1001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE9LxDAQxYMouv65e5IcvVQnbdqkR1n8B4IX72U2TXcjbVMzrbJHwU_iR_OT2LrqQfAywzDvPR4_xo4FnAmh1DkAyDRLYjFOGcfpFpsJlepIAIhtNpve0fTfY_tEj-MZK4BdthenMtN5rmbsde6bDoMj33Jf8Rv7vK5dbz_e3vnt8oJjW477miMRrvmL61fc-PbZtr3zLda8t2bVuqfBEq984P3K8tLhsvXk6Mtc-br2L9HQTeldjdQgN7auebkmE5AcHrKdCmuyR9_7gD1cXT7Mb6K7--vb-cVdZBKV91Fqkhx0LBdaV1KizJMyT3WcaZUZVAswAJlSkGcmQQtohZQqz2QCpZbaLpIDdrqJ7YKf-vZF42hqgq31AxUiUyMTJWQ8SmEjNcETBVsVXXANhnUhoJi4F3-5j5aT7_Rh0djy1_ADehREGwHh0haPfggjPvo_8BMXXYs6</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Paolini, Lucia</creator><creator>Di Noto, Giuseppe</creator><creator>Maffina, Francesca</creator><creator>Martellosio, Giovanni</creator><creator>Radeghieri, Annalisa</creator><creator>Luigi, Caimi</creator><creator>Ricotta, Doris</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150501</creationdate><title>Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia</title><author>Paolini, Lucia ; Di Noto, Giuseppe ; Maffina, Francesca ; Martellosio, Giovanni ; Radeghieri, Annalisa ; Luigi, Caimi ; Ricotta, Doris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-5c390824b88f44a493d95826876ca7b0c00677096c3ae0ae144796430d848eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Electrophoresis - methods</topic><topic>Electrophoresis - standards</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Immunoglobulin A - blood</topic><topic>Immunoglobulin G - blood</topic><topic>Paraproteinemias - blood</topic><topic>Paraproteinemias - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paolini, Lucia</creatorcontrib><creatorcontrib>Di Noto, Giuseppe</creatorcontrib><creatorcontrib>Maffina, Francesca</creatorcontrib><creatorcontrib>Martellosio, Giovanni</creatorcontrib><creatorcontrib>Radeghieri, Annalisa</creatorcontrib><creatorcontrib>Luigi, Caimi</creatorcontrib><creatorcontrib>Ricotta, Doris</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paolini, Lucia</au><au>Di Noto, Giuseppe</au><au>Maffina, Francesca</au><au>Martellosio, Giovanni</au><au>Radeghieri, Annalisa</au><au>Luigi, Caimi</au><au>Ricotta, Doris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia</atitle><jtitle>Annals of clinical biochemistry</jtitle><addtitle>Ann Clin Biochem</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>52</volume><issue>3</issue><spage>337</spage><epage>345</epage><pages>337-345</pages><issn>0004-5632</issn><eissn>1758-1001</eissn><abstract>Background
Heavy/light chain assay allows the characterization and quantification of immunoglobulin light chains bound to heavy chains for each Ig’k and Ig’λ immunoglobulin class, discriminating between the involved/uninvolved isotypes in plasma cell dyscrasia. The Ig’k/Ig’λ ratio (heavy/light chain ratio) enables to monitor the trend of monoclonal component during therapy and disease evolution.
Objective
In this study, we evaluate the impact of the heavy/light chain assay in monitoring multiple myeloma patients in comparison with conventional techniques.
Methods
Serum samples of 28 patients with IgG or IgA monoclonal component were collected for a mean of 109 days and analyzed. The heavy/light chain assay was compared with classical immunoglobulin quantification (Ig’Tot), serum immunofixation electrophoresis, serum protein electrophoresis, and serum-free light chains quantification. Serum samples from 30 healthy patients were used as control (polyclonal).
Results
Heavy/light chain ratio and serum immunofixation electrophoresis were comparable in 86% of the cases, and free light chain ratio and heavy/light chain ratio in 71.8%. Heavy/light chain assay and Ig’Tot measurements showed a concentration-dependent agreement in monoclonal patients. The heavy/light chain assay was able to quantify the monoclonal component migrating in SPE β region: this occurred in 10% of our IgG and 50% of our IgA patients.
Conclusions
The concordance scores indicate that heavy/light chain and Ig’Tot assays show differences at high monoclonal component values. The heavy/light chain ratio, serum immunofixation electrophoresis, and free light chain ratio showed partial concordance. Our study confirmed that, in the context of heavy/light chain assay, heavy/light chain Ig’k and Ig’λ absolute values and heavy/light chain ratio are both important tools to monitor the presence of monoclonal component that are difficult to be identified in SPE.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>25468997</pmid><doi>10.1177/0004563214564225</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0004-5632 |
| ispartof | Annals of clinical biochemistry, 2015-05, Vol.52 (3), p.337-345 |
| issn | 0004-5632 1758-1001 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1674687142 |
| source | MEDLINE; SAGE Complete A-Z List |
| subjects | Electrophoresis - methods Electrophoresis - standards Follow-Up Studies Humans Immunoglobulin A - blood Immunoglobulin G - blood Paraproteinemias - blood Paraproteinemias - diagnosis |
| title | Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T19%3A11%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20Hevylite%E2%84%A2%20IgA%20and%20IgG%20assay%20with%20conventional%20techniques%20for%20the%20diagnosis%20and%20follow-up%20of%20plasma%20cell%20dyscrasia&rft.jtitle=Annals%20of%20clinical%20biochemistry&rft.au=Paolini,%20Lucia&rft.date=2015-05-01&rft.volume=52&rft.issue=3&rft.spage=337&rft.epage=345&rft.pages=337-345&rft.issn=0004-5632&rft.eissn=1758-1001&rft_id=info:doi/10.1177/0004563214564225&rft_dat=%3Cproquest_cross%3E1674687142%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1674687142&rft_id=info:pmid/25468997&rft_sage_id=10.1177_0004563214564225&rfr_iscdi=true |