Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines
Objectives Plumbagin (PL), a naturally occurring quinoid, exerts antitumoral effects in diverse types of cancer cells. However, the effect of PL on tumor cell proliferation in oral squamous cell carcinoma (OSCC) remains poorly understood. In this study, we assessed the efficacy of PL, in human OSCC...
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| Veröffentlicht in: | Oral diseases 2015-05, Vol.21 (4), p.501-511 |
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| creator | Ono, T Ota, A Ito, K Nakaoka, T Karnan, S Konishi, H Furuhashi, A Hayashi, T Yamada, Y Hosokawa, Y Kazaoka, Y |
| description | Objectives
Plumbagin (PL), a naturally occurring quinoid, exerts antitumoral effects in diverse types of cancer cells. However, the effect of PL on tumor cell proliferation in oral squamous cell carcinoma (OSCC) remains poorly understood. In this study, we assessed the efficacy of PL, in human OSCC cells.
Methods
The effect of PL on the cell growth and apoptosis of OSCC cell lines was evaluated using MTT and Annexin V assays, respectively. The effect of PL on mitochondrial membrane potential loss and reactive oxygen species (ROS) generation was evaluated using flow cytometry analysis.
Results
MTT assay showed that PL dose‐dependently suppressed OSCC cell growth, with IC50 values ranging from 3.87 to 14.6 μM. Flow cytometry analysis revealed that PL treatment resulted in a significant decrease in mitochondrial membrane potential and an increase in the number of apoptotic cells. Notably, ROS generation was significantly elevated after PL treatment. Furthermore, a ROS scavenger, N‐acetylcysteine (NAC), clearly suppressed the decrease in mitochondrial membrane potential, increase of caspase‐3/7 activity, and apoptosis after PL treatment.
Conclusion
This study provides the considerable evidence of the tumor‐suppressive effect of PL, thereby highlighting its therapeutic potential for OSCC treatment. |
| doi_str_mv | 10.1111/odi.12310 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1674203727</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1674203727</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5270-b70d0174dfc28d3c5f5e0e07212ba71dcf114c04cf6ff4a877ca1eaf9ea09d213</originalsourceid><addsrcrecordid>eNp1kM1OwzAQhC0EglI48AIoEhc4BPwTx8kRChSkioIAtTfLcWwIJHFqJyp9e1wCHJDYy-5qvx2NBoADBE-RrzOTF6cIEwQ3wADFEIUwwXTTz4RGIcVkvgN2nXuDELGU4G2wgylNYJqyAXi4L7sqEy9FHbiuaaxyTrmg7SpjA6nKMnixZtm-Bv5urCgDt-hEZTrXH6WwsqhNJfq1LGrl9sCWFqVT-999CJ6vr55GN-FkOr4dnU9CSTGDYcZg7v1EuZY4yYmkmiqoIMMIZ4KhXGqEIgkjqWOtI5EwJgVSQqdKwDTHiAzBca_bWLPolGt5Vbi1DVErb5CjmEUYEoaZR4_-oG-ms7V3t6ZwEpMYU0-d9JS0xjmrNG9sUQm74gjydc7c58y_cvbs4bdil1Uq_yV_gvXAWQ8si1Kt_lfi08vbH8mw_yhcqz5-P4R95zEjjPLZ3ZjjRzqaTy5mPCWfhcSWTg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1672863625</pqid></control><display><type>article</type><title>Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ono, T ; Ota, A ; Ito, K ; Nakaoka, T ; Karnan, S ; Konishi, H ; Furuhashi, A ; Hayashi, T ; Yamada, Y ; Hosokawa, Y ; Kazaoka, Y</creator><creatorcontrib>Ono, T ; Ota, A ; Ito, K ; Nakaoka, T ; Karnan, S ; Konishi, H ; Furuhashi, A ; Hayashi, T ; Yamada, Y ; Hosokawa, Y ; Kazaoka, Y</creatorcontrib><description>Objectives
Plumbagin (PL), a naturally occurring quinoid, exerts antitumoral effects in diverse types of cancer cells. However, the effect of PL on tumor cell proliferation in oral squamous cell carcinoma (OSCC) remains poorly understood. In this study, we assessed the efficacy of PL, in human OSCC cells.
Methods
The effect of PL on the cell growth and apoptosis of OSCC cell lines was evaluated using MTT and Annexin V assays, respectively. The effect of PL on mitochondrial membrane potential loss and reactive oxygen species (ROS) generation was evaluated using flow cytometry analysis.
Results
MTT assay showed that PL dose‐dependently suppressed OSCC cell growth, with IC50 values ranging from 3.87 to 14.6 μM. Flow cytometry analysis revealed that PL treatment resulted in a significant decrease in mitochondrial membrane potential and an increase in the number of apoptotic cells. Notably, ROS generation was significantly elevated after PL treatment. Furthermore, a ROS scavenger, N‐acetylcysteine (NAC), clearly suppressed the decrease in mitochondrial membrane potential, increase of caspase‐3/7 activity, and apoptosis after PL treatment.
Conclusion
This study provides the considerable evidence of the tumor‐suppressive effect of PL, thereby highlighting its therapeutic potential for OSCC treatment.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.12310</identifier><identifier>PMID: 25580997</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>anti-cancer drug ; Antineoplastic Agents, Phytogenic - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Carcinoma, Squamous Cell - drug therapy ; Carcinoma, Squamous Cell - pathology ; Cell Cycle - drug effects ; Cell growth ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Chemotherapy ; Dentistry ; Flow cytometry ; Humans ; Mouth Neoplasms - drug therapy ; Mouth Neoplasms - pathology ; Naphthoquinones - pharmacology ; Oral cancer ; oral squamous cell carcinoma ; plumbagin ; reactive oxygen species</subject><ispartof>Oral diseases, 2015-05, Vol.21 (4), p.501-511</ispartof><rights>2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5270-b70d0174dfc28d3c5f5e0e07212ba71dcf114c04cf6ff4a877ca1eaf9ea09d213</citedby><cites>FETCH-LOGICAL-c5270-b70d0174dfc28d3c5f5e0e07212ba71dcf114c04cf6ff4a877ca1eaf9ea09d213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.12310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.12310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25580997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ono, T</creatorcontrib><creatorcontrib>Ota, A</creatorcontrib><creatorcontrib>Ito, K</creatorcontrib><creatorcontrib>Nakaoka, T</creatorcontrib><creatorcontrib>Karnan, S</creatorcontrib><creatorcontrib>Konishi, H</creatorcontrib><creatorcontrib>Furuhashi, A</creatorcontrib><creatorcontrib>Hayashi, T</creatorcontrib><creatorcontrib>Yamada, Y</creatorcontrib><creatorcontrib>Hosokawa, Y</creatorcontrib><creatorcontrib>Kazaoka, Y</creatorcontrib><title>Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
Plumbagin (PL), a naturally occurring quinoid, exerts antitumoral effects in diverse types of cancer cells. However, the effect of PL on tumor cell proliferation in oral squamous cell carcinoma (OSCC) remains poorly understood. In this study, we assessed the efficacy of PL, in human OSCC cells.
Methods
The effect of PL on the cell growth and apoptosis of OSCC cell lines was evaluated using MTT and Annexin V assays, respectively. The effect of PL on mitochondrial membrane potential loss and reactive oxygen species (ROS) generation was evaluated using flow cytometry analysis.
Results
MTT assay showed that PL dose‐dependently suppressed OSCC cell growth, with IC50 values ranging from 3.87 to 14.6 μM. Flow cytometry analysis revealed that PL treatment resulted in a significant decrease in mitochondrial membrane potential and an increase in the number of apoptotic cells. Notably, ROS generation was significantly elevated after PL treatment. Furthermore, a ROS scavenger, N‐acetylcysteine (NAC), clearly suppressed the decrease in mitochondrial membrane potential, increase of caspase‐3/7 activity, and apoptosis after PL treatment.
Conclusion
This study provides the considerable evidence of the tumor‐suppressive effect of PL, thereby highlighting its therapeutic potential for OSCC treatment.</description><subject>anti-cancer drug</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Carcinoma, Squamous Cell - drug therapy</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Cycle - drug effects</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Chemotherapy</subject><subject>Dentistry</subject><subject>Flow cytometry</subject><subject>Humans</subject><subject>Mouth Neoplasms - drug therapy</subject><subject>Mouth Neoplasms - pathology</subject><subject>Naphthoquinones - pharmacology</subject><subject>Oral cancer</subject><subject>oral squamous cell carcinoma</subject><subject>plumbagin</subject><subject>reactive oxygen species</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1OwzAQhC0EglI48AIoEhc4BPwTx8kRChSkioIAtTfLcWwIJHFqJyp9e1wCHJDYy-5qvx2NBoADBE-RrzOTF6cIEwQ3wADFEIUwwXTTz4RGIcVkvgN2nXuDELGU4G2wgylNYJqyAXi4L7sqEy9FHbiuaaxyTrmg7SpjA6nKMnixZtm-Bv5urCgDt-hEZTrXH6WwsqhNJfq1LGrl9sCWFqVT-999CJ6vr55GN-FkOr4dnU9CSTGDYcZg7v1EuZY4yYmkmiqoIMMIZ4KhXGqEIgkjqWOtI5EwJgVSQqdKwDTHiAzBca_bWLPolGt5Vbi1DVErb5CjmEUYEoaZR4_-oG-ms7V3t6ZwEpMYU0-d9JS0xjmrNG9sUQm74gjydc7c58y_cvbs4bdil1Uq_yV_gvXAWQ8si1Kt_lfi08vbH8mw_yhcqz5-P4R95zEjjPLZ3ZjjRzqaTy5mPCWfhcSWTg</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Ono, T</creator><creator>Ota, A</creator><creator>Ito, K</creator><creator>Nakaoka, T</creator><creator>Karnan, S</creator><creator>Konishi, H</creator><creator>Furuhashi, A</creator><creator>Hayashi, T</creator><creator>Yamada, Y</creator><creator>Hosokawa, Y</creator><creator>Kazaoka, Y</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201505</creationdate><title>Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines</title><author>Ono, T ; Ota, A ; Ito, K ; Nakaoka, T ; Karnan, S ; Konishi, H ; Furuhashi, A ; Hayashi, T ; Yamada, Y ; Hosokawa, Y ; Kazaoka, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5270-b70d0174dfc28d3c5f5e0e07212ba71dcf114c04cf6ff4a877ca1eaf9ea09d213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>anti-cancer drug</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Carcinoma, Squamous Cell - drug therapy</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Cycle - drug effects</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Chemotherapy</topic><topic>Dentistry</topic><topic>Flow cytometry</topic><topic>Humans</topic><topic>Mouth Neoplasms - drug therapy</topic><topic>Mouth Neoplasms - pathology</topic><topic>Naphthoquinones - pharmacology</topic><topic>Oral cancer</topic><topic>oral squamous cell carcinoma</topic><topic>plumbagin</topic><topic>reactive oxygen species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ono, T</creatorcontrib><creatorcontrib>Ota, A</creatorcontrib><creatorcontrib>Ito, K</creatorcontrib><creatorcontrib>Nakaoka, T</creatorcontrib><creatorcontrib>Karnan, S</creatorcontrib><creatorcontrib>Konishi, H</creatorcontrib><creatorcontrib>Furuhashi, A</creatorcontrib><creatorcontrib>Hayashi, T</creatorcontrib><creatorcontrib>Yamada, Y</creatorcontrib><creatorcontrib>Hosokawa, Y</creatorcontrib><creatorcontrib>Kazaoka, Y</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ono, T</au><au>Ota, A</au><au>Ito, K</au><au>Nakaoka, T</au><au>Karnan, S</au><au>Konishi, H</au><au>Furuhashi, A</au><au>Hayashi, T</au><au>Yamada, Y</au><au>Hosokawa, Y</au><au>Kazaoka, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2015-05</date><risdate>2015</risdate><volume>21</volume><issue>4</issue><spage>501</spage><epage>511</epage><pages>501-511</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
Plumbagin (PL), a naturally occurring quinoid, exerts antitumoral effects in diverse types of cancer cells. However, the effect of PL on tumor cell proliferation in oral squamous cell carcinoma (OSCC) remains poorly understood. In this study, we assessed the efficacy of PL, in human OSCC cells.
Methods
The effect of PL on the cell growth and apoptosis of OSCC cell lines was evaluated using MTT and Annexin V assays, respectively. The effect of PL on mitochondrial membrane potential loss and reactive oxygen species (ROS) generation was evaluated using flow cytometry analysis.
Results
MTT assay showed that PL dose‐dependently suppressed OSCC cell growth, with IC50 values ranging from 3.87 to 14.6 μM. Flow cytometry analysis revealed that PL treatment resulted in a significant decrease in mitochondrial membrane potential and an increase in the number of apoptotic cells. Notably, ROS generation was significantly elevated after PL treatment. Furthermore, a ROS scavenger, N‐acetylcysteine (NAC), clearly suppressed the decrease in mitochondrial membrane potential, increase of caspase‐3/7 activity, and apoptosis after PL treatment.
Conclusion
This study provides the considerable evidence of the tumor‐suppressive effect of PL, thereby highlighting its therapeutic potential for OSCC treatment.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>25580997</pmid><doi>10.1111/odi.12310</doi><tpages>11</tpages></addata></record> |
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| subjects | anti-cancer drug Antineoplastic Agents, Phytogenic - pharmacology Apoptosis Apoptosis - drug effects Carcinoma, Squamous Cell - drug therapy Carcinoma, Squamous Cell - pathology Cell Cycle - drug effects Cell growth Cell Line, Tumor Cell Proliferation - drug effects Chemotherapy Dentistry Flow cytometry Humans Mouth Neoplasms - drug therapy Mouth Neoplasms - pathology Naphthoquinones - pharmacology Oral cancer oral squamous cell carcinoma plumbagin reactive oxygen species |
| title | Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines |
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