Expression and function of fatty acid-binding protein 4 in epithelial cell of uterine endometrium
The aims of this study were to delineate the expression of fatty‐acid binding protein (FABP) 4 in human uterine endometrium and its function in the regulation of proliferation, migration and invasion of epithelial cells. Immunohistochenistry, immunofluorence and Western blotting were used to determi...
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| Veröffentlicht in: | Cell biology international 2015-05, Vol.39 (5), p.540-547 |
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| creator | Zhu, Qiuyuan Jin, Yan Wang, Peng Wang, Hanzhi Lu, Bingjian Wang, Zhengping Dong, Minyue |
| description | The aims of this study were to delineate the expression of fatty‐acid binding protein (FABP) 4 in human uterine endometrium and its function in the regulation of proliferation, migration and invasion of epithelial cells. Immunohistochenistry, immunofluorence and Western blotting were used to determine the expression and cellular localization of FABP4 in endometrium and endometrial epithelial cell lines. Interference of small ribonuclear acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4. The proliferation, migration and invasion of epithelial cells were evaluated with CCK‐8 assay, wound‐healing test and transwell analysis respectively. We found that FABP4 was expressed by epithelial cells of proliferative endometrium and epithelial and stromal cells of secrectory endometrium. Epithelial cell lines Ishikawa and RL‐952 expressed FABP4 and this expression was decreased by FABP4 siRNA. FABP4 siRNA and specific FABP4 inhibition significantly decreased the proliferation, migration and invasion of epithelial cell lines. We concluded that FABP4 is functionally expressed in endometrial epithelium and is necessary for maintaining the cell function of epithelial cells of endometrium. |
| doi_str_mv | 10.1002/cbin.10429 |
| format | Article |
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Immunohistochenistry, immunofluorence and Western blotting were used to determine the expression and cellular localization of FABP4 in endometrium and endometrial epithelial cell lines. Interference of small ribonuclear acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4. The proliferation, migration and invasion of epithelial cells were evaluated with CCK‐8 assay, wound‐healing test and transwell analysis respectively. We found that FABP4 was expressed by epithelial cells of proliferative endometrium and epithelial and stromal cells of secrectory endometrium. Epithelial cell lines Ishikawa and RL‐952 expressed FABP4 and this expression was decreased by FABP4 siRNA. FABP4 siRNA and specific FABP4 inhibition significantly decreased the proliferation, migration and invasion of epithelial cell lines. We concluded that FABP4 is functionally expressed in endometrial epithelium and is necessary for maintaining the cell function of epithelial cells of endometrium.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1002/cbin.10429</identifier><identifier>PMID: 25572488</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>biology ; Cell Movement - drug effects ; Cell Movement - genetics ; Cell Proliferation - drug effects ; Cell Proliferation - genetics ; Cells, Cultured ; endometrial epithelial cell ; Endometrium - drug effects ; Endometrium - metabolism ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; fatty acid-binding protein 4 ; Fatty Acid-Binding Proteins - antagonists & inhibitors ; Fatty Acid-Binding Proteins - genetics ; Fatty Acid-Binding Proteins - physiology ; Female ; function ; Humans ; maintenance ; Medical research ; Migration ; proliferation ; RNA, Small Interfering - pharmacology</subject><ispartof>Cell biology international, 2015-05, Vol.39 (5), p.540-547</ispartof><rights>2015 International Federation for Cell Biology</rights><rights>2015 International Federation for Cell Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4619-3199bd86084304fa4db7e916d44d63355029a7cdae5838408212a19ec250f87a3</citedby><cites>FETCH-LOGICAL-c4619-3199bd86084304fa4db7e916d44d63355029a7cdae5838408212a19ec250f87a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbin.10429$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbin.10429$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25572488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Qiuyuan</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Wang, Peng</creatorcontrib><creatorcontrib>Wang, Hanzhi</creatorcontrib><creatorcontrib>Lu, Bingjian</creatorcontrib><creatorcontrib>Wang, Zhengping</creatorcontrib><creatorcontrib>Dong, Minyue</creatorcontrib><title>Expression and function of fatty acid-binding protein 4 in epithelial cell of uterine endometrium</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>The aims of this study were to delineate the expression of fatty‐acid binding protein (FABP) 4 in human uterine endometrium and its function in the regulation of proliferation, migration and invasion of epithelial cells. Immunohistochenistry, immunofluorence and Western blotting were used to determine the expression and cellular localization of FABP4 in endometrium and endometrial epithelial cell lines. Interference of small ribonuclear acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4. The proliferation, migration and invasion of epithelial cells were evaluated with CCK‐8 assay, wound‐healing test and transwell analysis respectively. We found that FABP4 was expressed by epithelial cells of proliferative endometrium and epithelial and stromal cells of secrectory endometrium. Epithelial cell lines Ishikawa and RL‐952 expressed FABP4 and this expression was decreased by FABP4 siRNA. FABP4 siRNA and specific FABP4 inhibition significantly decreased the proliferation, migration and invasion of epithelial cell lines. We concluded that FABP4 is functionally expressed in endometrial epithelium and is necessary for maintaining the cell function of epithelial cells of endometrium.</description><subject>biology</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Proliferation - genetics</subject><subject>Cells, Cultured</subject><subject>endometrial epithelial cell</subject><subject>Endometrium - drug effects</subject><subject>Endometrium - metabolism</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>fatty acid-binding protein 4</subject><subject>Fatty Acid-Binding Proteins - antagonists & inhibitors</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Fatty Acid-Binding Proteins - physiology</subject><subject>Female</subject><subject>function</subject><subject>Humans</subject><subject>maintenance</subject><subject>Medical research</subject><subject>Migration</subject><subject>proliferation</subject><subject>RNA, Small Interfering - pharmacology</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAUhS0EoqWw4QcgS2wQUqjfsZcwakurtix4io3liW_AJXEG2xGdf4_Tabtgwca-lr5z7vFB6Dklbygh7LBbh1gnwcwDtE-JkY3mUj5cZiUbZYzcQ09yviKEUqHVY7THpGyZ0HofuaPrTYKcwxSxix73c-zK8ph63LtStth1wTd1gw_xB96kqUCIWOB6wCaUnzAEN-AOhmGRzAVSiIAh-mmEksI8PkWPejdkeHZ7H6DPx0efVu-b8w8np6u3500nFDUNp8asvVZEC05E74Rft2Co8kJ4xet_CDOu7bwDqbkWRDPKHDXQMUl63Tp-gF7tfGvG3zPkYseQl1wuwjRnS1UrWDWhvKIv_0GvpjnFmm6huKr-SlXq9Y7q0pRzgt5uUhhd2lpK7FK8XYq3N8VX-MWt5bwewd-jd01XgO6AP2GA7X-s7Ord6eWdabPThFzg-l7j0i9bY7bSfr08sccX3799_MLPrOR_AaxLm64</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Zhu, Qiuyuan</creator><creator>Jin, Yan</creator><creator>Wang, Peng</creator><creator>Wang, Hanzhi</creator><creator>Lu, Bingjian</creator><creator>Wang, Zhengping</creator><creator>Dong, Minyue</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201505</creationdate><title>Expression and function of fatty acid-binding protein 4 in epithelial cell of uterine endometrium</title><author>Zhu, Qiuyuan ; Jin, Yan ; Wang, Peng ; Wang, Hanzhi ; Lu, Bingjian ; Wang, Zhengping ; Dong, Minyue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4619-3199bd86084304fa4db7e916d44d63355029a7cdae5838408212a19ec250f87a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>biology</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Proliferation - genetics</topic><topic>Cells, Cultured</topic><topic>endometrial epithelial cell</topic><topic>Endometrium - drug effects</topic><topic>Endometrium - metabolism</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>fatty acid-binding protein 4</topic><topic>Fatty Acid-Binding Proteins - antagonists & inhibitors</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Fatty Acid-Binding Proteins - physiology</topic><topic>Female</topic><topic>function</topic><topic>Humans</topic><topic>maintenance</topic><topic>Medical research</topic><topic>Migration</topic><topic>proliferation</topic><topic>RNA, Small Interfering - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Qiuyuan</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Wang, Peng</creatorcontrib><creatorcontrib>Wang, Hanzhi</creatorcontrib><creatorcontrib>Lu, Bingjian</creatorcontrib><creatorcontrib>Wang, Zhengping</creatorcontrib><creatorcontrib>Dong, Minyue</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Qiuyuan</au><au>Jin, Yan</au><au>Wang, Peng</au><au>Wang, Hanzhi</au><au>Lu, Bingjian</au><au>Wang, Zhengping</au><au>Dong, Minyue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and function of fatty acid-binding protein 4 in epithelial cell of uterine endometrium</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2015-05</date><risdate>2015</risdate><volume>39</volume><issue>5</issue><spage>540</spage><epage>547</epage><pages>540-547</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>The aims of this study were to delineate the expression of fatty‐acid binding protein (FABP) 4 in human uterine endometrium and its function in the regulation of proliferation, migration and invasion of epithelial cells. Immunohistochenistry, immunofluorence and Western blotting were used to determine the expression and cellular localization of FABP4 in endometrium and endometrial epithelial cell lines. Interference of small ribonuclear acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4. The proliferation, migration and invasion of epithelial cells were evaluated with CCK‐8 assay, wound‐healing test and transwell analysis respectively. We found that FABP4 was expressed by epithelial cells of proliferative endometrium and epithelial and stromal cells of secrectory endometrium. Epithelial cell lines Ishikawa and RL‐952 expressed FABP4 and this expression was decreased by FABP4 siRNA. FABP4 siRNA and specific FABP4 inhibition significantly decreased the proliferation, migration and invasion of epithelial cell lines. We concluded that FABP4 is functionally expressed in endometrial epithelium and is necessary for maintaining the cell function of epithelial cells of endometrium.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>25572488</pmid><doi>10.1002/cbin.10429</doi><tpages>8</tpages></addata></record> |
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| subjects | biology Cell Movement - drug effects Cell Movement - genetics Cell Proliferation - drug effects Cell Proliferation - genetics Cells, Cultured endometrial epithelial cell Endometrium - drug effects Endometrium - metabolism Epithelial Cells - drug effects Epithelial Cells - metabolism fatty acid-binding protein 4 Fatty Acid-Binding Proteins - antagonists & inhibitors Fatty Acid-Binding Proteins - genetics Fatty Acid-Binding Proteins - physiology Female function Humans maintenance Medical research Migration proliferation RNA, Small Interfering - pharmacology |
| title | Expression and function of fatty acid-binding protein 4 in epithelial cell of uterine endometrium |
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