Molecular Cloning and Construction of the Coding Region for Human Acetylcholinesterase Reveals a G + C-Rich Attenuating Structure

To study the primary structure of human acetylcholinesterase (AcChoEase; EC 3.1.1.7) and its gene expression and amplification, cDNA libraries from human tissues expressing oocyte-translatable AcChoEase mRNA were constructed and screened with labeled oligodeoxynucleotide probes. Several cDNA clones...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1990-12, Vol.87 (24), p.9688-9692
Hauptverfasser:	Soreq, Hermona, Ben-Aziz, Revital, Prody, Catherine A., Seidman, Shlomo, Gnatt, Averell, Neville, Lewis, Lieman-Hurwitz, Judith, lev-Lehman, Efrat, Ginzberg, Dalia, Lapidot-Lifson, Yaron, Zakut, Haim
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ACETYLCHOLINE acetylcholinesterase Acetylcholinesterase - genetics Acetylcholinesterase - metabolism AMINES AMINO ACID SEQUENCE Amino acids AMMONIUM COMPOUNDS ANIMALS AUTONOMIC NERVOUS SYSTEM AGENTS Base Sequence BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences CARBOXYLESTERASES cDNA cholinesterase CLONING Cloning, Molecular Complementary DNA Cytosine DAYS LIVING RADIOISOTOPES DNA DNA HYBRIDIZATION DNA probes DNA SEQUENCING DNA-CLONING DRUGS ENZYMES ESTERASES ESTERS Female Fundamental and applied biological sciences. Psychology GENE AMPLIFICATION Gene expression Gene Library Genomics Guanine Humans HYBRIDIZATION HYDROLASES ISOTOPES LIGHT NUCLEI MAMMALS MAN MESSENGER-RNA Models, Molecular Molecular and cellular biology Molecular genetics Molecular Sequence Data MOLECULAR STRUCTURE NEUROREGULATORS NUCLEI Nucleic Acid Conformation NUCLEIC ACIDS Nucleotides ODD-ODD NUCLEI Oligonucleotide Probes OLIGONUCLEOTIDES Oocytes Oocytes - enzymology ORGANIC COMPOUNDS PARASYMPATHOMIMETICS PHOSPHORUS 32 PHOSPHORUS ISOTOPES PRIMATES Protein Biosynthesis QUATERNARY COMPOUNDS RADIOISOTOPES RECOMBINANT DNA restriction fragment length polymorphism Restriction Mapping RNA RNA, Messenger - genetics Sequence Homology, Nucleic Acid Sequencing STRUCTURAL CHEMICAL ANALYSIS Torpedo Transcriptional regulatory elements VERTEBRATES Xenopus
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