The characterization of ion channels formed by Pasteurella multocida dermonecrotic toxin
The influence of the dermonecrotic lethal toxin (approximately 120 kDa) produced by Pasteurella multocida serovarian D on planar phospholipid bilayers was studied. It was found that the toxin is able to increase the conductance of the bilayers by formation of low-conductive and cation-selective ion...
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| Veröffentlicht in: | Medical microbiology and immunology 1994-11, Vol.183 (5), p.229-237 |
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| container_title | Medical microbiology and immunology |
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| creator | KRASILNIKOV, O. V TERNOVSKY, V. I NAVASARDYANTS, D. G KALMYKOVA, L. I |
| description | The influence of the dermonecrotic lethal toxin (approximately 120 kDa) produced by Pasteurella multocida serovarian D on planar phospholipid bilayers was studied. It was found that the toxin is able to increase the conductance of the bilayers by formation of low-conductive and cation-selective ion channels [27 pS at 4.0 M KCl, pH 7.5; zero current potential equals to -14.5 +/- 0.5 mV at threefold transmembrane gradient KCl (120 mM/40 mM)]. In biionic conditions the channels displayed weak selectivity between Na, K and Ca ions. The shapes of current-voltage characteristics (which were measured at different pH and salt concentrations) indicate that an energetic barrier for passing ions is situated near the center of the water pore of the ion channels. The effective diameter of the ion channel's water pore was established to be equal to 2.1 +/- 0.3 nm. |
| doi_str_mv | 10.1007/BF00198457 |
| format | Article |
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The shapes of current-voltage characteristics (which were measured at different pH and salt concentrations) indicate that an energetic barrier for passing ions is situated near the center of the water pore of the ion channels. The effective diameter of the ion channel's water pore was established to be equal to 2.1 +/- 0.3 nm.</description><subject>Bacterial Toxins - pharmacology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Dermotoxins - pharmacology</subject><subject>Electric Conductivity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - metabolism</subject><subject>Lipid Bilayers - metabolism</subject><subject>Membranes, Artificial</subject><subject>Microbiology</subject><subject>Pasteurella - chemistry</subject><subject>Pasteurella multocida</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Phospholipids - metabolism</subject><issn>0300-8584</issn><issn>1432-1831</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFLwzAYxYMoc04v3oUcxINQ_dK0SXrU4VQY6GGCt5KmX1mkbWbSgvOvt2Nlnh5878fjfY-QSwZ3DEDePy4AWKaSVB6RKUt4HDHF2TGZAgeIVKqSU3IWwtdASRHDhExkykWc8Sn5XK2RmrX22nTo7a_urGupq-hOhnvbYh1o5XyDJS229F2HDnuPda1p09edM7bUtETfuBaNd501tHM_tj0nJ5WuA16MOiMfi6fV_CVavj2_zh-WkeFJ3EWFAYFCcmbUUDlOlZYJSJ3p0hSJVMgNFCyTVYZYCg5CmapgskhZIooyBuAzcrPP3Xj33WPo8sYGs-vXoutDzoRMGAyjzMjtHhxahuCxyjfeNtpvcwb5bsb8f8YBvhpT-2L4_ICOuw3-9ejrYHRded0aGw4Y52maMcH_AKR0ed0</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>KRASILNIKOV, O. 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I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The characterization of ion channels formed by Pasteurella multocida dermonecrotic toxin</atitle><jtitle>Medical microbiology and immunology</jtitle><addtitle>Med Microbiol Immunol</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>183</volume><issue>5</issue><spage>229</spage><epage>237</epage><pages>229-237</pages><issn>0300-8584</issn><eissn>1432-1831</eissn><coden>MMIYAO</coden><abstract>The influence of the dermonecrotic lethal toxin (approximately 120 kDa) produced by Pasteurella multocida serovarian D on planar phospholipid bilayers was studied. It was found that the toxin is able to increase the conductance of the bilayers by formation of low-conductive and cation-selective ion channels [27 pS at 4.0 M KCl, pH 7.5; zero current potential equals to -14.5 +/- 0.5 mV at threefold transmembrane gradient KCl (120 mM/40 mM)]. In biionic conditions the channels displayed weak selectivity between Na, K and Ca ions. The shapes of current-voltage characteristics (which were measured at different pH and salt concentrations) indicate that an energetic barrier for passing ions is situated near the center of the water pore of the ion channels. The effective diameter of the ion channel's water pore was established to be equal to 2.1 +/- 0.3 nm.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>7536293</pmid><doi>10.1007/BF00198457</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0300-8584 |
| ispartof | Medical microbiology and immunology, 1994-11, Vol.183 (5), p.229-237 |
| issn | 0300-8584 1432-1831 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Bacterial Toxins - pharmacology Bacteriology Biological and medical sciences Cell Membrane Permeability - drug effects Dermotoxins - pharmacology Electric Conductivity Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Ion Channels - drug effects Ion Channels - metabolism Lipid Bilayers - metabolism Membranes, Artificial Microbiology Pasteurella - chemistry Pasteurella multocida Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phospholipids - metabolism |
| title | The characterization of ion channels formed by Pasteurella multocida dermonecrotic toxin |
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