Effects of hirudin on the expression of basic fibroblast growth factor and transforming growth factor-β1 in human gingival fibroblasts
This study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func- tion in the mechanism of gingival remodeling. After culturing was perf...
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| Veröffentlicht in: | Hua xi kou qiang yi xue za zhi 2015-02, Vol.33 (1), p.6-10 |
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| container_title | Hua xi kou qiang yi xue za zhi |
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| creator | Yi, Zheng Kun, Xuan Lan, Nan Shuixue, Mo |
| description | This study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func- tion in the mechanism of gingival remodeling.
After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry.
Compared with normal HGFs, TGF-β1 expression promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P < 0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P < 0.05).
Orthodontic force may influence the balance of collagen synthesis and d |
| format | Article |
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After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry.
Compared with normal HGFs, TGF-β1 expression promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P < 0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P < 0.05).
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After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry.
Compared with normal HGFs, TGF-β1 expression promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P < 0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P < 0.05).
Orthodontic force may influence the balance of collagen synthesis and d</description><subject>Dentistry</subject><subject>Fibroblast Growth Factor 2</subject><subject>Fibroblasts</subject><subject>Gingiva</subject><subject>Hirudins</subject><subject>Humans</subject><subject>RNA, Messenger</subject><subject>Transforming Growth Factor beta</subject><subject>Transforming Growth Factor beta1</subject><issn>1000-1182</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtOwzAURTMA0ap0C8hDJpH8qeN6iKrykZCYwDjy7zWWEjvYDp8VsB8WwpqIRJFg9HR17j2Dd1ItCca4JmRLF9U6Z68xJ0zgjWRn1YLyraBU4mX1sQdwpmQUAXU-TdYHFAMqnUPubUxuXs5xhlplbxB4naLuVS7okOJr6RAoU2JCKlhUkgoZYhp8OPzH9dcnQbO5mwYV0GHm_kX1f2z5vDoF1We3Pt5V9XS9f9zd1vcPN3e7q_t6JLQpNQfHwRDNMW2U0JwCUIM3jBojFRC3sQZzDcZY4xiWkkpFLSNYOhDQWMdW1eWPd0zxeXK5tIPPxvW9Ci5OuSWNYEIyLthcvThWJz04247JDyq9t7_PY9-bU2-p</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Yi, Zheng</creator><creator>Kun, Xuan</creator><creator>Lan, Nan</creator><creator>Shuixue, Mo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201502</creationdate><title>Effects of hirudin on the expression of basic fibroblast growth factor and transforming growth factor-β1 in human gingival fibroblasts</title><author>Yi, Zheng ; Kun, Xuan ; Lan, Nan ; Shuixue, Mo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-5fe5fc1b5026a7b52ff2c0432cc9af1e4dc05bfccdce309929a2d3109ef7f6de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2015</creationdate><topic>Dentistry</topic><topic>Fibroblast Growth Factor 2</topic><topic>Fibroblasts</topic><topic>Gingiva</topic><topic>Hirudins</topic><topic>Humans</topic><topic>RNA, Messenger</topic><topic>Transforming Growth Factor beta</topic><topic>Transforming Growth Factor beta1</topic><toplevel>online_resources</toplevel><creatorcontrib>Yi, Zheng</creatorcontrib><creatorcontrib>Kun, Xuan</creatorcontrib><creatorcontrib>Lan, Nan</creatorcontrib><creatorcontrib>Shuixue, Mo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Hua xi kou qiang yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, Zheng</au><au>Kun, Xuan</au><au>Lan, Nan</au><au>Shuixue, Mo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of hirudin on the expression of basic fibroblast growth factor and transforming growth factor-β1 in human gingival fibroblasts</atitle><jtitle>Hua xi kou qiang yi xue za zhi</jtitle><addtitle>Hua Xi Kou Qiang Yi Xue Za Zhi</addtitle><date>2015-02</date><risdate>2015</risdate><volume>33</volume><issue>1</issue><spage>6</spage><epage>10</epage><pages>6-10</pages><issn>1000-1182</issn><abstract>This study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func- tion in the mechanism of gingival remodeling.
After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry.
Compared with normal HGFs, TGF-β1 expression promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P < 0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P < 0.05).
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| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Dentistry Fibroblast Growth Factor 2 Fibroblasts Gingiva Hirudins Humans RNA, Messenger Transforming Growth Factor beta Transforming Growth Factor beta1 |
| title | Effects of hirudin on the expression of basic fibroblast growth factor and transforming growth factor-β1 in human gingival fibroblasts |
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