Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test
On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the h...
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| Veröffentlicht in: | Journal of Applied Bacteriology 1995-03, Vol.78 (3), p.316-326 |
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| container_title | Journal of Applied Bacteriology |
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| creator | Le Jeune, C Lonvaud-Funel, A Brink, B. ten Hofstra, H Vossen, J.M.B.M. van der |
| description | On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1/JV17HC were also useful for the detection of histamine-forming Leuconostoc oenos strains in PCR. The 150 base pair amplification product of the decarboxylating Leuc. oenos strain generated with primer set CL1/CL2 was sequenced. Alignment studies showed a high degree of relatedness among the hdcA gene products of Gram-positive bacteria. The amplification products of the hdcA genes from Lact. buchneri and Leuc. oenos were used to serve as a DNA probe in hybridization studies. All histidine-decarboxylating lactic acid bacteria gave a hybridization signal with the DNA probes. In hybridization only one false-positive signal with a Lactobacillus lindneri strain was observed, which was anticipated to contain a truncated hdcA gene. In addition to these DNA probe tests, a simple and reliable activity test is presented, which can be used during starter selection to test strains for histidine decarboxylase activity. |
| doi_str_mv | 10.1111/j.1365-2672.1995.tb05032.x |
| format | Article |
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All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1/JV17HC were also useful for the detection of histamine-forming Leuconostoc oenos strains in PCR. The 150 base pair amplification product of the decarboxylating Leuc. oenos strain generated with primer set CL1/CL2 was sequenced. Alignment studies showed a high degree of relatedness among the hdcA gene products of Gram-positive bacteria. The amplification products of the hdcA genes from Lact. buchneri and Leuc. oenos were used to serve as a DNA probe in hybridization studies. All histidine-decarboxylating lactic acid bacteria gave a hybridization signal with the DNA probes. In hybridization only one false-positive signal with a Lactobacillus lindneri strain was observed, which was anticipated to contain a truncated hdcA gene. In addition to these DNA probe tests, a simple and reliable activity test is presented, which can be used during starter selection to test strains for histidine decarboxylase activity.</description><identifier>ISSN: 0021-8847</identifier><identifier>EISSN: 2056-5232</identifier><identifier>DOI: 10.1111/j.1365-2672.1995.tb05032.x</identifier><identifier>PMID: 7730207</identifier><identifier>CODEN: JABAA4</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; carboxy-lyases ; Clostridium perfringens - enzymology ; Clostridium perfringens - genetics ; Clostridium perfringens - isolation & purification ; comparisons ; detection ; DNA Probes ; enzyme activity ; Evaluation Studies as Topic ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; genbank/u09485 ; hdca gene ; histamine ; Histamine - metabolism ; histamine producing lactic acid bacteria ; histidine ; Histidine - metabolism ; histidine decarboxylase ; Histidine Decarboxylase - genetics ; Histidine Decarboxylase - metabolism ; histidine decarboxylation ; Lactates - metabolism ; Lactic Acid ; lactic acid bacteria ; Lactobacillus - enzymology ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Leuconostoc - enzymology ; Leuconostoc - genetics ; Microbiology ; Molecular Sequence Data ; nucleic acid hybridization ; nucleotide sequences ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; sequence alignment ; Sequence Homology, Nucleic Acid ; structural genes</subject><ispartof>Journal of Applied Bacteriology, 1995-03, Vol.78 (3), p.316-326</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5196-d87e329fce35aa055cf6dcbcf53550f0bc4fdcbff3cff9fbd898ee8b1c83456c3</citedby><cites>FETCH-LOGICAL-c5196-d87e329fce35aa055cf6dcbcf53550f0bc4fdcbff3cff9fbd898ee8b1c83456c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3458219$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7730207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Jeune, C</creatorcontrib><creatorcontrib>Lonvaud-Funel, A</creatorcontrib><creatorcontrib>Brink, B. ten</creatorcontrib><creatorcontrib>Hofstra, H</creatorcontrib><creatorcontrib>Vossen, J.M.B.M. van der</creatorcontrib><title>Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test</title><title>Journal of Applied Bacteriology</title><addtitle>J Appl Bacteriol</addtitle><description>On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1/JV17HC were also useful for the detection of histamine-forming Leuconostoc oenos strains in PCR. The 150 base pair amplification product of the decarboxylating Leuc. oenos strain generated with primer set CL1/CL2 was sequenced. Alignment studies showed a high degree of relatedness among the hdcA gene products of Gram-positive bacteria. The amplification products of the hdcA genes from Lact. buchneri and Leuc. oenos were used to serve as a DNA probe in hybridization studies. All histidine-decarboxylating lactic acid bacteria gave a hybridization signal with the DNA probes. In hybridization only one false-positive signal with a Lactobacillus lindneri strain was observed, which was anticipated to contain a truncated hdcA gene. In addition to these DNA probe tests, a simple and reliable activity test is presented, which can be used during starter selection to test strains for histidine decarboxylase activity.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>carboxy-lyases</subject><subject>Clostridium perfringens - enzymology</subject><subject>Clostridium perfringens - genetics</subject><subject>Clostridium perfringens - isolation & purification</subject><subject>comparisons</subject><subject>detection</subject><subject>DNA Probes</subject><subject>enzyme activity</subject><subject>Evaluation Studies as Topic</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/u09485</subject><subject>hdca gene</subject><subject>histamine</subject><subject>Histamine - metabolism</subject><subject>histamine producing lactic acid bacteria</subject><subject>histidine</subject><subject>Histidine - metabolism</subject><subject>histidine decarboxylase</subject><subject>Histidine Decarboxylase - genetics</subject><subject>Histidine Decarboxylase - metabolism</subject><subject>histidine decarboxylation</subject><subject>Lactates - metabolism</subject><subject>Lactic Acid</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus - enzymology</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - isolation & purification</subject><subject>Leuconostoc - enzymology</subject><subject>Leuconostoc - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>nucleic acid hybridization</subject><subject>nucleotide sequences</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>sequence alignment</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>structural genes</subject><issn>0021-8847</issn><issn>2056-5232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEmP1DAQhS0EGpqBn4CwEOJEgpd2ljkgtXpYNSwC5mzZTnlwK4kb2z10Tvx1HHU0d3xxleqr90oPoeeUlDS_17uS8koUrKpZSdtWlEkTQTgrj_fQihFRFYJxdh-tCGG0aJp1_RA9inGXW9Y25Ayd1TUnjNQr9PcSbqH3-wHGhL3FCneQwCTnRxynmGDA1gf8y8XkOjdCHhsVtD9OvUpuvMG9yrDByrgO61xDcCoXETqcJS6_bPA-eA3xFf62_Y7V2OF54dalCSeI6TF6YFUf4cnyn6Prd29_bj8UV1_ff9xurgojaFsVXVMDZ601wIVSRAhjq85oYwUXgliizdrm3lpurG2t7pq2AWg0NQ1fi8rwc_TypJuv-X3IxnJw0UDfqxH8IUpa1VxQwTN4cQJN8DEGsHIf3KDCJCmRc_pyJ-f05Zy-nNOXS_rymJefLi4HPUB3t7rEnecvlrmKRvU2qNG4eIflUxtG24y9OWF_XA_TfxwgP20-c1plgWcnAau8VDche1z_YIRyQgVZZ5b_A1zIrV8</recordid><startdate>199503</startdate><enddate>199503</enddate><creator>Le Jeune, C</creator><creator>Lonvaud-Funel, A</creator><creator>Brink, B. ten</creator><creator>Hofstra, H</creator><creator>Vossen, J.M.B.M. van der</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>199503</creationdate><title>Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test</title><author>Le Jeune, C ; Lonvaud-Funel, A ; Brink, B. ten ; Hofstra, H ; Vossen, J.M.B.M. van der</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5196-d87e329fce35aa055cf6dcbcf53550f0bc4fdcbff3cff9fbd898ee8b1c83456c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>carboxy-lyases</topic><topic>Clostridium perfringens - enzymology</topic><topic>Clostridium perfringens - genetics</topic><topic>Clostridium perfringens - isolation & purification</topic><topic>comparisons</topic><topic>detection</topic><topic>DNA Probes</topic><topic>enzyme activity</topic><topic>Evaluation Studies as Topic</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/u09485</topic><topic>hdca gene</topic><topic>histamine</topic><topic>Histamine - metabolism</topic><topic>histamine producing lactic acid bacteria</topic><topic>histidine</topic><topic>Histidine - metabolism</topic><topic>histidine decarboxylase</topic><topic>Histidine Decarboxylase - genetics</topic><topic>Histidine Decarboxylase - metabolism</topic><topic>histidine decarboxylation</topic><topic>Lactates - metabolism</topic><topic>Lactic Acid</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus - enzymology</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - isolation & purification</topic><topic>Leuconostoc - enzymology</topic><topic>Leuconostoc - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>nucleic acid hybridization</topic><topic>nucleotide sequences</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>sequence alignment</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le Jeune, C</creatorcontrib><creatorcontrib>Lonvaud-Funel, A</creatorcontrib><creatorcontrib>Brink, B. ten</creatorcontrib><creatorcontrib>Hofstra, H</creatorcontrib><creatorcontrib>Vossen, J.M.B.M. van der</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of Applied Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le Jeune, C</au><au>Lonvaud-Funel, A</au><au>Brink, B. ten</au><au>Hofstra, H</au><au>Vossen, J.M.B.M. van der</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test</atitle><jtitle>Journal of Applied Bacteriology</jtitle><addtitle>J Appl Bacteriol</addtitle><date>1995-03</date><risdate>1995</risdate><volume>78</volume><issue>3</issue><spage>316</spage><epage>326</epage><pages>316-326</pages><issn>0021-8847</issn><eissn>2056-5232</eissn><coden>JABAA4</coden><abstract>On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1/JV17HC were also useful for the detection of histamine-forming Leuconostoc oenos strains in PCR. The 150 base pair amplification product of the decarboxylating Leuc. oenos strain generated with primer set CL1/CL2 was sequenced. Alignment studies showed a high degree of relatedness among the hdcA gene products of Gram-positive bacteria. The amplification products of the hdcA genes from Lact. buchneri and Leuc. oenos were used to serve as a DNA probe in hybridization studies. All histidine-decarboxylating lactic acid bacteria gave a hybridization signal with the DNA probes. In hybridization only one false-positive signal with a Lactobacillus lindneri strain was observed, which was anticipated to contain a truncated hdcA gene. In addition to these DNA probe tests, a simple and reliable activity test is presented, which can be used during starter selection to test strains for histidine decarboxylase activity.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7730207</pmid><doi>10.1111/j.1365-2672.1995.tb05032.x</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0021-8847 |
| ispartof | Journal of Applied Bacteriology, 1995-03, Vol.78 (3), p.316-326 |
| issn | 0021-8847 2056-5232 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Amino Acid Sequence amino acid sequences Animals Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences carboxy-lyases Clostridium perfringens - enzymology Clostridium perfringens - genetics Clostridium perfringens - isolation & purification comparisons detection DNA Probes enzyme activity Evaluation Studies as Topic Food industries Food Microbiology Fundamental and applied biological sciences. Psychology genbank/u09485 hdca gene histamine Histamine - metabolism histamine producing lactic acid bacteria histidine Histidine - metabolism histidine decarboxylase Histidine Decarboxylase - genetics Histidine Decarboxylase - metabolism histidine decarboxylation Lactates - metabolism Lactic Acid lactic acid bacteria Lactobacillus - enzymology Lactobacillus - genetics Lactobacillus - isolation & purification Leuconostoc - enzymology Leuconostoc - genetics Microbiology Molecular Sequence Data nucleic acid hybridization nucleotide sequences polymerase chain reaction Polymerase Chain Reaction - methods sequence alignment Sequence Homology, Nucleic Acid structural genes |
| title | Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test |
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